Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15512 |
Resumo: | Isoprene (C5H8) is a hydrocarbon gas emitted by many tree species and has been shown to protect photosynthesis under abiotic stress. Under optimal conditions for photosynthesis, ~70%-90% of carbon used for isoprene biosynthesis is produced fromrecently assimilated atmospheric CO2. While the contribution of alternative carbon sources that increase with leaf temperature and other stresses have been demonstrated, uncertainties remain regarding the biochemical source(s) of isoprene carbon. In this study, we investigated leaf isoprene emissions (Is) from neotropical species Inga edulis Mart. as a function of light and temperature under ambient (450 μmol m-2 s-1) and CO2-free (0 μmol m-2 s-1) atmosphere. Is under CO2-free atmosphere showed light-dependent emission patterns similar to those observed under ambient CO2, but with lower light saturation point. Leaves treated with the photosynthesis inhibitor DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) failed to produce detectable Is in normal light under a CO2-free atmosphere. While strong temperature-dependent Is were observed under CO2-free atmosphere in the light, dark conditions failed to produce detectable Is even at the highest temperatures studied (40 °C). Treatment of leaves with 13C-labeled sodium bicarbonate under CO2-free atmosphere resulted in Is with over 50% containing at least one 13C atom. Is under CO2-free atmosphere and standard conditions of light and leaf temperature represented 19% ± 7% of emissions under ambient CO2. The results show that the reassimilation of leaf internal CO2 contributes to Is in the neotropical species I. edulis. Through the consumption of excess photosynthetic energy, our results support a role of isoprene biosynthesis, together with photorespiration, as a key tolerance mechanism against high temperature and high light in the tropics. © 2019 by the authors. |
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Garcia, SabrinaJardine, Kolby J.Souza, Vinícius Fernandes deSouza, Rodrigo Augusto Ferreira deDuvoisin Júnior, SérgioGonçalves, José Francisco Carvalho de2020-05-14T16:32:35Z2020-05-14T16:32:35Z2019https://repositorio.inpa.gov.br/handle/1/1551210.3390/f10060472Isoprene (C5H8) is a hydrocarbon gas emitted by many tree species and has been shown to protect photosynthesis under abiotic stress. Under optimal conditions for photosynthesis, ~70%-90% of carbon used for isoprene biosynthesis is produced fromrecently assimilated atmospheric CO2. While the contribution of alternative carbon sources that increase with leaf temperature and other stresses have been demonstrated, uncertainties remain regarding the biochemical source(s) of isoprene carbon. In this study, we investigated leaf isoprene emissions (Is) from neotropical species Inga edulis Mart. as a function of light and temperature under ambient (450 μmol m-2 s-1) and CO2-free (0 μmol m-2 s-1) atmosphere. Is under CO2-free atmosphere showed light-dependent emission patterns similar to those observed under ambient CO2, but with lower light saturation point. Leaves treated with the photosynthesis inhibitor DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) failed to produce detectable Is in normal light under a CO2-free atmosphere. While strong temperature-dependent Is were observed under CO2-free atmosphere in the light, dark conditions failed to produce detectable Is even at the highest temperatures studied (40 °C). Treatment of leaves with 13C-labeled sodium bicarbonate under CO2-free atmosphere resulted in Is with over 50% containing at least one 13C atom. Is under CO2-free atmosphere and standard conditions of light and leaf temperature represented 19% ± 7% of emissions under ambient CO2. The results show that the reassimilation of leaf internal CO2 contributes to Is in the neotropical species I. edulis. Through the consumption of excess photosynthetic energy, our results support a role of isoprene biosynthesis, together with photorespiration, as a key tolerance mechanism against high temperature and high light in the tropics. © 2019 by the authors.Volume 10, Número 6Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBiochemistryBiosynthesisCarbonCarbon DioxideCarboxylationIsoprenePhotosynthesisSodium BicarbonateAbiotic StressCarbon SourceDecarboxylation ProcessHighest TemperaturePhotosynthesis InhibitorStandard ConditionsTemperature DependentTolerance MechanismsAtmospheric TemperatureAbiotic FactorBiogenic EmissionCarbon DioxideDecarboxylationFruitIsopreneLeafPhotosynthesisBiochemistryBiosynthesisCarbon DioxideCarboxylationIsopreneInga EdulisReassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Martinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleForestsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf3812240https://repositorio.inpa.gov.br/bitstream/1/15512/1/artigo-inpa.pdfa3146af2928d3f1f18f44e0ee0328d71MD511/155122020-05-14 15:50:58.931oai:repositorio:1/15512Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-14T19:50:58Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
