Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English]
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Scientia Medica (Porto Alegre. Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/scientiamedica/article/view/3837 |
Resumo: | Aims: To develop a technique to recover, count, identify, and measure chorionic villi and other chorionic structures present in the peripheral blood of pregnant women. Methods: Ten normal pregnant women with gestational age equal or over 37 weeks, admitted to the Hospital São Lucas da PUCRS for delivery, were selected for the study. Three milliliters of blood samples in EDTA and 5,000 IU of aprotinine (Trasylol® Bayer) were obtained at a cubital vein from each patient. Samples were immediately fixed in Bouin, embedded in paraffin, sectioned at 3 μm, stained by hematoxylin/eosin, Masson’s trichrome, and with monoclonal antibodies specific to trophoblastic tissues. Each slide was scrutinized over all its surface at 250x magnification, by a linear scrutiny with conventional microscopy and every chorionic structure recognized was counted and measured with a calibrated micrometric ocular. The measures have been presented by mean ± standard error. Results: For each milliliter of maternal blood, 15.4 ± 3.1 syncytiotrophoblastic plaques equal or larger than 100 μm and 5.8 ± 0,9 chorionic villi have been recovered. The mean longitudinal diameter of villi was 289.3 ± 13.6 μm, their mean transverse diameter was 116.3 ± 5.3 μm and their syncytiotrophoblast thickness was 33.4 ± 1.7 μm. In two of the six samples subjected to immunologic studies, a reactivity to monoclonal antibodies specific to trophoblast was obtained on the recovered structures. Conclusions: Immediate fixation of blood samples was presented as a new method to recover chorionic structures from peripheral blood of pregnant women. They have been identified, counted and measured. Their quantity and size were large. The mechanisms that could explain their presence and fate in maternal blood and the significance of their presence, quantity and fate have been discussed. KEY WORDS: PREGNANCY/blood; PREGNANCY/immunology; CHORIONIC VILLI; TROPHOBLASTS; HUMANS. |
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Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English]Vilosidades e outras estruturas coriônicas encontradas no sangue periférico de gestantes: número, tamanho e uma técnica para sua recuperaçãogravidez humanasangue maternodeportação trofoblásticavilosidades coriônicasAims: To develop a technique to recover, count, identify, and measure chorionic villi and other chorionic structures present in the peripheral blood of pregnant women. Methods: Ten normal pregnant women with gestational age equal or over 37 weeks, admitted to the Hospital São Lucas da PUCRS for delivery, were selected for the study. Three milliliters of blood samples in EDTA and 5,000 IU of aprotinine (Trasylol® Bayer) were obtained at a cubital vein from each patient. Samples were immediately fixed in Bouin, embedded in paraffin, sectioned at 3 μm, stained by hematoxylin/eosin, Masson’s trichrome, and with monoclonal antibodies specific to trophoblastic tissues. Each slide was scrutinized over all its surface at 250x magnification, by a linear scrutiny with conventional microscopy and every chorionic structure recognized was counted and measured with a calibrated micrometric ocular. The measures have been presented by mean ± standard error. Results: For each milliliter of maternal blood, 15.4 ± 3.1 syncytiotrophoblastic plaques equal or larger than 100 μm and 5.8 ± 0,9 chorionic villi have been recovered. The mean longitudinal diameter of villi was 289.3 ± 13.6 μm, their mean transverse diameter was 116.3 ± 5.3 μm and their syncytiotrophoblast thickness was 33.4 ± 1.7 μm. In two of the six samples subjected to immunologic studies, a reactivity to monoclonal antibodies specific to trophoblast was obtained on the recovered structures. Conclusions: Immediate fixation of blood samples was presented as a new method to recover chorionic structures from peripheral blood of pregnant women. They have been identified, counted and measured. Their quantity and size were large. The mechanisms that could explain their presence and fate in maternal blood and the significance of their presence, quantity and fate have been discussed. KEY WORDS: PREGNANCY/blood; PREGNANCY/immunology; CHORIONIC VILLI; TROPHOBLASTS; HUMANS.Objetivos: desenvolver uma técnica para recuperar, identificar, contar e medir as vilosidades e outras estruturas coriônicas presentes no sangue periférico de gestantes. Métodos: foram selecionadas para o estudo 10 gestantes normais, com idade gestacional igual ou superior a 37 semanas, internadas no Hospital São Lucas da PUCRS para o parto. Foram colhidos 3 ml de sangue em EDTA e 5.000 UI de aprotinina (Trasylol® Bayer) de uma veia cubital de cada paciente. As amostras foram imediatamente fixadas em Bouin, embebidas em parafina, cortadas a 3μm, coradas por hematoxilina/eosina, tricrômico de Masson e por anticorpos monoclonais específicos para tecidos trofoblásticos. Cada lâmina foi escrutinada de forma linear em toda a sua superfície, a um aumento de 250 vezes, e as estruturas coriônicas reconhecidas foram medidas com uma ocular micrométrica calibrada. Os resultados foram apresentados por média ± erro padrão. Resultados: foram recuperadas 15,4 ± 3,1 placas de sinciciotrofoblasto maiores de 100 e 5,8 ± 0,9 vilosidades coriônicas por ml de sangue materno. O comprimento médio das vilosidades foi 289,3 ± 13, 6 μm, a largura média foi de 116,3 ± 5,3 μm e a espessura média do sinciciotrofoblasto foi de 33,4 ± 1,7 μm. O