COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/105536 |
Resumo: | Infection by Candidaspp. is associated with high mortality rates, especially when treatment is not appropriate and/or not immediate. Therefore, it is necessary to correctly identify the genus and species of Candida. The aim of this study was to compare the identification of 89 samples of Candidaspp. by the manual methods germ tube test, auxanogram and chromogenic medium in relation to the ID 32C automated method. The concordances between the methods in ascending order, measured by the Kappa index were: ID 32C with CHROMagar Candida(κ = 0.38), ID 32C with auxanogram (κ = 0.59) and ID 32C with germ tube (κ = 0.9). One of the species identified in this study was C. tropicalis,which demonstrated a sensitivity of 46.2%, a specificity of 95.2%, PPV of 80%, NPV of 81.1%, and an accuracy of 80.9% in tests performed with CHROMagar Candida;and a sensitivity of 76.9%, a specificity of 96.8%, PPV of 90.9%, NPV of 91%, and an accuracy of 91% in the auxanogram tests. Therefore, it is necessary to know the advantages and limitations of methods to choose the best combination between them for a fast and correct identification of Candidaspecies. |
id |
IMT-1_518ef2a2a7f5370d24be52a36075bcfe |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/105536 |
network_acronym_str |
IMT-1 |
network_name_str |
Revista do Instituto de Medicina Tropical de São Paulo |
repository_id_str |
|
spelling |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES Comparação entre quatro métodos usuais de identificação de espécies de Candida Infection by Candidaspp. is associated with high mortality rates, especially when treatment is not appropriate and/or not immediate. Therefore, it is necessary to correctly identify the genus and species of Candida. The aim of this study was to compare the identification of 89 samples of Candidaspp. by the manual methods germ tube test, auxanogram and chromogenic medium in relation to the ID 32C automated method. The concordances between the methods in ascending order, measured by the Kappa index were: ID 32C with CHROMagar Candida(κ = 0.38), ID 32C with auxanogram (κ = 0.59) and ID 32C with germ tube (κ = 0.9). One of the species identified in this study was C. tropicalis,which demonstrated a sensitivity of 46.2%, a specificity of 95.2%, PPV of 80%, NPV of 81.1%, and an accuracy of 80.9% in tests performed with CHROMagar Candida;and a sensitivity of 76.9%, a specificity of 96.8%, PPV of 90.9%, NPV of 91%, and an accuracy of 91% in the auxanogram tests. Therefore, it is necessary to know the advantages and limitations of methods to choose the best combination between them for a fast and correct identification of Candidaspecies. A infecção por Candidaspp. está associada com alta mortalidade, principalmente quando o tratamento não é adequado, nem imediato. Assim, a correta identificação do gênero e espécie é necessária. O objetivo deste trabalho foi comparar 89 amostras de Candidaspp. pelos métodos manuais prova do tubo germinativo, auxanograma e CHROMagar em relação ao método automatizado ID 32C. As concordâncias entre os métodos em ordem crescente, medidas pelo coeficiente de Kappa, foram: ID 32C com CHROMagar Candida(κ = 0,38), ID 32C com auxanograma (κ = 0,59) e ID 32C com tubo germinativo (κ = 0,9). Uma das espécies identificadas neste trabalho foi a C. tropicalis, que demonstrou uma sensibilidade de 46,2%, especificidade de 95,2%, VPP de 80%, VPN de 81,1% e acurácia de 80,9% nos testes com CHROMagar Candidae uma sensibilidade de 76,9%, especificidade de 96,8%, VPP de 90,9%, VPN de 91% e acurácia de 91% nos testes de auxanograma. Portanto, o conhecimento das vantagens e limitações dos métodos é necessário para a escolha da melhor combinação entre os mesmos visando uma rápida e correta identificação das espécies de Candida. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/105536Revista do Instituto de Medicina Tropical de São Paulo; Vol. 57 No. 4 (2015); 281-287Revista do Instituto de Medicina Tropical de São Paulo; Vol. 57 Núm. 4 (2015); 281-287Revista do Instituto de Medicina Tropical de São Paulo; v. 57 n. 4 (2015); 281-2871678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/105536/104192Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSOUZA, Margarida NevesORTIZ, Stéfanie OtowiczMELLO, Marcelo MartinsOLIVEIRA, Flávio de MattosSEVERO, Luiz CarlosGOEBEL, Cristine Souza2015-10-09T12:54:23Zoai:revistas.usp.br:article/105536Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:33.110835Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES Comparação entre quatro métodos usuais de identificação de espécies de Candida |
title |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES |
spellingShingle |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES SOUZA, Margarida Neves |
title_short |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES |
title_full |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES |
title_fullStr |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES |
title_full_unstemmed |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES |
title_sort |
COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF CandidaSPECIES |
author |
SOUZA, Margarida Neves |
author_facet |
SOUZA, Margarida Neves ORTIZ, Stéfanie Otowicz MELLO, Marcelo Martins OLIVEIRA, Flávio de Mattos SEVERO, Luiz Carlos GOEBEL, Cristine Souza |
author_role |
author |
author2 |
ORTIZ, Stéfanie Otowicz MELLO, Marcelo Martins OLIVEIRA, Flávio de Mattos SEVERO, Luiz Carlos GOEBEL, Cristine Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
SOUZA, Margarida Neves ORTIZ, Stéfanie Otowicz MELLO, Marcelo Martins OLIVEIRA, Flávio de Mattos SEVERO, Luiz Carlos GOEBEL, Cristine Souza |
description |
Infection by Candidaspp. is associated with high mortality rates, especially when treatment is not appropriate and/or not immediate. Therefore, it is necessary to correctly identify the genus and species of Candida. The aim of this study was to compare the identification of 89 samples of Candidaspp. by the manual methods germ tube test, auxanogram and chromogenic medium in relation to the ID 32C automated method. The concordances between the methods in ascending order, measured by the Kappa index were: ID 32C with CHROMagar Candida(κ = 0.38), ID 32C with auxanogram (κ = 0.59) and ID 32C with germ tube (κ = 0.9). One of the species identified in this study was C. tropicalis,which demonstrated a sensitivity of 46.2%, a specificity of 95.2%, PPV of 80%, NPV of 81.1%, and an accuracy of 80.9% in tests performed with CHROMagar Candida;and a sensitivity of 76.9%, a specificity of 96.8%, PPV of 90.9%, NPV of 91%, and an accuracy of 91% in the auxanogram tests. Therefore, it is necessary to know the advantages and limitations of methods to choose the best combination between them for a fast and correct identification of Candidaspecies. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/105536 |
url |
https://www.revistas.usp.br/rimtsp/article/view/105536 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/105536/104192 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 57 No. 4 (2015); 281-287 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 57 Núm. 4 (2015); 281-287 Revista do Instituto de Medicina Tropical de São Paulo; v. 57 n. 4 (2015); 281-287 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
_version_ |
1798951651194699776 |