Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1836 |
Resumo: | The aim of the present study was to evaluate the impact of combined exercise training on the development of cardiovascular and neuroimmune dysfunctions induced by fructose overload in sedentary hypertensive (SH), SHR + fructose (HF) and SHR + fructose + training (Treadmill + ladder, 60 days, 40-60% of maximal capacity) (HFTC). The groups were divided into subgroups evaluated at 7, 15, 30 and 60 days (n=6/group/time). Fructose was offered in drinking water (10%). Metabolic, hemodynamic, autonomic, inflammatory, and oxidative stress parameters were evaluated. Regarding the metabolic profile, the HF group showed increase in white adipose tissue in relation to the H group and the HFTC group diminished these values values at 60 days (HF: 1.91?? 0.10 vs. H: 1.61??0.11 and HFTC: 1.42??0.13 g). The HF group presented reduction in insulin sensitivity (HF: 3.15??0.2 vs. H: 3.96??0,1 and HFTC: 4.32??0.2 %/min) at 60 days in relation to H and HFTC groups. The HF group increased triglycerides (TG) when compared to H group at 60 days of protocol (HF:139??7 vs. H: 106??5 and HFTC:107??8 mg/dL). TG were lower in the HFTC group than in the HF group at 60 days. Fructose consumption (HF) induced a further increase in mean arterial pressure (MAP) at 30 and 60 days in SHR (HF-30 days: 153???4 e HF-60 days: 184??4 vs. H-30 days: 141???3 e H-60 days: 165???3 mmHg). On the other hand, combined exercise training reduced MAP in 30 and 60 days of fructose overload in relation to the HF group (HFTC: 142??8 and 167??6 mmHg). There was a decrease on heart rate variability (VAR-PI) in 7 days and 60 days in the HF group (10.5??1.3 and 40.81?? 6.12 ms2) in relation to the H group (23??1.5 and 59.7 ??3.4 ms2); the HFTC group did not presented this impairment (26.8??2.1 and 70.4??5.9 ms2). In addition, the HF group had a lower alpha index (spontaneous baroreflex) at 7 days compared to the H group (0.23??0.03 vs. 0.35??0.01 ms/mmHg), which was not observed in the HFTC group. There was an increase in IL-6 and TNF?? in cardiac tissue at 15, 30 and 60 days in the HF and HFTC groups compared to the H groups. The HFTC group presented higher IL-10 values in the heart at 7 days compared to the HF group (28??1 vs. 16??1 pg/mg of protein). The HF group presented increase in cardiac lipoperoxidation at 30 and 60 days in relation to the H group. The HFTC group decreased lipoperoxidation compared to HF group at 60 days (1302??58 vs. 1956??215 cps/mg protein) and there was also reduction in protein oxidation in this time. In addition, there was an increase in NADPH oxidase in the HF group at 60 days when compared to the H group and reduction in this parameter in the HFTC group in relation to the HF group at the same time (H: 0.18??0.02; HF: 0.46 ??0.04; HFTC 0.35 ??0.03 ??m/mg protein). Considering the redox balance, the HFTC group presented increase in this evaluation in 7, 15, 30 and 60 days in relation to H and HF groups. Our results show that only 7 days of fructose consumption impaired the autonomic control of the circulation, followed by reduction in plasma nitrites and increase in cardiac IL-6 and TNF-??? in 15 days, culminating in increased cardiac protein and lipids damage, which are probably associated with the appearance of cardiometabolic dysfunctions after 30 days of protocol in SHR. In addition, combined exercise training prevented the development of autonomic dysfunction in this model, which probably promoted favorable neuroimmune changes and oxidative stress profile, culminating in a marked attenuation of cardiometabolic dysfunctions in SHR submitted to fructose consumption. Together our findings reinforce the role of the autonomic nervous system in the genesis of cardiometabolic dysfunctions and evidence an important role of combined exercise training in the prevention of these alterations. |
