Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/14717 |
Resumo: | The critically endangered Central American River Turtle (Dermatemys mawii) is the only remaining member of the Dermatemydidae family, yet little is known about its population structuring. In a previous study of mitochondrial (mt) DNA in the species, three main lineages were described. One lineage (Central) was dominant across most of the range, while two other lineages were restricted to Papaloapan (PAP; isolated by the Isthmus of Tehuantepec and the Sierra de Santa Marta) or the south-eastern part of the range (1D). Here we provide data from seven polymorphic microsatellite loci and the R35 intron to re-evaluate these findings using DNA from the nuclear genome. Based on a slightly expanded data set of a total of 253 samples from the same localities, we find that mtDNA and nuclear DNA markers yield a highly congruent picture of the evolutionary history and population structuring of D. mawii. While resolution provided by the R35 intron (sequenced for a subset of the samples) was very limited, the microsatellite data revealed pronounced population structuring. Within the Grijalva-Usumacinta drainage basin, however, many populations separated by more than 300 kilometers showed signals of high gene flow. Across the entire range, neither mitochondrial nor nuclear DNA show a significant isolation-by-distance pattern, but both genomes highlight that the D. mawii population in the Papaloapan basin is genetically distinctive. Further, both marker systems detect unique genomic signals in four individuals with mtDNA clade 1D sampled on the southeast edge of the Grijalva-Usumacinta basin. These individuals may represent a separate cryptic taxon that is likely impacted by recent admixture. © 2013. |
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González-Porter, Gracia P.Maldonado, Jesus E.Flores-Villela, Oscar A.Vogt, Richard CarlJanke, AxelFleischer, Robert C.Hailer, Frank2020-04-24T17:00:48Z2020-04-24T17:00:48Z2013https://repositorio.inpa.gov.br/handle/1/1471710.1371/journal.pone.0071668The critically endangered Central American River Turtle (Dermatemys mawii) is the only remaining member of the Dermatemydidae family, yet little is known about its population structuring. In a previous study of mitochondrial (mt) DNA in the species, three main lineages were described. One lineage (Central) was dominant across most of the range, while two other lineages were restricted to Papaloapan (PAP; isolated by the Isthmus of Tehuantepec and the Sierra de Santa Marta) or the south-eastern part of the range (1D). Here we provide data from seven polymorphic microsatellite loci and the R35 intron to re-evaluate these findings using DNA from the nuclear genome. Based on a slightly expanded data set of a total of 253 samples from the same localities, we find that mtDNA and nuclear DNA markers yield a highly congruent picture of the evolutionary history and population structuring of D. mawii. While resolution provided by the R35 intron (sequenced for a subset of the samples) was very limited, the microsatellite data revealed pronounced population structuring. Within the Grijalva-Usumacinta drainage basin, however, many populations separated by more than 300 kilometers showed signals of high gene flow. Across the entire range, neither mitochondrial nor nuclear DNA show a significant isolation-by-distance pattern, but both genomes highlight that the D. mawii population in the Papaloapan basin is genetically distinctive. Further, both marker systems detect unique genomic signals in four individuals with mtDNA clade 1D sampled on the southeast edge of the Grijalva-Usumacinta basin. These individuals may represent a separate cryptic taxon that is likely impacted by recent admixture. © 2013.Volume 8, Número 9Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCell Nucleus DnaDna, MitochondrialCentral AmericaCladisticsControlled StudyDermatemys MawiiDna DeterminationEndangered SpeciesEvolutionary Developmental BiologyGene FlowGene FrequencyGene LocusGene SequenceGenetic ConservationGenetic CorrelationGenetic VariabilityGenome AnalysisGeographic DistributionGeographic OriginIntronMicrosatellite MarkerNonhumanNucleotide SequencePhylogenyPopulation StructureRiver EcosystemTurtleAnimalssCentral AmericaDna, MitochondrialEndangered SpeciesGene FlowGenetic VariationPopulation DynamicsTurtlesCryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLoS ONEengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf1276948https://repositorio.inpa.gov.br/bitstream/1/14717/1/artigo-inpa.pdf7a6a075fa518aa88581a37ee8c2607aeMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14717/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/147172020-07-14 10:16:40.999oai:repositorio:1/14717Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:16:40Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle |
title |
Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle |
spellingShingle |
Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle González-Porter, Gracia P. Cell Nucleus Dna Dna, Mitochondrial Central America Cladistics Controlled Study Dermatemys Mawii Dna Determination Endangered Species Evolutionary Developmental Biology Gene Flow Gene Frequency Gene Locus Gene Sequence Genetic Conservation Genetic Correlation Genetic Variability Genome Analysis Geographic Distribution Geographic Origin Intron Microsatellite Marker Nonhuman Nucleotide Sequence Phylogeny Population Structure River Ecosystem Turtle Animalss Central America Dna, Mitochondrial Endangered Species Gene Flow Genetic Variation Population Dynamics Turtles |
title_short |
Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle |
title_full |
Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle |
title_fullStr |
Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle |
title_full_unstemmed |
Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle |
title_sort |
Cryptic Population Structuring and the Role of the Isthmus of Tehuantepec as a Gene Flow Barrier in the Critically Endangered Central American River Turtle |
author |
González-Porter, Gracia P. |
author_facet |
González-Porter, Gracia P. Maldonado, Jesus E. Flores-Villela, Oscar A. Vogt, Richard Carl Janke, Axel Fleischer, Robert C. Hailer, Frank |
author_role |
author |
author2 |
Maldonado, Jesus E. Flores-Villela, Oscar A. Vogt, Richard Carl Janke, Axel Fleischer, Robert C. Hailer, Frank |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
González-Porter, Gracia P. Maldonado, Jesus E. Flores-Villela, Oscar A. Vogt, Richard Carl Janke, Axel Fleischer, Robert C. Hailer, Frank |
dc.subject.eng.fl_str_mv |
Cell Nucleus Dna Dna, Mitochondrial Central America Cladistics Controlled Study Dermatemys Mawii Dna Determination Endangered Species Evolutionary Developmental Biology Gene Flow Gene Frequency Gene Locus Gene Sequence Genetic Conservation Genetic Correlation Genetic Variability Genome Analysis Geographic Distribution Geographic Origin Intron Microsatellite Marker Nonhuman Nucleotide Sequence Phylogeny Population Structure River Ecosystem Turtle Animalss Central America Dna, Mitochondrial Endangered Species Gene Flow Genetic Variation Population Dynamics Turtles |
topic |
Cell Nucleus Dna Dna, Mitochondrial Central America Cladistics Controlled Study Dermatemys Mawii Dna Determination Endangered Species Evolutionary Developmental Biology Gene Flow Gene Frequency Gene Locus Gene Sequence Genetic Conservation Genetic Correlation Genetic Variability Genome Analysis Geographic Distribution Geographic Origin Intron Microsatellite Marker Nonhuman Nucleotide Sequence Phylogeny Population Structure River Ecosystem Turtle Animalss Central America Dna, Mitochondrial Endangered Species Gene Flow Genetic Variation Population Dynamics Turtles |
description |
The critically endangered Central American River Turtle (Dermatemys mawii) is the only remaining member of the Dermatemydidae family, yet little is known about its population structuring. In a previous study of mitochondrial (mt) DNA in the species, three main lineages were described. One lineage (Central) was dominant across most of the range, while two other lineages were restricted to Papaloapan (PAP; isolated by the Isthmus of Tehuantepec and the Sierra de Santa Marta) or the south-eastern part of the range (1D). Here we provide data from seven polymorphic microsatellite loci and the R35 intron to re-evaluate these findings using DNA from the nuclear genome. Based on a slightly expanded data set of a total of 253 samples from the same localities, we find that mtDNA and nuclear DNA markers yield a highly congruent picture of the evolutionary history and population structuring of D. mawii. While resolution provided by the R35 intron (sequenced for a subset of the samples) was very limited, the microsatellite data revealed pronounced population structuring. Within the Grijalva-Usumacinta drainage basin, however, many populations separated by more than 300 kilometers showed signals of high gene flow. Across the entire range, neither mitochondrial nor nuclear DNA show a significant isolation-by-distance pattern, but both genomes highlight that the D. mawii population in the Papaloapan basin is genetically distinctive. Further, both marker systems detect unique genomic signals in four individuals with mtDNA clade 1D sampled on the southeast edge of the Grijalva-Usumacinta basin. These individuals may represent a separate cryptic taxon that is likely impacted by recent admixture. © 2013. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2020-04-24T17:00:48Z |
dc.date.available.fl_str_mv |
2020-04-24T17:00:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/14717 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0071668 |
url |
https://repositorio.inpa.gov.br/handle/1/14717 |
identifier_str_mv |
10.1371/journal.pone.0071668 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Volume 8, Número 9 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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PLoS ONE |
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PLoS ONE |
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