Distribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil

• Autor(a) principal: Queiroz, Casley B.
• Data de Publicação: 2013
• Outros Autores: Miranda, E. C., Hanada, Rogério Eiji, Sousa, Nelcimar Reis, Gasparotto, Luadir, Soares, Marcos A., Silva, G. F.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/14995
• topic: Bacteriophage Dna; Fungal Protein; Microsatellite Dna; Allele; Banana; Brasil; Controlled Study; Dna Fingerprinting; Fungal Gene; Fungal Plant Disease; Fungus Isolation; Gene Sequence; Genetic Distance; Genetic Marker; Polymorphism, Genetic; Genetic Similarity; Genetic Variability; Geographic Origin; Inter Simple Sequence Repeat; Mat1 1 Gene; Mat1 2 Gene; Mating Type; Mycosphaerella; Mycosphaerella Fijiensis; Nonhuman; Plant Leaf; Polymerase Chain Reaction; Retroposon; Species Distribution; Allele; Ascomycetes; Bacteriophage M13; Fungal Gene; Genetics; Methodology; Microbiology; Musa; Plant Disease; Variable Number Of Tandem Repeat; Alleles; Ascomycota; Bacteriophage M13; Brasil; Fungal Proteins; Genes, Mating Type, Fungal; Genetic Markers; Microsatellite Repeats; Minisatellite Repeats; Musa; Plant Diseases; Plant Leaves; Polymerase Chain Reaction; Polymorphism, Genetic

The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating-type alleles, indicating MAT1-1 and MAT1-2 idiomorphs. A polymorphism analysis using three different primers for M13 markers showed that only the M13 minisatellite primers generated polymorphic products. We then utilized this polymorphism to characterize 40 isolates from various Brazilian states. The largest genetic distances were found between isolates from the same location and between isolates from different parts of the country. Therefore, there was no correlation between the genetic similarity and the geographic origin of the isolates. The M13 marker was used to generate genetic fingerprints for five isolates; these fingerprints were compared with the band profiles obtained from inter-simple sequence repeat (UBC861) and inter-retrotransposon amplified polymorphism analyses. We found that the M13 marker was more effective than the other two markers for differentiating these isolates. © FUNPEC-RP.