Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/14652 |
Resumo: | Lebiasinidae is a small fish family composed by miniature to small-sized fishes with few cytogenetic data (most of them limited to descriptions of diploid chromosome numbers), thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing, the first cytogenetic data for the red spotted tetra, Copeina guttata, including the standard karyotype, C-banding, repetitive DNA mapping by fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH), providing chromosomal patterns and novel insights into the karyotype differentiation of the family. Males and females share diploid chromosome number 2n = 42 and karyotype composed of 2 metacentric (m), 4 submetacentric (sm) and 36 subtelocentric to acrocentric (st-a) chromosomes. Blocks of constitutive heterochromatin were observed in the centromeric and interstitial regions of several chromosomes, in addition to a remarkably large distal block, heteromorphic in size, which fully corresponded with the 18S rDNA sites in the fourth chromosomal pair. This overlap was confirmed by 5S/18S rDNA dual-color FISH. On the other hand, 5S rDNA clusters were situated in the long and short arms of the 2nd and 15th pairs, respectively. No sex-linked karyotype differences were revealed by male/female CGH experiments. The genomic probes from other two lebiasinid species, Lebiasina melanoguttata and Pyrrhulina brevis, showed positive hybridization signals only in the NOR region in the genome of C. guttata. We demonstrated that karyotype diversification in lebiasinids was accompanied by a series of structural and numeric chromosome rearrangements of different types, including particularly fusions and fissions. © 2019 Toma et al. |
id |
INPA-2_ca071c6077bf98ecc60d5d4fc2cac081 |
---|---|
oai_identifier_str |
oai:repositorio:1/14652 |
network_acronym_str |
INPA-2 |
network_name_str |
Repositório Institucional do INPA |
repository_id_str |
|
spelling |
Toma, Gustavo AkiraMoraes, Renata Luiza Rosa deSassi, Francisco de Menezes CavalcanteBertollo, Luiz Antônio CarlosOliveira, Ezequiel Aguiar deRáb, PetrSember, AlexandrLiehr, ThomasHatanaka, TerumiViana, Patrik FerreiraMarinho, Manoela Maria FerreiraFeldberg, ElianaCioffi, Marcelo de Bello2020-04-24T16:59:56Z2020-04-24T16:59:56Z2019https://repositorio.inpa.gov.br/handle/1/1465210.1371/journal.pone.0226746Lebiasinidae is a small fish family composed by miniature to small-sized fishes with few cytogenetic data (most of them limited to descriptions of diploid chromosome numbers), thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing, the first cytogenetic data for the red spotted tetra, Copeina guttata, including the standard karyotype, C-banding, repetitive DNA mapping by fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH), providing chromosomal patterns and novel insights into the karyotype differentiation of the family. Males and females share diploid chromosome number 2n = 42 and karyotype composed of 2 metacentric (m), 4 submetacentric (sm) and 36 subtelocentric to acrocentric (st-a) chromosomes. Blocks of constitutive heterochromatin were observed in the centromeric and interstitial regions of several chromosomes, in addition to a remarkably large distal block, heteromorphic in size, which fully corresponded with the 18S rDNA sites in the fourth chromosomal pair. This overlap was confirmed by 5S/18S rDNA dual-color FISH. On the other hand, 5S rDNA clusters were situated in the long and short arms of the 2nd and 15th pairs, respectively. No sex-linked karyotype differences were revealed by male/female CGH experiments. The genomic probes from other two lebiasinid species, Lebiasina melanoguttata and Pyrrhulina brevis, showed positive hybridization signals only in the NOR region in the genome of C. guttata. We demonstrated that karyotype diversification in lebiasinids was accompanied by a series of structural and numeric chromosome rearrangements of different types, including particularly fusions and fissions. © 2019 Toma et al.Volume 14, Número 12Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessDna 18sDna 5sRepetitive DnaRibosome RnaAnimals ExperimentC BandingCentromereCharaciformesChromosome ArmChromosome Banding PatternChromosome NorChromosome RearrangementComparative Genomic HybridizationControlled StudyCopeina GuttataCytogeneticsDiploidyDna DeterminationFemaleIn Situ Hybridization, FluorescenceGiemsa StainHeterochromatinKaryotypeLebiasina MelanoguttataLebiasinidaeMaleNonhumanPyrrhulina BrevisRed Spotted TetraAnimalsCharaciformesChromosomeClassificationGeneticsEvolution, MolecularPhylogenyAnimalssCharaciformesChromosomesEvolution, MolecularFemaleKaryotypeMalePhylogenyRna, RibosomalCytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the familyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLoS ONEengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf1168235https://repositorio.inpa.gov.br/bitstream/1/14652/1/artigo-inpa.pdf50e19ef3c1071dba070f54398772d3e9MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14652/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/146522020-07-14 09:18:54.762oai:repositorio:1/14652Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T13:18:54Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
title |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
