Detection and characterization of Bacillus cereus isolated from the dialysis fluid

• Autor(a) principal: Sturmer, Fabiana de Cássia Romanha
• Data de Publicação: 2022
• Outros Autores: Moreira, Paulo Ricardo, Cargnelutti, Juliana Felipetto, Lopes, Leonardo Quintana Soares, Lorenzett, Ezequiel, Burgo, Thiago Augusto de Lima, Santos, Roberto Christ Vianna
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
• Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/204563
• topic: Renal dialysis; Centrifuge; Bacillus cereus group; Biofilm

In this study, B. cereus was detected in dialysis fluids within international parameters (ultrapure – maximum limit of 0.1 CFU/mL for heterotrophic bacteria count) by analyzing the pellet obtained through the centrifugation method. We also investigated the ability of the B. cereus isolate to form a biofilm at different temperatures, the production of virulence factors, and the susceptibility to commercial antimicrobial agents. This study demonstrated a high ability of B. cereus to persist in the hemodialysis system, which can be explained by its broad ability to produce a biofilm at 25 °C, its relevant production of virulence factors, such as β-hemolysin, lecithinase and cereulide, and its important resistance pattern to antimicrobial drugs. In conclusion, these new findings expand the understanding that this microorganism should not be neglected and new methods for tracking it should be considered.