Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30545 |
Resumo: | Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 ± 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons Piolhos hematófagos podem disseminar infecção pelo Trypanosoma cruzi em babuínos BaboonsPapio hamadriasLicePedicinus obtususTrypanosoma cruzi infection Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 ± 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony. As infecções pelo Trypanosoma cruzi e a doença de Chagas são endêmicas em babuínos (Papio hamadryas) reproduzidos em cativeiro na Southwest Foundation for Biomedical Research, em Santo Antonio, Texas. Nós obtivemos produtos de amplificação por PCR do DNA extraído de piolhos colhidos do cabelo e da pele de babuínos chagásicos, com primers aneladores específicos para DNAs nuclear e de cinetoplasto do protozoário. Esses produtos foram hibridizados com suas respectivas seqüências internas complementares. Seqüências selecionadas foram clonadas e o sequenciamento demonstrou a presença de DNA nuclear de T. cruzi, e de minicírculo de kDNA. A PCR competitiva com primers de kDNA determinou a quantidade de aproximadamente 23.9 ± 18.2 T. cruzi por piolho. Este achado sugere que o piolho pode ser um vetor contribuindo para a disseminação de T. cruzi na colônia de babuínos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2001-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30545Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 271-276 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 271-276 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 271-276 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30545/32429Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessARGAÑARAZ, Enrique R.HUBBARD, Gene B.RAMOS, Larissa A.FORD, Allen L.NITZ, NadjarLELAND, Michelle M.VANDEBERG, John L.TEIXEIRA, Antonio R.L.2012-07-07T13:35:27Zoai:revistas.usp.br:article/30545Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:19.845195Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons Piolhos hematófagos podem disseminar infecção pelo Trypanosoma cruzi em babuínos |
title |
Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons |
spellingShingle |
Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons ARGAÑARAZ, Enrique R. Baboons Papio hamadrias Lice Pedicinus obtusus Trypanosoma cruzi infection |
title_short |
Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons |
title_full |
Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons |
title_fullStr |
Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons |
title_full_unstemmed |
Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons |
title_sort |
Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons |
author |
ARGAÑARAZ, Enrique R. |
author_facet |
ARGAÑARAZ, Enrique R. HUBBARD, Gene B. RAMOS, Larissa A. FORD, Allen L. NITZ, Nadjar LELAND, Michelle M. VANDEBERG, John L. TEIXEIRA, Antonio R.L. |
author_role |
author |
author2 |
HUBBARD, Gene B. RAMOS, Larissa A. FORD, Allen L. NITZ, Nadjar LELAND, Michelle M. VANDEBERG, John L. TEIXEIRA, Antonio R.L. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
ARGAÑARAZ, Enrique R. HUBBARD, Gene B. RAMOS, Larissa A. FORD, Allen L. NITZ, Nadjar LELAND, Michelle M. VANDEBERG, John L. TEIXEIRA, Antonio R.L. |
dc.subject.por.fl_str_mv |
Baboons Papio hamadrias Lice Pedicinus obtusus Trypanosoma cruzi infection |
topic |
Baboons Papio hamadrias Lice Pedicinus obtusus Trypanosoma cruzi infection |
description |
Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 ± 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30545 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30545 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30545/32429 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 271-276 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 271-276 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 271-276 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
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