Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease

Detalhes bibliográficos
Autor(a) principal: Marote, Ana Maria Franco Aveiro
Data de Publicação: 2018
Outros Autores: Pomeshchik, Yuriy, Goldwurm, Stefano, Collin, Anna, Lamas, Nuno J., Pinto, Luísa, Salgado, A. J., Roybon, Laurent
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/57878
Resumo: An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of common pluripotency markers, in vitro differentiation into three germ layers and normal karyotype. This iPSC line can be used to study the mechanisms underlying the development of PD.
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spelling Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's diseaseAdultCell DifferentiationCell LineCellular ReprogrammingHumansInduced Pluripotent Stem CellsKruppel-Like Transcription FactorsMaleOctamer Transcription Factor-3Parkinson DiseaseProto-Oncogene Proteins c-mycSOXB1 Transcription FactorsCiências Médicas::Medicina BásicaScience & TechnologyAn induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of common pluripotency markers, in vitro differentiation into three germ layers and normal karyotype. This iPSC line can be used to study the mechanisms underlying the development of PD.‘Cell Line and DNA Biobank from Patients affected by Genetic Diseases’ (Istituto G. Gaslini, Genova, Italy) and the Parkinson Institute Biobank, members of the Telethon Network of Genetic Biobanks (http://biobanknetwork.telethon.it; project no. GTB12001) funded by Telethon Italy, for providing fibroblasts samples. This work was supported by the Strategic Research Environment MultiPark at Lund University, the strong research environment BAGADILICO (grant 349-2007-8626), the Swedish Parkinson Foundation (Parkinsonfonden, grant 889/16), the Swedish Research Council (grant 2015-03684 to LR) and Finnish Cultural Foundation (grant 00161167 to YP). We also acknowledge the Portuguese Foundation for Science and Technologyinfo:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoMarote, Ana Maria Franco AveiroPomeshchik, YuriyGoldwurm, StefanoCollin, AnnaLamas, Nuno J.Pinto, LuísaSalgado, A. J.Roybon, Laurent20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/57878engMarote, A., Pomeshchik, Y., Goldwurm, et. al. (2018). Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease. Stem cell research, 27, 82-851873-50611876-775310.1016/j.scr.2018.01.00729334630https://www.sciencedirect.com/science/article/pii/S1873506118300072info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:27Zoai:repositorium.sdum.uminho.pt:1822/57878Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:56.450324Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease
title Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease
spellingShingle Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease
Marote, Ana Maria Franco Aveiro
Adult
Cell Differentiation
Cell Line
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells
Kruppel-Like Transcription Factors
Male
Octamer Transcription Factor-3
Parkinson Disease
Proto-Oncogene Proteins c-myc
SOXB1 Transcription Factors
Ciências Médicas::Medicina Básica
Science & Technology
title_short Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease
title_full Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease
title_fullStr Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease
title_full_unstemmed Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease
title_sort Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease
author Marote, Ana Maria Franco Aveiro
author_facet Marote, Ana Maria Franco Aveiro
Pomeshchik, Yuriy
Goldwurm, Stefano
Collin, Anna
Lamas, Nuno J.
Pinto, Luísa
Salgado, A. J.
Roybon, Laurent
author_role author
author2 Pomeshchik, Yuriy
Goldwurm, Stefano
Collin, Anna
Lamas, Nuno J.
Pinto, Luísa
Salgado, A. J.
Roybon, Laurent
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Marote, Ana Maria Franco Aveiro
Pomeshchik, Yuriy
Goldwurm, Stefano
Collin, Anna
Lamas, Nuno J.
Pinto, Luísa
Salgado, A. J.
Roybon, Laurent
dc.subject.por.fl_str_mv Adult
Cell Differentiation
Cell Line
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells
Kruppel-Like Transcription Factors
Male
Octamer Transcription Factor-3
Parkinson Disease
Proto-Oncogene Proteins c-myc
SOXB1 Transcription Factors
Ciências Médicas::Medicina Básica
Science & Technology
topic Adult
Cell Differentiation
Cell Line
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells
Kruppel-Like Transcription Factors
Male
Octamer Transcription Factor-3
Parkinson Disease
Proto-Oncogene Proteins c-myc
SOXB1 Transcription Factors
Ciências Médicas::Medicina Básica
Science & Technology
description An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of common pluripotency markers, in vitro differentiation into three germ layers and normal karyotype. This iPSC line can be used to study the mechanisms underlying the development of PD.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/57878
url http://hdl.handle.net/1822/57878
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Marote, A., Pomeshchik, Y., Goldwurm, et. al. (2018). Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease. Stem cell research, 27, 82-85
1873-5061
1876-7753
10.1016/j.scr.2018.01.007
29334630
https://www.sciencedirect.com/science/article/pii/S1873506118300072
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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