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Angelis, K??tia deAngelis, K??tia deTrombetta, Ivani CredidioCorso, Simone DalIrigoyen, Maria ClaudiaRodrigues, Brunohttp://lattes.cnpq.br/6644420445148028Dias, Danielle da Silva2018-07-16T20:49:37Z2017-10-19Dias, Danielle da Silva. Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos. 2017. 171 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1836The aim of the present study was to evaluate the impact of combined exercise training on the development of cardiovascular and neuroimmune dysfunctions induced by fructose overload in sedentary hypertensive (SH), SHR + fructose (HF) and SHR + fructose + training (Treadmill + ladder, 60 days, 40-60% of maximal capacity) (HFTC). The groups were divided into subgroups evaluated at 7, 15, 30 and 60 days (n=6/group/time). Fructose was offered in drinking water (10%). Metabolic, hemodynamic, autonomic, inflammatory, and oxidative stress parameters were evaluated. Regarding the metabolic profile, the HF group showed increase in white adipose tissue in relation to the H group and the HFTC group diminished these values values at 60 days (HF: 1.91?? 0.10 vs. H: 1.61??0.11 and HFTC: 1.42??0.13 g). The HF group presented reduction in insulin sensitivity (HF: 3.15??0.2 vs. H: 3.96??0,1 and HFTC: 4.32??0.2 %/min) at 60 days in relation to H and HFTC groups. The HF group increased triglycerides (TG) when compared to H group at 60 days of protocol (HF:139??7 vs. H: 106??5 and HFTC:107??8 mg/dL). TG were lower in the HFTC group than in the HF group at 60 days. Fructose consumption (HF) induced a further increase in mean arterial pressure (MAP) at 30 and 60 days in SHR (HF-30 days: 153???4 e HF-60 days: 184??4 vs. H-30 days: 141???3 e H-60 days: 165???3 mmHg). On the other hand, combined exercise training reduced MAP in 30 and 60 days of fructose overload in relation to the HF group (HFTC: 142??8 and 167??6 mmHg). There was a decrease on heart rate variability (VAR-PI) in 7 days and 60 days in the HF group (10.5??1.3 and 40.81?? 6.12 ms2) in relation to the H group (23??1.5 and 59.7 ??3.4 ms2); the HFTC group did not presented this impairment (26.8??2.1 and 70.4??5.9 ms2). In addition, the HF group had a lower alpha index (spontaneous baroreflex) at 7 days compared to the H group (0.23??0.03 vs. 0.35??0.01 ms/mmHg), which was not observed in the HFTC group. There was an increase in IL-6 and TNF?? in cardiac tissue at 15, 30 and 60 days in the HF and HFTC groups compared to the H groups. The HFTC group presented higher IL-10 values in the heart at 7 days compared to the HF group (28??1 vs. 16??1 pg/mg of protein). The HF group presented increase in cardiac lipoperoxidation at 30 and 60 days in relation to the H group. The HFTC group decreased lipoperoxidation compared to HF group at 60 days (1302??58 vs. 1956??215 cps/mg protein) and there was also reduction in protein oxidation in this time. In addition, there was an increase in NADPH oxidase in the HF group at 60 days when compared to the H group and reduction in this parameter in the HFTC group in relation to the HF group at the same time (H: 0.18??0.02; HF: 0.46 ??0.04; HFTC 0.35 ??0.03 ??m/mg protein). Considering the redox balance, the HFTC group presented increase in this evaluation in 7, 15, 30 and 60 days in relation to H and HF groups. Our results show that only 7 days of fructose consumption impaired the autonomic control of the circulation, followed by reduction in plasma nitrites and increase in cardiac IL-6 and TNF-??? in 15 days, culminating in increased cardiac protein and lipids damage, which are probably associated with the appearance of cardiometabolic dysfunctions after 30 days of protocol in SHR. In addition, combined exercise training prevented the development of autonomic dysfunction in this model, which probably promoted favorable neuroimmune changes and oxidative stress profile, culminating in a marked attenuation of cardiometabolic dysfunctions in SHR submitted to fructose consumption. Together our findings reinforce the role of the autonomic nervous system in the genesis of cardiometabolic dysfunctions and evidence an important role of combined exercise training in the prevention of these alterations.O objetivo do presente estudo foi avaliar o impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos (SHR). Para isto SHR foram divididos em grupos: SHR (H), SHR+frutose (HF) e SHR+frutose+treinamento f??sico combinado (esteira+escada, 60 dias, 40-60% da capacidade m??xima) (HFTC). A frutose foi oferecida na ??gua de beber (10%). Par??metros metab??licos, hemodin??micos, auton??micos, inflama????o e estresse oxidativo foram avaliados em subgrupos (n=6 grupo/tempo) em 7, 15, 30 e 60 dias. Com rela????o ao perfil metab??lico o grupo HF aumentou o peso do tecido adiposo branco em rela????o ao grupo H e o grupo HFTC reduziu esses valores em 60 dias (HF: 1,91?? 