Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents

Detalhes bibliográficos
Autor(a) principal: Alves, Celso
Data de Publicação: 2020
Outros Autores: Serrano, Eurico, Silva, Joana, Rodrigues, Carlos, Pinteus, Susete, Gaspar, Helena, Botana, Luís M., Alpoim, Maria C., Pedrosa, Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/5931
Resumo: Acknowledgments This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/ 2020 and UID/Multi/04046/2020 granted to MARE—Marine and Environmental Sciences Centre and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery project (PTDC/ MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145- FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 - LISBOA-01-0145-FEDER-016405) and through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD "Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (reference Centro-01- 0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/ BD/103255/2014).
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spelling Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agentsMarine natural productsRed seaweedLung cancerCancer stem cellsMicroenvironment Interleukin-6Acknowledgments This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/ 2020 and UID/Multi/04046/2020 granted to MARE—Marine and Environmental Sciences Centre and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery project (PTDC/ MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145- FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 - LISBOA-01-0145-FEDER-016405) and through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD "Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (reference Centro-01- 0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/ BD/103255/2014).Cancer is one of the major threats to human health and, due to distinct factors, it is expected that its incidence will increase in the next decades leading to an urgent need of new anticancer drugs development. Ongoing experimental and clinical observations propose that cancer cells with stem-like properties (CSCs) are involved on the development of lung cancer chemoresistance. As tumour growth and metastasis can be controlled by tumourassociated stromal cells, the main goal of this study was to access the antitumor potential of five bromoterpenes isolated from Sphaerococcus coronopifolius red alga to target CSCs originated in a co-culture system of fibroblast and lung malignant cells. Cytotoxicity of compounds (10–500 μM; 72 h) was evaluated on monocultures of several malignant and non-malignant cells lines (HBF, BEAS-2B, RenG2, SC-DRenG2) and the effects estimated by MTT assay. Co-cultures of non-malignant human bronchial fibroblasts (HBF) and malignant human bronchial epithelial cells (RenG2) were implemented and the compounds ability to selectively kill CSCs was evaluated by sphere forming assay. The interleucine-6 (IL-6) levels were also determined as cytokine is crucial for CSCs. Regarding the monocultures results bromosphaerol selectively eliminated the malignant cells. Both 12S-hydroxy-bromosphaerol and 12R-hydroxy-bromosphaerol steroisomers were cytotoxic towards non-malignant bronchial BEAS-2B cell line, IC50 of 4.29 and 4.30 μM respectively. However, none of the steroisomers induced damage in the HBFs. As to the co-cultures, 12R-hydroxy-bromosphaerol revealed the highest cytotoxicity and ability to abrogate the malignant stem cells; however its effects were IL-6 independent. The results presented here are the first evidence of the potential of these bromoterpenes to abrogate CSCs opening new research opportunities. The 12R-hydroxy-bromosphaerol revealed to be the most promising compound to be test in more complex living models.ElsevierIC-OnlineAlves, CelsoSerrano, EuricoSilva, JoanaRodrigues, CarlosPinteus, SuseteGaspar, HelenaBotana, Luís M.Alpoim, Maria C.Pedrosa, Rui2021-07-23T14:32:26Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/5931engAlves, C., Serrano, E., Silva, J., Rodrigues, C., Pinteus, S., Gaspar, H., Botana, L.M., Alpoim, M.C. & Pedrosa, R. (2020). Biomedicine & Pharmacotherapy, 128. https://doi.org/10.1016/j.biopha.2020.1102750753-332210.1016/j.biopha.2020.110275info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:52:01Zoai:iconline.ipleiria.pt:10400.8/5931Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:49:17.507945Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents
title Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents
spellingShingle Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents
Alves, Celso
Marine natural products
Red seaweed
Lung cancer
Cancer stem cells
Microenvironment Interleukin-6
title_short Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents
title_full Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents
title_fullStr Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents
title_full_unstemmed Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents
title_sort Sphaerococcus coronopifolius bromoterpenes as potential cancer stem celltargeting agents
author Alves, Celso
author_facet Alves, Celso
Serrano, Eurico
Silva, Joana
Rodrigues, Carlos
Pinteus, Susete
Gaspar, Helena
Botana, Luís M.
Alpoim, Maria C.
Pedrosa, Rui
author_role author
author2 Serrano, Eurico
Silva, Joana
Rodrigues, Carlos
Pinteus, Susete
Gaspar, Helena
Botana, Luís M.
Alpoim, Maria C.
Pedrosa, Rui
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Alves, Celso
Serrano, Eurico
Silva, Joana
Rodrigues, Carlos
Pinteus, Susete
Gaspar, Helena
Botana, Luís M.
Alpoim, Maria C.
Pedrosa, Rui
dc.subject.por.fl_str_mv Marine natural products
Red seaweed
Lung cancer
Cancer stem cells
Microenvironment Interleukin-6
topic Marine natural products
Red seaweed
Lung cancer
Cancer stem cells
Microenvironment Interleukin-6
description Acknowledgments This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/ 2020 and UID/Multi/04046/2020 granted to MARE—Marine and Environmental Sciences Centre and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery project (PTDC/ MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145- FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 - LISBOA-01-0145-FEDER-016405) and through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD "Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (reference Centro-01- 0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/ BD/103255/2014).
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-07-23T14:32:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/5931
url http://hdl.handle.net/10400.8/5931
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, C., Serrano, E., Silva, J., Rodrigues, C., Pinteus, S., Gaspar, H., Botana, L.M., Alpoim, M.C. & Pedrosa, R. (2020). Biomedicine & Pharmacotherapy, 128. https://doi.org/10.1016/j.biopha.2020.110275
0753-3322
10.1016/j.biopha.2020.110275
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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