Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Nutrição |
Texto Completo: | https://periodicos.puc-campinas.edu.br/nutricao/article/view/9502 |
Resumo: | ObjectiveThe aim of this study was to assess the effect of whey protein consumption on the lipid, hepatic and renal metabolism, bone formation and antioxidant defense system of rats given a hypercholesterolemic diet. MethodsA total of 32 adult Fisher rats were divided into 4 groups: C group, standard diet; H group, hypercholesterolemic diet; WP group, standard diet with whey protein; WPH group, hypercholesterolemic diet with whey protein. Food ingestion and weight gain were monitored weekly. After 8 weeks, blood was collected for biochemical analysis and the rats were killed. The statistical tests analysis of variance and Tukey’s test were performed. ResultsWhey protein did not lower serum cholesterol significantly and increased serum triglycerides in hypercholesterolemic rats. However, whey protein decreased the activity of aspartate aminotransferase and alkaline phosphatase, as well as serum creatinine. The assessed antioxidant defenses were not affected. Diets containing whey protein generated heavier, thicker and longer bones than casein diets. ConclusionWhey proteins did not have a significant hypocholesterolemic effect in rats. On the other hand, they significantly prevented changes in liver and kidney functions brought about by hypercholesterolemic diets. Data also suggest that whey proteins benefit bone formation when compared with casein diets. |
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Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic ratsInfluência das proteínas do soro sobre enzimas hepáticas, perfil lipídico e formação óssea de ratos hipercolesterolêmicosAmino AcidsAspartate aminotransferasesAlkaline phosphataseCholesterolHypercholesterolemiaPeptides and proteinsAminoácidosAspartato aminotransferasesColesterolFosfatase alcalinaHipercolesterolemiaPeptídeos e proteínasObjectiveThe aim of this study was to assess the effect of whey protein consumption on the lipid, hepatic and renal metabolism, bone formation and antioxidant defense system of rats given a hypercholesterolemic diet. MethodsA total of 32 adult Fisher rats were divided into 4 groups: C group, standard diet; H group, hypercholesterolemic diet; WP group, standard diet with whey protein; WPH group, hypercholesterolemic diet with whey protein. Food ingestion and weight gain were monitored weekly. After 8 weeks, blood was collected for biochemical analysis and the rats were killed. The statistical tests analysis of variance and Tukey’s test were performed. ResultsWhey protein did not lower serum cholesterol significantly and increased serum triglycerides in hypercholesterolemic rats. However, whey protein decreased the activity of aspartate aminotransferase and alkaline phosphatase, as well as serum creatinine. The assessed antioxidant defenses were not affected. Diets containing whey protein generated heavier, thicker and longer bones than casein diets. ConclusionWhey proteins did not have a significant hypocholesterolemic effect in rats. On the other hand, they significantly prevented changes in liver and kidney functions brought about by hypercholesterolemic diets. Data also suggest that whey proteins benefit bone formation when compared with casein diets. ObjetivoAvaliar o efeito do consumo das proteínas do soro do leite sobre parâmetros do metabolismo lipídico, hepático e renal, a formação óssea e as defesas antioxidantes de ratos submetidos à dieta hipercolesterolemiante. MétodosTrinta e dois ratos Fisher adultos foram divididos em 4 grupos, recebendo as seguintes dietas: grupo C, dieta padrão; grupo H, dieta hipercolesterolemiante; grupo PS, dieta padrão e proteínas do soro; grupo PSH dieta hipercolesterolemiante e proteínas do soro. A ingestão alimentar e o ganho de peso foram monitorados semanalmente. Após 8 semanas, o sangue dos animais foi coletado para dosagem bioquímica e os animais foram eutanasiados. Foram utilizados os testes estatísticos, análise de variância e o teste de Tukey. ResultadosAs proteínas do soro não reduziram o colesterol plasmático de forma significante, e promoveram um aumento na concentração plasmática de triacilgliceróis nos animais hipercolesterolêmicos. No entanto, reduziram a atividade da aspartato aminotransferase e da fosfatase alcalina, assim como a concentração plasmática de creatinina. Não se observou nenhum efeito sobre as defesas antioxidantes avaliadas. As dietas contendo as proteínas do soro geraram ossos mais pesados, com maior diâmetro e comprimento que as dietas contendo caseína. ConclusãoAs proteínas do soro não apresentaram um efeito hipocolesterolemiante significante em ratos. No entanto, impediram de forma significante a ocorrência de alterações nos parâmetros indicadores das funções hepáticas e