0,10 vs. H: 1,61??0,11 e HFTC: 1,42??0,13 g). Houve redu????o de sensibilidade ?? insulina no grupo HF em 60 dias (HF: 3,15??0,2 vs. H:3,96??0,1 e HFTC: 4,32??0,2 %/min) em rela????o aos grupos H e HFTC. O grupo HF apresentou aumento de triglic??rides (TG) quando comparado ao grupo H em 60 dias protocolo (HF:139??7 vs. H: 106??5 e HFTC:107??8 mg/dl), o que n??o foi observado no grupo HFTC. O consumo de frutose (grupos HF) induziu um aumento adicional na press??o arterial m??dia (PAM) em 30 e 60 dias de protocolo nos SHR (HF-30 dias: 153???4 e HF-60 dias: 184??4 vs. H-30 dias: 141???3 e H-60 dias: 165???3 mmHg). Por outro lado, o treinamento f??sico combinado (HFTC: 142??8 e 167??6 mmHg) reduziu a PAM em 30 e 60 dias de sobrecarga de frutose em rela????o aos grupos HF. N??o foram observadas diferen??as na frequ??ncia card??aca basal entre os grupos. Houve diminui????o da variabilidade da frequ??ncia card??aca (VAR-IP) em 7 dias e em 60 dias nos grupos HF (10,5???1,3 e 40,81???6,12 ms2) em rela????o aos grupos H (23???1,5 e 59,7???3,4 ms2); o grupo HFTC n??o apresentou esse preju??zo (26,8???2,1 e 70,4???5,9 ms2). Al??m disso, o grupo HF apresentou menor ??ndice alfa (barorreflexo espont??neo) em 7 dias em rela????o ao grupo H (0,23???0,03vs. 0,35???0,01 ms/mmHg), o que n??o foi observado no grupo HFTC. Houve aumento de IL-6 e TNF?? no cora????o em 15, 30 e 60 dias nos grupos HF e HFTC em rela????o aos respectivos grupos H. O grupo HFTC apresentou maiores valores de IL-10 card??aco em 7 dias em rela????o ao grupo HF (28???1 vs. 16???1 pg/mg de prote??na). Os grupos HF apresentaram aumento de lipoperoxida????o card??aca em 30 e 60 dias em rela????o aos grupos H; o grupo HFTC diminuiu a lipoperoxida????o em rela????o ao grupo HF em 60 dias (1302???58 vs.1956???215 cps/mg prote??na), al??m de reduzir a oxida????o de prote??nas em tecido card??aco nesse mesmo tempo. Adicionalmente, houve um aumento na NADPH oxidase no grupo HF em 60 dias em rela????o ao grupo H e redu????o no grupo HFTC em rela????o ao grupo HF no mesmo tempo (H: 0,18???0,02; HF: 0,46???0,04; HFTC 0,35???0,03 ??m/mg de prote??na). No balan??o redox, o grupo aos grupos H e HF apresentaram redu????o da raz??o GSH/GSSG em 7, 15, 30 e 60 dias em rela????o aos grupos HFTC. Nossos resultados evidenciam que com apenas 7 de consumo de frutose houve preju??zo no controle auton??mico da circula????o, que foi seguida por redu????o de nitritos, aumento de IL-6 e TNF-??? no cora????o em 15 dias, culminando em aumento de les??o ?? prote??nas e lip??deos nesse tecido, que provavelmente se associam ao aparecimento das disfun????es cardiometab??licas a partir de 30 dias de protocolo nos SHR. Adicionalmente, o treinamento f??sico combinado impediu o desenvolvimento da disfun????o auton??mica neste modelo, o que provavelmente promoveu altera????es neuroimunes e de perfil de estresse oxidativo favor??veis, culminado em marcante atenua????o das disfun????es cardiometab??licas em SHR submetidos ao consumo de frutose. Em conjunto nossos achados refor??am o papel do sistema nervoso aut??nomo na g??nese das disfun????es cardiometab??licas e evidenciam um importante papel do treinamento f??sico combinado na preven????o dessas altera????es.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-07-16T20:49:37Z No. of bitstreams: 1 Danielle da Silva Dias.pdf: 3090914 bytes, checksum: d346273638612b707bd90d03e883db70 (MD5)Made available in DSpace on 2018-07-16T20:49:37Z (GMT). No. of bitstreams: 1 Danielle da Silva Dias.pdf: 3090914 bytes, checksum: d346273638612b707bd90d03e883db70 (MD5) Previous issue date: 2017-10-19application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??demodula????o auton??mica cardiovascularconsumo de frutosetreinamento f??sico combinadoinflama????oestresse oxidativocardiovascular autonomic modulationfructose consumptioncombined exercise traininginflammationoxidative