Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
Autor(a) principal: | |
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Data de Publicação: | 1995 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29271 |
Resumo: | Eighty purulent cerebrospinal fluid (CSF) samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA) kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE), as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test Detecção de antígenos bacterianos no líquido cefalorraquidiano através do teste de aglutinação de latex Bacterial meningitisLatex agglutinationBacterial antigen detection in CSF Eighty purulent cerebrospinal fluid (CSF) samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA) kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE), as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C. Oitenta amostras purulentas de líquido cefalorraquidiano (LCR) de pacientes com evidência clínica de meningite foram estudadas empregando-se Kit Directigen de aglutinação de latex (AL) para demonstrar antígeno bacteriano no LCR. Os resultados mostraram que o teste de AL apresentou melhor desempenho diagnóstico do que bacterioscopia através da coloração de Gram, cultura e imunoeletroforese cruzada (IEC) em relação à Neisseria meningitidis grupos B e C, e ao Haemophilus influenzae tipo b, e melhor do que coloração de Gram e cultura quando Streptococcus pneumoniae foi avaliado. A comparação dos resultados com os de cultura mostrou o maior nível de sensibilidade considerando-se N. meningitidis grupo C. Quanto à especificidade, os valores foram satisfatórios para todos os microrganismos testados. O grau de concordância K em relação à IEC exibiu melhores índices K de concordância para N. meningitidis grupos B e C. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1995-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29271Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 3 (1995); 257-260 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 3 (1995); 257-260 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 3 (1995); 257-260 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29271/31128Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessLandgraf, Ilka MariaAlkmin, Maria das Graças AdelinoVieira, Maria de Fátima Paiva2012-07-02T01:40:18Zoai:revistas.usp.br:article/29271Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:01.232258Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test Detecção de antígenos bacterianos no líquido cefalorraquidiano através do teste de aglutinação de latex |
title |
Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test |
spellingShingle |
Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test Landgraf, Ilka Maria Bacterial meningitis Latex agglutination Bacterial antigen detection in CSF |
title_short |
Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test |
title_full |
Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test |
title_fullStr |
Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test |
title_full_unstemmed |
Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test |
title_sort |
Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test |
author |
Landgraf, Ilka Maria |
author_facet |
Landgraf, Ilka Maria Alkmin, Maria das Graças Adelino Vieira, Maria de Fátima Paiva |
author_role |
author |
author2 |
Alkmin, Maria das Graças Adelino Vieira, Maria de Fátima Paiva |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Landgraf, Ilka Maria Alkmin, Maria das Graças Adelino Vieira, Maria de Fátima Paiva |
dc.subject.por.fl_str_mv |
Bacterial meningitis Latex agglutination Bacterial antigen detection in CSF |
topic |
Bacterial meningitis Latex agglutination Bacterial antigen detection in CSF |
description |
Eighty purulent cerebrospinal fluid (CSF) samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA) kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE), as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C. |
publishDate |
1995 |
dc.date.none.fl_str_mv |
1995-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29271 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29271 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29271/31128 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 3 (1995); 257-260 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 3 (1995); 257-260 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 3 (1995); 257-260 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951641598132224 |