renais provocadas pela dieta hipercolesterolemiante. Os dados sugerem também que as proteínas do soro afetam positivamente a formação óssea, quando comparados com as dietas contendo caseína como fonte protéica.Núcleo de Editoração – PUC-Campinas2023-08-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9502Brazilian Journal of Nutrition; Vol. 22 No. 4 (2009): Revista de NutriçãoRevista de Nutrição; Vol. 22 Núm. 4 (2009): Revista de NutriçãoRevista de Nutrição; v. 22 n. 4 (2009): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9502/6868Copyright (c) 2023 Fabiano Kenji HARAGUCHI, Maria Lucia PEDROSA, Heberth de PAULA, Rinaldo Cardoso dos SANTOS, Marcelo Eustáquio SILVAhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Kenji HARAGUCHI, Fabiano PEDROSA, Maria Lucia de PAULA, HeberthCardoso dos SANTOS, Rinaldo Eustáquio SILVA, Marcelo 2023-08-31T12:31:57Zoai:ojs.periodicos.puc-campinas.edu.br:article/9502Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-08-31T12:31:57Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats Influência das proteínas do soro sobre enzimas hepáticas, perfil lipídico e formação óssea de ratos hipercolesterolêmicos |
title |
Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats |
spellingShingle |
Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats Kenji HARAGUCHI, Fabiano Amino Acids Aspartate aminotransferases Alkaline phosphatase Cholesterol Hypercholesterolemia Peptides and proteins Aminoácidos Aspartato aminotransferases Colesterol Fosfatase alcalina Hipercolesterolemia Peptídeos e proteínas |
title_short |
Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats |
title_full |
Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats |
title_fullStr |
Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats |
title_full_unstemmed |
Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats |
title_sort |
Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats |
author |
Kenji HARAGUCHI, Fabiano |
author_facet |
Kenji HARAGUCHI, Fabiano PEDROSA, Maria Lucia de PAULA, Heberth Cardoso dos SANTOS, Rinaldo Eustáquio SILVA, Marcelo |
author_role |
author |
author2 |
PEDROSA, Maria Lucia de PAULA, Heberth Cardoso dos SANTOS, Rinaldo Eustáquio SILVA, Marcelo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Kenji HARAGUCHI, Fabiano PEDROSA, Maria Lucia de PAULA, Heberth Cardoso dos SANTOS, Rinaldo Eustáquio SILVA, Marcelo |
dc.subject.por.fl_str_mv |
Amino Acids Aspartate aminotransferases Alkaline phosphatase Cholesterol Hypercholesterolemia Peptides and proteins Aminoácidos Aspartato aminotransferases Colesterol Fosfatase alcalina Hipercolesterolemia Peptídeos e proteínas |
topic |
Amino Acids Aspartate aminotransferases Alkaline phosphatase Cholesterol Hypercholesterolemia Peptides and proteins Aminoácidos Aspartato aminotransferases Colesterol Fosfatase alcalina Hipercolesterolemia Peptídeos e proteínas |
description |
ObjectiveThe aim of this study was to assess the effect of whey protein consumption on the lipid, hepatic and renal metabolism, bone formation and antioxidant defense system of rats given a hypercholesterolemic diet. MethodsA total of 32 adult Fisher rats were divided into 4 groups: C group, standard diet; H group, hypercholesterolemic diet; WP group, standard diet with whey protein; WPH group, hypercholesterolemic diet with whey protein. Food ingestion and weight gain were monitored weekly. After 8 weeks, blood was collected for biochemical analysis and the rats were killed. The statistical tests analysis of variance and Tukey’s test were performed. ResultsWhey protein did not lower serum cholesterol significantly and increased serum triglycerides in hypercholesterolemic rats. However, whey protein decreased the activity of aspartate aminotransferase and alkaline phosphatase, as well as serum creatinine. The assessed antioxidant defenses were not affected. Diets containing whey protein generated heavier, thicker and longer bones than casein diets. ConclusionWhey proteins did not have a significant hypocholesterolemic effect in rats. On the other hand, they significantly prevented changes in liver and kidney functions brought about by hypercholesterolemic diets. Data also suggest that whey proteins benefit bone formation when compared with casein diets. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9502 |
url |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9502 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9502/6868 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Nutrition; Vol. 22 No. 4 (2009): Revista de Nutrição Revista de Nutrição; Vol. 22 Núm. 4 (2009): Revista de Nutrição Revista de Nutrição; v. 22 n. 4 (2009): Revista de Nutrição 1678-9865 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
||sbi.submissionrn@puc-campinas.edu.br |
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1799126073721487360 |