stressCIENCIAS DA SAUDEImpacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensosImpact of combined exercise training on development of neuroimmune and cardiovascular dysfunctions induced by frutose overload in hypertensive ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALDanielle da Silva Dias.pdfDanielle da Silva Dias.pdfapplication/pdf3090914http://localhost:8080/tede/bitstream/tede/1836/2/Danielle+da+Silva+Dias.pdfd346273638612b707bd90d03e883db70MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1836/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18362018-07-16 17:49:37.184oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-07-16T20:49:37Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos |
dc.title.alternative.eng.fl_str_mv |
Impact of combined exercise training on development of neuroimmune and cardiovascular dysfunctions induced by frutose overload in hypertensive rats |
title |
Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos |
spellingShingle |
Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos Dias, Danielle da Silva modula????o auton??mica cardiovascular consumo de frutose treinamento f??sico combinado inflama????o estresse oxidativo cardiovascular autonomic modulation fructose consumption combined exercise training inflammation oxidative stress CIENCIAS DA SAUDE |
title_short |
Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos |
title_full |
Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos |
title_fullStr |
Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos |
title_full_unstemmed |
Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos |
title_sort |
Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos |
author |
Dias, Danielle da Silva |
author_facet |
Dias, Danielle da Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Angelis, K??tia de |
dc.contributor.referee1.fl_str_mv |
Angelis, K??tia de |
dc.contributor.referee2.fl_str_mv |
Trombetta, Ivani Credidio |
dc.contributor.referee3.fl_str_mv |
Corso, Simone Dal |
dc.contributor.referee4.fl_str_mv |
Irigoyen, Maria Claudia |
dc.contributor.referee5.fl_str_mv |
Rodrigues, Bruno |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6644420445148028 |
dc.contributor.author.fl_str_mv |
Dias, Danielle da Silva |
contributor_str_mv |
Angelis, K??tia de Angelis, K??tia de Trombetta, Ivani Credidio Corso, Simone Dal Irigoyen, Maria Claudia Rodrigues, Bruno |
dc.subject.por.fl_str_mv |
modula????o auton??mica cardiovascular consumo de frutose treinamento f??sico combinado inflama????o estresse oxidativo |
topic |
modula????o auton??mica cardiovascular consumo de frutose treinamento f??sico combinado inflama????o estresse oxidativo cardiovascular autonomic modulation fructose consumption combined exercise training inflammation oxidative stress CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
cardiovascular autonomic modulation fructose consumption combined exercise training inflammation oxidative stress |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The aim of the present study was to evaluate the impact of combined exercise training on the development of cardiovascular and neuroimmune dysfunctions induced by fructose overload in sedentary hypertensive (SH), SHR + fructose (HF) and SHR + fructose + training (Treadmill + ladder, 60 days, 40-60% of maximal capacity) (HFTC). The groups were divided into subgroups evaluated at 7, 15, 30 and 60 days (n=6/group/time). Fructose was offered in drinking water (10%). Metabolic, hemodynamic, autonomic, inflammatory, and oxidative stress parameters were evaluated. Regarding the metabolic profile, the HF group showed increase in white adipose tissue in relation to the H group and the HFTC group diminished these values values at 60 days (HF: 1.91?? 0.10 vs. H: 1.61??0.11 and HFTC: 1.42??0.13 g). The HF group presented reduction in insulin sensitivity (HF: 3.15??0.2 vs. H: 3.96??0,1 and HFTC: 4.32??0.2 %/min) at 60 days in relation to H and HFTC groups. The HF group increased triglycerides (TG) when compared to H group at 60 days of protocol (HF:139??7 vs. H: 106??5 and HFTC:107??8 mg/dL). TG were lower in the HFTC group than in the HF group at 60 days. Fructose consumption (HF) induced a further increase in mean arterial pressure (MAP) at 30 and 60 days in SHR (HF-30 days: 153???4 e HF-60 days: 184??4 vs. H-30 days: 141???3 e H-60 days: 165???3 mmHg). On the other hand, combined exercise training reduced MAP in 30 and 60 days of fructose overload in relation to the HF group (HFTC: 142??8 and 167??6 mmHg). There was a decrease on heart rate variability (VAR-PI) in 7 days and 60 days in the HF group (10.5??1.3 and 40.81?? 