Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4645 |
Resumo: | Skin injuries are traumatic events, which are seldom accompanied by complete structural and functional restoration of the original tissue. Different strategies have been developed in order to make the wound healing process faster and less painful. In the present study in vitro and in vivo assays were carried out to evaluate the applicability of a dextran hydrogel loaded with chitosan microparticles containing epidermal and vascular endothelial growth factors, for the improvement of the wound healing process. The carriers' morphology was characterized by scanning electron microscopy. Their cytotoxicity profile and degradation by-products were evaluated through in vitro assays. In vivo experiments were also performed to evaluate their applicability for the treatment of skin burns. The wound healing process was monitored through macroscopic and histological analysis. The macroscopic analysis showed that the period for wound healing occurs in animals treated with microparticle loaded hydrogels containing growth factors that were considerably smaller than that of control groups. Moreover, the histological analysis revealed the absence of reactive or granulomatous inflammatory reaction in skin lesions. The results obtained both in vitro and in vivo disclosed that these systems and its degradation by-products are biocompatible, contributed to the re-establishment of skin architecture and can be used in a near future for the controlled delivery of other bioactive agents used in regenerative medicine. |
id |
RCAP_0686647698c1d38a5aae8958ebf4ea67 |
---|---|
oai_identifier_str |
oai:ubibliorum.ubi.pt:10400.6/4645 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healingWound healingDrug delivery systemsBiocompatibilityGrowth factorsIn vivo and in vitro studiesSkin injuries are traumatic events, which are seldom accompanied by complete structural and functional restoration of the original tissue. Different strategies have been developed in order to make the wound healing process faster and less painful. In the present study in vitro and in vivo assays were carried out to evaluate the applicability of a dextran hydrogel loaded with chitosan microparticles containing epidermal and vascular endothelial growth factors, for the improvement of the wound healing process. The carriers' morphology was characterized by scanning electron microscopy. Their cytotoxicity profile and degradation by-products were evaluated through in vitro assays. In vivo experiments were also performed to evaluate their applicability for the treatment of skin burns. The wound healing process was monitored through macroscopic and histological analysis. The macroscopic analysis showed that the period for wound healing occurs in animals treated with microparticle loaded hydrogels containing growth factors that were considerably smaller than that of control groups. Moreover, the histological analysis revealed the absence of reactive or granulomatous inflammatory reaction in skin lesions. The results obtained both in vitro and in vivo disclosed that these systems and its degradation by-products are biocompatible, contributed to the re-establishment of skin architecture and can be used in a near future for the controlled delivery of other bioactive agents used in regenerative medicine.ElsevieruBibliorumRibeiro, MP.Morgado, Patrícia I.Miguel, Sónia P.Coutinho, Paula Isabel Teixeira GonçalvesCorreia, Ilídio Joaquim Sobreira2018-03-20T09:50:53Z2013-03-242013-03-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4645engRibeiro, M.P., Morgado, P.I., Miguel, S.P., Coutinho, P. e Correia, I.J. (2013) "Dextran based-hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing", Materials Science & Engineering C - Materials for Biological Applications, Vol. 33(5), pp. 2958-6610.1016/j.msec.2013.03.025metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T02:31:43Zoai:ubibliorum.ubi.pt:10400.6/4645Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:42.360641Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing |
title |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing |
spellingShingle |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing Ribeiro, MP. Wound healing Drug delivery systems Biocompatibility Growth factors In vivo and in vitro studies |
title_short |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing |
title_full |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing |
title_fullStr |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing |
title_full_unstemmed |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing |
title_sort |
Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing |
author |
Ribeiro, MP. |
author_facet |
Ribeiro, MP. Morgado, Patrícia I. Miguel, Sónia P. Coutinho, Paula Isabel Teixeira Gonçalves Correia, Ilídio Joaquim Sobreira |
author_role |
author |
author2 |
Morgado, Patrícia I. Miguel, Sónia P. Coutinho, Paula Isabel Teixeira Gonçalves Correia, Ilídio Joaquim Sobreira |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Ribeiro, MP. Morgado, Patrícia I. Miguel, Sónia P. Coutinho, Paula Isabel Teixeira Gonçalves Correia, Ilídio Joaquim Sobreira |
dc.subject.por.fl_str_mv |
Wound healing Drug delivery systems Biocompatibility Growth factors In vivo and in vitro studies |
topic |
Wound healing Drug delivery systems Biocompatibility Growth factors In vivo and in vitro studies |
description |
Skin injuries are traumatic events, which are seldom accompanied by complete structural and functional restoration of the original tissue. Different strategies have been developed in order to make the wound healing process faster and less painful. In the present study in vitro and in vivo assays were carried out to evaluate the applicability of a dextran hydrogel loaded with chitosan microparticles containing epidermal and vascular endothelial growth factors, for the improvement of the wound healing process. The carriers' morphology was characterized by scanning electron microscopy. Their cytotoxicity profile and degradation by-products were evaluated through in vitro assays. In vivo experiments were also performed to evaluate their applicability for the treatment of skin burns. The wound healing process was monitored through macroscopic and histological analysis. The macroscopic analysis showed that the period for wound healing occurs in animals treated with microparticle loaded hydrogels containing growth factors that were considerably smaller than that of control groups. Moreover, the histological analysis revealed the absence of reactive or granulomatous inflammatory reaction in skin lesions. The results obtained both in vitro and in vivo disclosed that these systems and its degradation by-products are biocompatible, contributed to the re-establishment of skin architecture and can be used in a near future for the controlled delivery of other bioactive agents used in regenerative medicine. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-24 2013-03-24T00:00:00Z 2018-03-20T09:50:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4645 |
url |
http://hdl.handle.net/10400.6/4645 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro, M.P., Morgado, P.I., Miguel, S.P., Coutinho, P. e Correia, I.J. (2013) "Dextran based-hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing", Materials Science & Engineering C - Materials for Biological Applications, Vol. 33(5), pp. 2958-66 10.1016/j.msec.2013.03.025 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136354442936320 |