Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/910 |
Resumo: | Objective: Obesity is the result of positive energy balance. It can be caused by excessive energy consumption but also by decreased energy dissipation, which occurs under several conditions including when the development or activation of brown adipose tissue (BAT) is impaired. Here we evaluated whether iRhom2, the essential cofactor for the Tumour Necrosis Factor (TNF) sheddase ADAM17/TACE, plays a role in the pathophysiology of metabolic syndrome. Methods: We challenged WT versus iRhom2 KO mice to positive energy balance by chronic exposure to a high fat diet and then compared their metabolic phenotypes. We also carried out ex vivo assays with primary and immortalized mouse brown adipocytes to establish the autonomy of the effect of loss of iRhom2 on thermogenesis and respiration. Results: Deletion of iRhom2 protected mice from weight gain, dyslipidemia, adipose tissue inflammation, and hepatic steatosis and improved insulin sensitivity when challenged by a high fat diet. Crucially, the loss of iRhom2 promotes thermogenesis via BAT activation and beige adipocyte recruitment, enabling iRhom2 KO mice to dissipate excess energy more efficiently than WT animals. This effect on enhanced ther- mogenesis is cell-autonomous in brown adipocytes as iRhom2 KOs exhibit elevated UCP1 levels and increased mitochondrial proton leak. |
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7160 |
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Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesisiRhom2; ADAM17/TACEObesityInsulin resistanceNAFLDBATBrowningThermogenesisUCP1Objective: Obesity is the result of positive energy balance. It can be caused by excessive energy consumption but also by decreased energy dissipation, which occurs under several conditions including when the development or activation of brown adipose tissue (BAT) is impaired. Here we evaluated whether iRhom2, the essential cofactor for the Tumour Necrosis Factor (TNF) sheddase ADAM17/TACE, plays a role in the pathophysiology of metabolic syndrome. Methods: We challenged WT versus iRhom2 KO mice to positive energy balance by chronic exposure to a high fat diet and then compared their metabolic phenotypes. We also carried out ex vivo assays with primary and immortalized mouse brown adipocytes to establish the autonomy of the effect of loss of iRhom2 on thermogenesis and respiration. Results: Deletion of iRhom2 protected mice from weight gain, dyslipidemia, adipose tissue inflammation, and hepatic steatosis and improved insulin sensitivity when challenged by a high fat diet. Crucially, the loss of iRhom2 promotes thermogenesis via BAT activation and beige adipocyte recruitment, enabling iRhom2 KO mice to dissipate excess energy more efficiently than WT animals. This effect on enhanced ther- mogenesis is cell-autonomous in brown adipocytes as iRhom2 KOs exhibit elevated UCP1 levels and increased mitochondrial proton leak.Conclusion:Our data suggest that iRhom2 is a negative regulator of thermogenesis and plays a role in the control of adipose tissue homeostasis during metabolic disease.Elsevier GmbHARCABadenes, MarinaAmin, AbdulbasitGonzález-García, IsmaelFélix, InêsBurbridge, EmmaCavadas, MiguelOrtega, Francisco Joséde Carvalho, ÉrikaFaísca, PedroCarobbio, StefaniaSeixas, ElsaPedroso, DoraNeves-Costa, AnaMoita, Luís F.Fernández-Real, José ManuelVidal-Puig, AntónioDomingos, AnaLópez, MiguelAdrain, Colin2019-12-09T23:09:49Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/910eng10.1016/j.molmet.2019.10.006info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:19Zoai:arca.igc.gulbenkian.pt:10400.7/910Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:06.854967Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis |
title |
Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis |
spellingShingle |
Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis Badenes, Marina iRhom2; ADAM17/TACE Obesity Insulin resistance NAFLD BAT Browning Thermogenesis UCP1 |
title_short |
Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis |
title_full |
Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis |
title_fullStr |
Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis |
title_full_unstemmed |
Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis |
title_sort |
Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis |
author |
Badenes, Marina |
author_facet |
Badenes, Marina Amin, Abdulbasit González-García, Ismael Félix, Inês Burbridge, Emma Cavadas, Miguel Ortega, Francisco José de Carvalho, Érika Faísca, Pedro Carobbio, Stefania Seixas, Elsa Pedroso, Dora Neves-Costa, Ana Moita, Luís F. Fernández-Real, José Manuel Vidal-Puig, António Domingos, Ana López, Miguel Adrain, Colin |
author_role |
author |
author2 |
Amin, Abdulbasit González-García, Ismael Félix, Inês Burbridge, Emma Cavadas, Miguel Ortega, Francisco José de Carvalho, Érika Faísca, Pedro Carobbio, Stefania Seixas, Elsa Pedroso, Dora Neves-Costa, Ana Moita, Luís F. Fernández-Real, José Manuel Vidal-Puig, António Domingos, Ana López, Miguel Adrain, Colin |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Badenes, Marina Amin, Abdulbasit González-García, Ismael Félix, Inês Burbridge, Emma Cavadas, Miguel Ortega, Francisco José de Carvalho, Érika Faísca, Pedro Carobbio, Stefania Seixas, Elsa Pedroso, Dora Neves-Costa, Ana Moita, Luís F. Fernández-Real, José Manuel Vidal-Puig, António Domingos, Ana López, Miguel Adrain, Colin |
dc.subject.por.fl_str_mv |
iRhom2; ADAM17/TACE Obesity Insulin resistance NAFLD BAT Browning Thermogenesis UCP1 |
topic |
iRhom2; ADAM17/TACE Obesity Insulin resistance NAFLD BAT Browning Thermogenesis UCP1 |
description |
Objective: Obesity is the result of positive energy balance. It can be caused by excessive energy consumption but also by decreased energy dissipation, which occurs under several conditions including when the development or activation of brown adipose tissue (BAT) is impaired. Here we evaluated whether iRhom2, the essential cofactor for the Tumour Necrosis Factor (TNF) sheddase ADAM17/TACE, plays a role in the pathophysiology of metabolic syndrome. Methods: We challenged WT versus iRhom2 KO mice to positive energy balance by chronic exposure to a high fat diet and then compared their metabolic phenotypes. We also carried out ex vivo assays with primary and immortalized mouse brown adipocytes to establish the autonomy of the effect of loss of iRhom2 on thermogenesis and respiration. Results: Deletion of iRhom2 protected mice from weight gain, dyslipidemia, adipose tissue inflammation, and hepatic steatosis and improved insulin sensitivity when challenged by a high fat diet. Crucially, the loss of iRhom2 promotes thermogenesis via BAT activation and beige adipocyte recruitment, enabling iRhom2 KO mice to dissipate excess energy more efficiently than WT animals. This effect on enhanced ther- mogenesis is cell-autonomous in brown adipocytes as iRhom2 KOs exhibit elevated UCP1 levels and increased mitochondrial proton leak. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-09T23:09:49Z 2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/910 |
url |
http://hdl.handle.net/10400.7/910 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.molmet.2019.10.006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier GmbH |
publisher.none.fl_str_mv |
Elsevier GmbH |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130576573169664 |