title |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
spellingShingle |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart Garcia, Sabrina Biochemistry Biosynthesis Carbon Carbon Dioxide Carboxylation Isoprene Photosynthesis Sodium Bicarbonate Abiotic Stress Carbon Source Decarboxylation Process Highest Temperature Photosynthesis Inhibitor Standard Conditions Temperature Dependent Tolerance Mechanisms Atmospheric Temperature Abiotic Factor Biogenic Emission Carbon Dioxide Decarboxylation Fruit Isoprene Leaf Photosynthesis Biochemistry Biosynthesis Carbon Dioxide Carboxylation Isoprene Inga Edulis |
title_short |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
title_full |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
title_fullStr |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
title_full_unstemmed |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
title_sort |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
author |
Garcia, Sabrina |
author_facet |
Garcia, Sabrina Jardine, Kolby J. Souza, Vinícius Fernandes de Souza, Rodrigo Augusto Ferreira de Duvoisin Júnior, Sérgio Gonçalves, José Francisco Carvalho de |
author_role |
author |
author2 |
Jardine, Kolby J. Souza, Vinícius Fernandes de Souza, Rodrigo Augusto Ferreira de Duvoisin Júnior, Sérgio Gonçalves, José Francisco Carvalho de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Garcia, Sabrina Jardine, Kolby J. Souza, Vinícius Fernandes de Souza, Rodrigo Augusto Ferreira de Duvoisin Júnior, Sérgio Gonçalves, José Francisco Carvalho de |
dc.subject.eng.fl_str_mv |
Biochemistry Biosynthesis Carbon Carbon Dioxide Carboxylation Isoprene Photosynthesis Sodium Bicarbonate Abiotic Stress Carbon Source Decarboxylation Process Highest Temperature Photosynthesis Inhibitor Standard Conditions Temperature Dependent Tolerance Mechanisms Atmospheric Temperature Abiotic Factor Biogenic Emission Carbon Dioxide Decarboxylation Fruit Isoprene Leaf Photosynthesis Biochemistry Biosynthesis Carbon Dioxide Carboxylation Isoprene Inga Edulis |
topic |
Biochemistry Biosynthesis Carbon Carbon Dioxide Carboxylation Isoprene Photosynthesis Sodium Bicarbonate Abiotic Stress Carbon Source Decarboxylation Process Highest Temperature Photosynthesis Inhibitor Standard Conditions Temperature Dependent Tolerance Mechanisms Atmospheric Temperature Abiotic Factor Biogenic Emission Carbon Dioxide Decarboxylation Fruit Isoprene Leaf Photosynthesis Biochemistry Biosynthesis Carbon Dioxide Carboxylation Isoprene Inga Edulis |
description |
Isoprene (C5H8) is a hydrocarbon gas emitted by many tree species and has been shown to protect photosynthesis under abiotic stress. Under optimal conditions for photosynthesis, ~70%-90% of carbon used for isoprene biosynthesis is produced fromrecently assimilated atmospheric CO2. While the contribution of alternative carbon sources that increase with leaf temperature and other stresses have been demonstrated, uncertainties remain regarding the biochemical source(s) of isoprene carbon. In this study, we investigated leaf isoprene emissions (Is) from neotropical species Inga edulis Mart. as a function of light and temperature under ambient (450 μmol m-2 s-1) and CO2-free (0 μmol m-2 s-1) atmosphere. Is under CO2-free atmosphere showed light-dependent emission patterns similar to those observed under ambient CO2, but with lower light saturation point. Leaves treated with the photosynthesis inhibitor DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) failed to produce detectable Is in normal light under a CO2-free atmosphere. While strong temperature-dependent Is were observed under CO2-free atmosphere in the light, dark conditions failed to produce detectable Is even at the highest temperatures studied (40 °C). Treatment of leaves with 13C-labeled sodium bicarbonate under CO2-free atmosphere resulted in Is with over 50% containing at least one 13C atom. Is under CO2-free atmosphere and standard conditions of light and leaf temperature represented 19% ± 7% of emissions under ambient CO2. The results show that the reassimilation of leaf internal CO2 contributes to Is in the neotropical species I. edulis. Through the consumption of excess photosynthetic energy, our results support a role of isoprene biosynthesis, together with photorespiration, as a key tolerance mechanism against high temperature and high light in the tropics. © 2019 by the authors. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-05-14T16:32:35Z |
dc.date.available.fl_str_mv |
2020-05-14T16:32:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15512 |
dc.identifier.doi.none.fl_str_mv |
10.3390/f10060472 |
url |
https://repositorio.inpa.gov.br/handle/1/15512 |
identifier_str_mv |
10.3390/f10060472 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 10, Número 6 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Forests |
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Forests |
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Repositório Institucional do INPA |
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