6.12 ms2) in relation to the H group (23??1.5 and 59.7 ??3.4 ms2); the HFTC group did not presented this impairment (26.8??2.1 and 70.4??5.9 ms2). In addition, the HF group had a lower alpha index (spontaneous baroreflex) at 7 days compared to the H group (0.23??0.03 vs. 0.35??0.01 ms/mmHg), which was not observed in the HFTC group. There was an increase in IL-6 and TNF?? in cardiac tissue at 15, 30 and 60 days in the HF and HFTC groups compared to the H groups. The HFTC group presented higher IL-10 values in the heart at 7 days compared to the HF group (28??1 vs. 16??1 pg/mg of protein). The HF group presented increase in cardiac lipoperoxidation at 30 and 60 days in relation to the H group. The HFTC group decreased lipoperoxidation compared to HF group at 60 days (1302??58 vs. 1956??215 cps/mg protein) and there was also reduction in protein oxidation in this time. In addition, there was an increase in NADPH oxidase in the HF group at 60 days when compared to the H group and reduction in this parameter in the HFTC group in relation to the HF group at the same time (H: 0.18??0.02; HF: 0.46 ??0.04; HFTC 0.35 ??0.03 ??m/mg protein). Considering the redox balance, the HFTC group presented increase in this evaluation in 7, 15, 30 and 60 days in relation to H and HF groups. Our results show that only 7 days of fructose consumption impaired the autonomic control of the circulation, followed by reduction in plasma nitrites and increase in cardiac IL-6 and TNF-??? in 15 days, culminating in increased cardiac protein and lipids damage, which are probably associated with the appearance of cardiometabolic dysfunctions after 30 days of protocol in SHR. In addition, combined exercise training prevented the development of autonomic dysfunction in this model, which probably promoted favorable neuroimmune changes and oxidative stress profile, culminating in a marked attenuation of cardiometabolic dysfunctions in SHR submitted to fructose consumption. Together our findings reinforce the role of the autonomic nervous system in the genesis of cardiometabolic dysfunctions and evidence an important role of combined exercise training in the prevention of these alterations. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-10-19 |
dc.date.accessioned.fl_str_mv |
2018-07-16T20:49:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Dias, Danielle da Silva. Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos. 2017. 171 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1836 |
identifier_str_mv |
Dias, Danielle da Silva. Impacto do treinamento f??sico combinado no desenvolvimento das disfun????es cardiovasculares e neuroimunes induzidas pela sobrecarga de frutose em ratos hipertensos. 2017. 171 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1836 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
8765449414823306929 |
dc.relation.confidence.fl_str_mv |
600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
dc.publisher.initials.fl_str_mv |
UNINOVE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Sa??de |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.source.none.fl_str_mv |
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Universidade Nove de Julho (UNINOVE) |
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UNINOVE |
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UNINOVE |
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Biblioteca Digital de Teses e Dissertações da Uninove |
collection |
Biblioteca Digital de Teses e Dissertações da Uninove |
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Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
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bibliotecatede@uninove.br||bibliotecatede@uninove.br |
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