Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Fisioterapia em Movimento |
Texto Completo: | https://periodicos.pucpr.br/fisio/article/view/28106 |
Resumo: | Introduction: In the rehabilitation of musculoskeletal injuries, ultrasound is widely used in clinical practice. Objective: To evaluate the effects of pulsed ultrasonic therapy on the viability and modulation of genes involved in inflammation (IL-6) and neovascularization (VEGF) processes of L929 fibroblast cells. Methods: For irradiation with ultrasound the cells were subdivided into groups: G1 (without irradiation), G2 (0.3 W/cm2-20%) and G3 (0.6 W/cm2-20%), with periods of treatment at 24, 48 and 72 hours. The cell viability assay was analyzed by the MTT method and gene modulation was analyzed by RT-qPCR method. Results: After the comparative analysis between groups, only G2 and G3 (48-hour) presented statistically significant differences in relation to the control. In relation to the gene expression, the selection of the groups analyzed was delimited according to the comparative analysis of the values obtained by the MTT test. Thus, after the achievement of RT-qPCR, it can be observed that in G2, the amount of VEGF gene transcripts increased by 1.125-fold compared to endogenous controls and, in G3, increased 1.388-fold. Already, the IL-6 gene had its transcripts reduced in both G2 (5.64x10-9)and G3 (1.91x10-6). Conclusion: Pulsed ultrasound in L929 fibroblasts showed a significant biostimulatory effect in the 48-hour period, with increased cell viability, and the same effect in the modulation of gene expression related the neovascularization and Inflammation, mediating the acceleration of the tissue repair cascade. |
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PUC_PR-26 |
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Fisioterapia em Movimento |
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|
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Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblastsRehabilitation. Fibroblasts. Gene expression. Regeneration. Ultrasonic therapy.Introduction: In the rehabilitation of musculoskeletal injuries, ultrasound is widely used in clinical practice. Objective: To evaluate the effects of pulsed ultrasonic therapy on the viability and modulation of genes involved in inflammation (IL-6) and neovascularization (VEGF) processes of L929 fibroblast cells. Methods: For irradiation with ultrasound the cells were subdivided into groups: G1 (without irradiation), G2 (0.3 W/cm2-20%) and G3 (0.6 W/cm2-20%), with periods of treatment at 24, 48 and 72 hours. The cell viability assay was analyzed by the MTT method and gene modulation was analyzed by RT-qPCR method. Results: After the comparative analysis between groups, only G2 and G3 (48-hour) presented statistically significant differences in relation to the control. In relation to the gene expression, the selection of the groups analyzed was delimited according to the comparative analysis of the values obtained by the MTT test. Thus, after the achievement of RT-qPCR, it can be observed that in G2, the amount of VEGF gene transcripts increased by 1.125-fold compared to endogenous controls and, in G3, increased 1.388-fold. Already, the IL-6 gene had its transcripts reduced in both G2 (5.64x10-9)and G3 (1.91x10-6). Conclusion: Pulsed ultrasound in L929 fibroblasts showed a significant biostimulatory effect in the 48-hour period, with increased cell viability, and the same effect in the modulation of gene expression related the neovascularization and Inflammation, mediating the acceleration of the tissue repair cascade.Editora PUCPRESS2021-01-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.pucpr.br/fisio/article/view/2810610.1590/fm.2021.34112Fisioterapia em Movimento (Physical Therapy in Movement); Vol. 34 (2021): n. cont.Fisioterapia em Movimento; v. 34 (2021): n. cont.1980-5918reponame:Fisioterapia em Movimentoinstname:Pontifícia Universidade Católica do Paraná (PUC-PR)instacron:PUC_PRenghttps://periodicos.pucpr.br/fisio/article/view/28106/pdfCopyright (c) 2022 PUCPRESSinfo:eu-repo/semantics/openAccessPerrucini, Priscila Daniele de OliveiraFranco de Oliveira, RodrigoPires, Flavia BeltrãoBertin, Larissa DragonettiPires-Oliveira, Deise Aparecida de AlmeidaFrederico, Regina Célia Poli2022-03-07T19:02:55Zoai:ojs.periodicos.pucpr.br:article/28106Revistahttps://periodicos.pucpr.br/fisioPRIhttps://periodicos.pucpr.br/fisio/oairubia.farias@pucpr.br||revista.fisioterapia@pucpr.br1980-59180103-5150opendoar:2022-03-07T19:02:55Fisioterapia em Movimento - Pontifícia Universidade Católica do Paraná (PUC-PR)false |