descolamento dos cortes impediu os estudos imunológicos em 4 dos 10 casos. Em duas das seis amostras estudadas com anticorpos monoclonais específicos para trofoblasto as estruturas recuperadas foram reativas. Conclusões: a fixação imediata das amostras foi apresentada como um novo método para recuperar as estruturas coriônicas presentes no sangue periférico de gestantes. Elas foram identificadas, contadas e medidas. Sua quantidade e dimensões foram grandes. Os mecanismos que podem explicar seu surgimento e seu destino, bem como o significado de sua presença e quantidade no sangue periférico das gestantes foram discutidos. DESCRITORES: GRAVIDEZ/sangue; GRAVIDEZ/imunologia; VILOSIDADES CORIÔNICAS; TROFOBLASTOS; HUMANOS.Editora da PUCRS - ediPUCRS2008-08-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionMedicinaapplication/pdfapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/3837Scientia Medica; Vol. 18 No. 3 (2008); 110-118Scientia Medica; v. 18 n. 3 (2008); 110-1181980-61081806-5562reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSporenghttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/3837/3273https://revistaseletronicas.pucrs.br/scientiamedica/article/view/3837/7831Luz, Nilo PereiraMarques, Caio Coelhoinfo:eu-repo/semantics/openAccess2013-07-17T13:17:33Zoai:ojs.revistaseletronicas.pucrs.br:article/3837Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2013-07-17T13:17:33Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English] Vilosidades e outras estruturas coriônicas encontradas no sangue periférico de gestantes: número, tamanho e uma técnica para sua recuperação |
title |
Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English] |
spellingShingle |
Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English] Luz, Nilo Pereira gravidez humana sangue materno deportação trofoblástica vilosidades coriônicas |
title_short |
Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English] |
title_full |
Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English] |
title_fullStr |
Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English] |
title_full_unstemmed |
Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English] |
title_sort |
Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery [Abstract in English] |
author |
Luz, Nilo Pereira |
author_facet |
Luz, Nilo Pereira Marques, Caio Coelho |
author_role |
author |
author2 |
Marques, Caio Coelho |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Luz, Nilo Pereira Marques, Caio Coelho |
dc.subject.por.fl_str_mv |
gravidez humana sangue materno deportação trofoblástica vilosidades coriônicas |
topic |
gravidez humana sangue materno deportação trofoblástica vilosidades coriônicas |
description |
Aims: To develop a technique to recover, count, identify, and measure chorionic villi and other chorionic structures present in the peripheral blood of pregnant women. Methods: Ten normal pregnant women with gestational age equal or over 37 weeks, admitted to the Hospital São Lucas da PUCRS for delivery, were selected for the study. Three milliliters of blood samples in EDTA and 5,000 IU of aprotinine (Trasylol® Bayer) were obtained at a cubital vein from each patient. Samples were immediately fixed in Bouin, embedded in paraffin, sectioned at 3 μm, stained by hematoxylin/eosin, Masson’s trichrome, and with monoclonal antibodies specific to trophoblastic tissues. Each slide was scrutinized over all its surface at 250x magnification, by a linear scrutiny with conventional microscopy and every chorionic structure recognized was counted and measured with a calibrated micrometric ocular. The measures have been presented by mean ± standard error. Results: For each milliliter of maternal blood, 15.4 ± 3.1 syncytiotrophoblastic plaques equal or larger than 100 μm and 5.8 ± 0,9 chorionic villi have been recovered. The mean longitudinal diameter of villi was 289.3 ± 13.6 μm, their mean transverse diameter was 116.3 ± 5.3 μm and their syncytiotrophoblast thickness was 33.4 ± 1.7 μm. In two of the six samples subjected to immunologic studies, a reactivity to monoclonal antibodies specific to trophoblast was obtained on the recovered structures. Conclusions: Immediate fixation of blood samples was presented as a new method to recover chorionic structures from peripheral blood of pregnant women. They have been identified, counted and measured. Their quantity and size were large. The mechanisms that could explain their presence and fate in maternal blood and the significance of their presence, quantity and fate have been discussed. KEY WORDS: PREGNANCY/blood; PREGNANCY/immunology; CHORIONIC VILLI; TROPHOBLASTS; HUMANS. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-08-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Medicina |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/3837 |
url |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/3837 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/3837/3273 https://revistaseletronicas.pucrs.br/scientiamedica/article/view/3837/7831 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Editora da PUCRS - ediPUCRS |
publisher.none.fl_str_mv |
Editora da PUCRS - ediPUCRS |
dc.source.none.fl_str_mv |
Scientia Medica; Vol. 18 No. 3 (2008); 110-118 Scientia Medica; v. 18 n. 3 (2008); 110-118 1980-6108 1806-5562 reponame:Scientia Medica (Porto Alegre. Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Scientia Medica (Porto Alegre. Online) |
collection |
Scientia Medica (Porto Alegre. Online) |
repository.name.fl_str_mv |
Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
scientiamedica@pucrs.br || editora.periodicos@pucrs.br |
_version_ |
1809101748920385536 |