Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA

Detalhes bibliográficos
Autor(a) principal: Lissaldo, Ana Maria
Data de Publicação: 1994
Outros Autores: Hoshino-Shimizu, Sumie, Umezawa, Eufrosina Setsu, Stolf, Anna Maria Simonsen
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29141
Resumo: The alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) was assessed in dot-ELISA for the diagnosis of Chagas' disease. Serum samples (355) from chagasic and non-chagasic patients were studied, and IgG antibodies to ASEA were found in all patients with chronic Chagas' disease. In non-chagasic patients 95.6% were negative, except for those with leishmaniasis (visceral and mucocutaneous), and some patients from control group reacted in low titers. The data indicate that dot-ELISA using ASEA is suitable for seroepidemiologic surveys to be employed in endemic areas for Chagas' disease.
id IMT-1_4d9f44b849bc8304caad8fe73d050f49
oai_identifier_str oai:revistas.usp.br:article/29141
network_acronym_str IMT-1
network_name_str Revista do Instituto de Medicina Tropical de São Paulo
repository_id_str
spelling Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA Antígeno de Epimastigota de Trypanosoma cruzi, solubilizado em meio alcalino (ASEA) aplicado em dot-ELISA Chagas' diseaseEpimastigoteDot-ELISASerodiagnosis The alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) was assessed in dot-ELISA for the diagnosis of Chagas' disease. Serum samples (355) from chagasic and non-chagasic patients were studied, and IgG antibodies to ASEA were found in all patients with chronic Chagas' disease. In non-chagasic patients 95.6% were negative, except for those with leishmaniasis (visceral and mucocutaneous), and some patients from control group reacted in low titers. The data indicate that dot-ELISA using ASEA is suitable for seroepidemiologic surveys to be employed in endemic areas for Chagas' disease. O antígeno de epimastigota do T. cruzi solubilizado em meio alcalino (ASEA) foi avaliado em dot-ELISA para o diagnóstico da doença de Chagas. Amostras de soros (355) de chagásicos e não chagásicos foram estudadas e anticorpos IgG contra ASEA foram encontrados em todos os pacientes com a doença de Chagas crônica. Em pacientes não chagásicos, os resultados foram negativos (95,6%), exceto para aqueles com leishmaniose visceral e mucocutânea, e para alguns do grupo controle que reagiram em títulos baixos. Os dados indicam que o ensaio de dot-ELISA, utilizando o ASEA é apropriado para estudos soroepidemiológicos a serem conduzidos em áreas endêmicas da doença de Chagas, tendo em mente a limitação do teste em áreas onde Doença de Chagas e leishmaniose estão presentes. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1994-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29141Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 No. 2 (1994); 163-166 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 Núm. 2 (1994); 163-166 Revista do Instituto de Medicina Tropical de São Paulo; v. 36 n. 2 (1994); 163-166 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29141/30998Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessLissaldo, Ana MariaHoshino-Shimizu, SumieUmezawa, Eufrosina SetsuStolf, Anna Maria Simonsen2012-07-02T01:37:28Zoai:revistas.usp.br:article/29141Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:53.714224Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
Antígeno de Epimastigota de Trypanosoma cruzi, solubilizado em meio alcalino (ASEA) aplicado em dot-ELISA
title Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
spellingShingle Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
Lissaldo, Ana Maria
Chagas' disease
Epimastigote
Dot-ELISA
Serodiagnosis
title_short Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
title_full Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
title_fullStr Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
title_full_unstemmed Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
title_sort Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
author Lissaldo, Ana Maria
author_facet Lissaldo, Ana Maria
Hoshino-Shimizu, Sumie
Umezawa, Eufrosina Setsu
Stolf, Anna Maria Simonsen
author_role author
author2 Hoshino-Shimizu, Sumie
Umezawa, Eufrosina Setsu
Stolf, Anna Maria Simonsen
author2_role author
author
author
dc.contributor.author.fl_str_mv Lissaldo, Ana Maria
Hoshino-Shimizu, Sumie
Umezawa, Eufrosina Setsu
Stolf, Anna Maria Simonsen
dc.subject.por.fl_str_mv Chagas' disease
Epimastigote
Dot-ELISA
Serodiagnosis
topic Chagas' disease
Epimastigote
Dot-ELISA
Serodiagnosis
description The alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) was assessed in dot-ELISA for the diagnosis of Chagas' disease. Serum samples (355) from chagasic and non-chagasic patients were studied, and IgG antibodies to ASEA were found in all patients with chronic Chagas' disease. In non-chagasic patients 95.6% were negative, except for those with leishmaniasis (visceral and mucocutaneous), and some patients from control group reacted in low titers. The data indicate that dot-ELISA using ASEA is suitable for seroepidemiologic surveys to be employed in endemic areas for Chagas' disease.
publishDate 1994
dc.date.none.fl_str_mv 1994-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29141
url https://www.revistas.usp.br/rimtsp/article/view/29141
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29141/30998
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 No. 2 (1994); 163-166
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 Núm. 2 (1994); 163-166
Revista do Instituto de Medicina Tropical de São Paulo; v. 36 n. 2 (1994); 163-166
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951640620859392

Registros relacionados