spellingShingle |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family Toma, Gustavo Akira Dna 18s Dna 5s Repetitive Dna Ribosome Rna Animals Experiment C Banding Centromere Characiformes Chromosome Arm Chromosome Banding Pattern Chromosome Nor Chromosome Rearrangement Comparative Genomic Hybridization Controlled Study Copeina Guttata Cytogenetics Diploidy Dna Determination Female In Situ Hybridization, Fluorescence Giemsa Stain Heterochromatin Karyotype Lebiasina Melanoguttata Lebiasinidae Male Nonhuman Pyrrhulina Brevis Red Spotted Tetra Animals Characiformes Chromosome Classification Genetics Evolution, Molecular Phylogeny Animalss Characiformes Chromosomes Evolution, Molecular Female Karyotype Male Phylogeny Rna, Ribosomal |
title_short |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
title_full |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
title_fullStr |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
title_full_unstemmed |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
title_sort |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
author |
Toma, Gustavo Akira |
author_facet |
Toma, Gustavo Akira Moraes, Renata Luiza Rosa de Sassi, Francisco de Menezes Cavalcante Bertollo, Luiz Antônio Carlos Oliveira, Ezequiel Aguiar de Ráb, Petr Sember, Alexandr Liehr, Thomas Hatanaka, Terumi Viana, Patrik Ferreira Marinho, Manoela Maria Ferreira Feldberg, Eliana Cioffi, Marcelo de Bello |
author_role |
author |
author2 |
Moraes, Renata Luiza Rosa de Sassi, Francisco de Menezes Cavalcante Bertollo, Luiz Antônio Carlos Oliveira, Ezequiel Aguiar de Ráb, Petr Sember, Alexandr Liehr, Thomas Hatanaka, Terumi Viana, Patrik Ferreira Marinho, Manoela Maria Ferreira Feldberg, Eliana Cioffi, Marcelo de Bello |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Toma, Gustavo Akira Moraes, Renata Luiza Rosa de Sassi, Francisco de Menezes Cavalcante Bertollo, Luiz Antônio Carlos Oliveira, Ezequiel Aguiar de Ráb, Petr Sember, Alexandr Liehr, Thomas Hatanaka, Terumi Viana, Patrik Ferreira Marinho, Manoela Maria Ferreira Feldberg, Eliana Cioffi, Marcelo de Bello |
dc.subject.eng.fl_str_mv |
Dna 18s Dna 5s Repetitive Dna Ribosome Rna Animals Experiment C Banding Centromere Characiformes Chromosome Arm Chromosome Banding Pattern Chromosome Nor Chromosome Rearrangement Comparative Genomic Hybridization Controlled Study Copeina Guttata Cytogenetics Diploidy Dna Determination Female In Situ Hybridization, Fluorescence Giemsa Stain Heterochromatin Karyotype Lebiasina Melanoguttata Lebiasinidae Male Nonhuman Pyrrhulina Brevis Red Spotted Tetra Animals Characiformes Chromosome Classification Genetics Evolution, Molecular Phylogeny Animalss Characiformes Chromosomes Evolution, Molecular Female Karyotype Male Phylogeny Rna, Ribosomal |
topic |
Dna 18s Dna 5s Repetitive Dna Ribosome Rna Animals Experiment C Banding Centromere Characiformes Chromosome Arm Chromosome Banding Pattern Chromosome Nor Chromosome Rearrangement Comparative Genomic Hybridization Controlled Study Copeina Guttata Cytogenetics Diploidy Dna Determination Female In Situ Hybridization, Fluorescence Giemsa Stain Heterochromatin Karyotype Lebiasina Melanoguttata Lebiasinidae Male Nonhuman Pyrrhulina Brevis Red Spotted Tetra Animals Characiformes Chromosome Classification Genetics Evolution, Molecular Phylogeny Animalss Characiformes Chromosomes Evolution, Molecular Female Karyotype Male Phylogeny Rna, Ribosomal |
description |
Lebiasinidae is a small fish family composed by miniature to small-sized fishes with few cytogenetic data (most of them limited to descriptions of diploid chromosome numbers), thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing, the first cytogenetic data for the red spotted tetra, Copeina guttata, including the standard karyotype, C-banding, repetitive DNA mapping by fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH), providing chromosomal patterns and novel insights into the karyotype differentiation of the family. Males and females share diploid chromosome number 2n = 42 and karyotype composed of 2 metacentric (m), 4 submetacentric (sm) and 36 subtelocentric to acrocentric (st-a) chromosomes. Blocks of constitutive heterochromatin were observed in the centromeric and interstitial regions of several chromosomes, in addition to a remarkably large distal block, heteromorphic in size, which fully corresponded with the 18S rDNA sites in the fourth chromosomal pair. This overlap was confirmed by 5S/18S rDNA dual-color FISH. On the other hand, 5S rDNA clusters were situated in the long and short arms of the 2nd and 15th pairs, respectively. No sex-linked karyotype differences were revealed by male/female CGH experiments. The genomic probes from other two lebiasinid species, Lebiasina melanoguttata and Pyrrhulina brevis, showed positive hybridization signals only in the NOR region in the genome of C. guttata. We demonstrated that karyotype diversification in lebiasinids was accompanied by a series of structural and numeric chromosome rearrangements of different types, including particularly fusions and fissions. © 2019 Toma et al. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-04-24T16:59:56Z |
dc.date.available.fl_str_mv |
2020-04-24T16:59:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/14652 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0226746 |
url |
https://repositorio.inpa.gov.br/handle/1/14652 |
identifier_str_mv |
10.1371/journal.pone.0226746 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 14, Número 12 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
PLoS ONE |
publisher.none.fl_str_mv |
PLoS ONE |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/14652/1/artigo-inpa.pdf https://repositorio.inpa.gov.br/bitstream/1/14652/2/license_rdf |
bitstream.checksum.fl_str_mv |
50e19ef3c1071dba070f54398772d3e9 4d2950bda3d176f570a9f8b328dfbbef |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
|
_version_ |
1809928883082887168 |