dc.title.none.fl_str_mv |
Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts |
title |
Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts |
spellingShingle |
Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts Perrucini, Priscila Daniele de Oliveira Rehabilitation. Fibroblasts. Gene expression. Regeneration. Ultrasonic therapy. |
title_short |
Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts |
title_full |
Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts |
title_fullStr |
Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts |
title_full_unstemmed |
Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts |
title_sort |
Ultrasonic therapy modulates the expression of genes related to neovascularization and inflammation in fibroblasts |
author |
Perrucini, Priscila Daniele de Oliveira |
author_facet |
Perrucini, Priscila Daniele de Oliveira Franco de Oliveira, Rodrigo Pires, Flavia Beltrão Bertin, Larissa Dragonetti Pires-Oliveira, Deise Aparecida de Almeida Frederico, Regina Célia Poli |
author_role |
author |
author2 |
Franco de Oliveira, Rodrigo Pires, Flavia Beltrão Bertin, Larissa Dragonetti Pires-Oliveira, Deise Aparecida de Almeida Frederico, Regina Célia Poli |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Perrucini, Priscila Daniele de Oliveira Franco de Oliveira, Rodrigo Pires, Flavia Beltrão Bertin, Larissa Dragonetti Pires-Oliveira, Deise Aparecida de Almeida Frederico, Regina Célia Poli |
dc.subject.por.fl_str_mv |
Rehabilitation. Fibroblasts. Gene expression. Regeneration. Ultrasonic therapy. |
topic |
Rehabilitation. Fibroblasts. Gene expression. Regeneration. Ultrasonic therapy. |
description |
Introduction: In the rehabilitation of musculoskeletal injuries, ultrasound is widely used in clinical practice. Objective: To evaluate the effects of pulsed ultrasonic therapy on the viability and modulation of genes involved in inflammation (IL-6) and neovascularization (VEGF) processes of L929 fibroblast cells. Methods: For irradiation with ultrasound the cells were subdivided into groups: G1 (without irradiation), G2 (0.3 W/cm2-20%) and G3 (0.6 W/cm2-20%), with periods of treatment at 24, 48 and 72 hours. The cell viability assay was analyzed by the MTT method and gene modulation was analyzed by RT-qPCR method. Results: After the comparative analysis between groups, only G2 and G3 (48-hour) presented statistically significant differences in relation to the control. In relation to the gene expression, the selection of the groups analyzed was delimited according to the comparative analysis of the values obtained by the MTT test. Thus, after the achievement of RT-qPCR, it can be observed that in G2, the amount of VEGF gene transcripts increased by 1.125-fold compared to endogenous controls and, in G3, increased 1.388-fold. Already, the IL-6 gene had its transcripts reduced in both G2 (5.64x10-9)and G3 (1.91x10-6). Conclusion: Pulsed ultrasound in L929 fibroblasts showed a significant biostimulatory effect in the 48-hour period, with increased cell viability, and the same effect in the modulation of gene expression related the neovascularization and Inflammation, mediating the acceleration of the tissue repair cascade. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.pucpr.br/fisio/article/view/28106 10.1590/fm.2021.34112 |
url |
https://periodicos.pucpr.br/fisio/article/view/28106 |
identifier_str_mv |
10.1590/fm.2021.34112 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.pucpr.br/fisio/article/view/28106/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 PUCPRESS info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 PUCPRESS |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora PUCPRESS |
publisher.none.fl_str_mv |
Editora PUCPRESS |
dc.source.none.fl_str_mv |
Fisioterapia em Movimento (Physical Therapy in Movement); Vol. 34 (2021): n. cont. Fisioterapia em Movimento; v. 34 (2021): n. cont. 1980-5918 reponame:Fisioterapia em Movimento instname:Pontifícia Universidade Católica do Paraná (PUC-PR) instacron:PUC_PR |
instname_str |
Pontifícia Universidade Católica do Paraná (PUC-PR) |
instacron_str |
PUC_PR |
institution |
PUC_PR |
reponame_str |
Fisioterapia em Movimento |
collection |
Fisioterapia em Movimento |
repository.name.fl_str_mv |
Fisioterapia em Movimento - Pontifícia Universidade Católica do Paraná (PUC-PR) |
repository.mail.fl_str_mv |
rubia.farias@pucpr.br||revista.fisioterapia@pucpr.br |
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1799138749489086464 |