Hypothyroidism alters salivary secretion and composition in septic rats

Detalhes bibliográficos
Autor(a) principal: Lima, Flávia Oliveira de
Data de Publicação: 2008
Outros Autores: Ramalho, Maria José Pedreira, Rummler, Maria do Carmo Oliveira, Rodriguez, Tania Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3747
Resumo: Purpose: We investigated the effects of hypothyroidism on salivary flow rate, total protein and electrolytes concentrations (Na+, K+ and Ca+2) in septic rats. Methods: Male Wistar rats (200-280g) were thyroidectomized (TX) or sham operated (N) and treated with lypopolysaccharide (LPS) (250μg/100g b.w.; i.p.) or saline (SL), 90 min before salivary stimulation with pilocarpine (5mg/kg; i.p.). Saliva was collected over 15 min (μL/min/100g of b.w.). Total proteins were analyzed by Lorwy method and electrolytes concentrations were determined by flame fotometry (Na+, K+) and colorimetric test (Ca+2). Results: Hypothyroidism and sepsis by LPS injection caused a decrease in salivary flow rates (Mann-Whitney Test, P < 0.05). LPS injection decreased salivary total protein concentration in TX rats and sodium concentration in N rats. Sepsis and thyroid hormones do not participate in the molecular mechanism of potassium transport, but hypothyroidism increased calcium concentration in saliva. Conclusion: Salivary flow rate and composition are modified by hypothyroidism in rats with sepsis. Key words: Saliva; hypothyroidism; sepsis
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spelling Hypothyroidism alters salivary secretion and composition in septic ratsHipotireoidismo altera secreção e composição salivar em ratos sépticosSalivahypothyroidismsepsisSalivaPurpose: We investigated the effects of hypothyroidism on salivary flow rate, total protein and electrolytes concentrations (Na+, K+ and Ca+2) in septic rats. Methods: Male Wistar rats (200-280g) were thyroidectomized (TX) or sham operated (N) and treated with lypopolysaccharide (LPS) (250μg/100g b.w.; i.p.) or saline (SL), 90 min before salivary stimulation with pilocarpine (5mg/kg; i.p.). Saliva was collected over 15 min (μL/min/100g of b.w.). Total proteins were analyzed by Lorwy method and electrolytes concentrations were determined by flame fotometry (Na+, K+) and colorimetric test (Ca+2). Results: Hypothyroidism and sepsis by LPS injection caused a decrease in salivary flow rates (Mann-Whitney Test, P < 0.05). LPS injection decreased salivary total protein concentration in TX rats and sodium concentration in N rats. Sepsis and thyroid hormones do not participate in the molecular mechanism of potassium transport, but hypothyroidism increased calcium concentration in saliva. Conclusion: Salivary flow rate and composition are modified by hypothyroidism in rats with sepsis. Key words: Saliva; hypothyroidism; sepsisObjetivo: Este estudo investigou os efeitos do hipotireoidismo na secreção salivar total, nas concentrações de proteína e de eletrólitos (Na+, K+ e Ca+2) da saliva estimulada de ratos sépticos. Metodologia: Foram utilizados ratos Wistar (200-280g) separados em dois grupos: tireoidectomizados (TX) e controle (N). Os ratos receberam injeção intraperitoneal de lipopolissacarídeo (LPS), 250μg/100g de peso corporal (p.c.), ou salina (SL), 90 min antes da estimulação salivar com pilocarpina (5mg/kg de p.c.) para coleta de fluxo salivar total por 15 min (μL/min/100g de p.c.). A concentração de proteínas totais foi analisada pelo método de Lorwy e as concentrações de eletrólitos através de reagente padrão (Doles) para fotômetro de chama (Na+, K+) e teste colorimétrico (Ca+2). Resultados: O hipotireoidismo e a endotoxemia induzida pelo LPS diminuíram o fluxo salivar (Teste de Mann-Whitney, P < 0,05). A sepse reduziu significativamente a concentração de proteínas totais salivar de ratos TX e a concentração de íon sódio na saliva de ratos N. Não houve alteração nas concentrações de íon potássio em nenhum dos grupos, mas o hipotireoidismo provocou aumento da concentração de íon cálcio na saliva. Conclusão: A secreção salivar e a composição da saliva são alteradas pelo hipotireoidismo em ratos sépticos. Palavras-chave: Saliva; hipotireoidismo; sepseEDIPUCRS - Editora Universitária da PUCRS2008-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleanimal model; experimental; prospectiveapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3747Revista Odonto Ciência; Vol. 23 No. 1 (2008); 53 - 57Revista Odonto Ciência; v. 23 n. 1 (2008); 53 - 571980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3747/2873Lima, Flávia Oliveira deRamalho, Maria José PedreiraRummler, Maria do Carmo OliveiraRodriguez, Tania Tavaresinfo:eu-repo/semantics/openAccess2013-11-18T10:55:40Zoai:ojs.revistaseletronicas.pucrs.br:article/3747Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-18T10:55:40Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Hypothyroidism alters salivary secretion and composition in septic rats
Hipotireoidismo altera secreção e composição salivar em ratos sépticos
title Hypothyroidism alters salivary secretion and composition in septic rats
spellingShingle Hypothyroidism alters salivary secretion and composition in septic rats
Lima, Flávia Oliveira de
Saliva
hypothyroidism
sepsis
Saliva
title_short Hypothyroidism alters salivary secretion and composition in septic rats
title_full Hypothyroidism alters salivary secretion and composition in septic rats
title_fullStr Hypothyroidism alters salivary secretion and composition in septic rats
title_full_unstemmed Hypothyroidism alters salivary secretion and composition in septic rats
title_sort Hypothyroidism alters salivary secretion and composition in septic rats
author Lima, Flávia Oliveira de
author_facet Lima, Flávia Oliveira de
Ramalho, Maria José Pedreira
Rummler, Maria do Carmo Oliveira
Rodriguez, Tania Tavares
author_role author
author2 Ramalho, Maria José Pedreira
Rummler, Maria do Carmo Oliveira
Rodriguez, Tania Tavares
author2_role author
author
author
dc.contributor.author.fl_str_mv Lima, Flávia Oliveira de
Ramalho, Maria José Pedreira
Rummler, Maria do Carmo Oliveira
Rodriguez, Tania Tavares
dc.subject.por.fl_str_mv Saliva
hypothyroidism
sepsis
Saliva
topic Saliva
hypothyroidism
sepsis
Saliva
description Purpose: We investigated the effects of hypothyroidism on salivary flow rate, total protein and electrolytes concentrations (Na+, K+ and Ca+2) in septic rats. Methods: Male Wistar rats (200-280g) were thyroidectomized (TX) or sham operated (N) and treated with lypopolysaccharide (LPS) (250μg/100g b.w.; i.p.) or saline (SL), 90 min before salivary stimulation with pilocarpine (5mg/kg; i.p.). Saliva was collected over 15 min (μL/min/100g of b.w.). Total proteins were analyzed by Lorwy method and electrolytes concentrations were determined by flame fotometry (Na+, K+) and colorimetric test (Ca+2). Results: Hypothyroidism and sepsis by LPS injection caused a decrease in salivary flow rates (Mann-Whitney Test, P < 0.05). LPS injection decreased salivary total protein concentration in TX rats and sodium concentration in N rats. Sepsis and thyroid hormones do not participate in the molecular mechanism of potassium transport, but hypothyroidism increased calcium concentration in saliva. Conclusion: Salivary flow rate and composition are modified by hypothyroidism in rats with sepsis. Key words: Saliva; hypothyroidism; sepsis
publishDate 2008
dc.date.none.fl_str_mv 2008-07-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
animal model; experimental; prospective
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3747
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3747
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3747/2873
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 23 No. 1 (2008); 53 - 57
Revista Odonto Ciência; v. 23 n. 1 (2008); 53 - 57
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
_version_ 1754820874329915392

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