Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/66084 |
Resumo: | Lignocellulosic materials require pretreatment to remove lignin enabling the enzyme access to the cellulose. This work used multivariate analysis to investigate the acid and alkali pretreatments of vine pruning followed by enzymatic hydrolysis. The best acid pretreatment conditions were H2SO4 1.5%, 120 °C for 30 min, removing 68.7% of hemicellulose, enabling 95.8% of cellulose recovery. However, this treatment was not enough to allow the enzyme hydrolysis. A second step of treatment with NaOH 3.0% at 120 °C without agitation for 60 min led to a material with 75.0% of cellulose and 25.0% of lignin. However, the lowest glucose yield (80.86% and 32.26 g L?1 of glucose) was obtained after the enzyme hydrolysis of this material. The highest glucose yield (98.72% with 35.06 g L?1) was obtained using a pretreated material containing 68.1% of cellulose and 31.9% of lignin obtained after a milder condition (NaOH 2% at 100 °C), thus showing that not all the lignin need to be removed to obtain a high saccharification yield. A less severe pretreatment with no adverse effect on the glucose yield with the advantage of preserving the non-cellulose biomass fractions was effective for vine prune valorization. |
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Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yieldCellulaseEnzyme hydrolysisVine pruningChemometricsScience & TechnologyLignocellulosic materials require pretreatment to remove lignin enabling the enzyme access to the cellulose. This work used multivariate analysis to investigate the acid and alkali pretreatments of vine pruning followed by enzymatic hydrolysis. The best acid pretreatment conditions were H2SO4 1.5%, 120 °C for 30 min, removing 68.7% of hemicellulose, enabling 95.8% of cellulose recovery. However, this treatment was not enough to allow the enzyme hydrolysis. A second step of treatment with NaOH 3.0% at 120 °C without agitation for 60 min led to a material with 75.0% of cellulose and 25.0% of lignin. However, the lowest glucose yield (80.86% and 32.26 g L?1 of glucose) was obtained after the enzyme hydrolysis of this material. The highest glucose yield (98.72% with 35.06 g L?1) was obtained using a pretreated material containing 68.1% of cellulose and 31.9% of lignin obtained after a milder condition (NaOH 2% at 100 °C), thus showing that not all the lignin need to be removed to obtain a high saccharification yield. A less severe pretreatment with no adverse effect on the glucose yield with the advantage of preserving the non-cellulose biomass fractions was effective for vine prune valorization.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, BioTecNorte operation (NORTE-01-0145-FEDER000004), the projects Multibiorefinery (POCI-01-0145-FEDER-016403), FoSynBio (POCI-01-0145-FEDER-029549) and Lignozymes (POCI-01- 0145-FEDER-029773) funded by the European Regional Development Fund under the scope of Norte 2020. In Brazil, this study was funded in part by the Coordenaçao ~ de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) - Finance Code 001, Conselho Nacional de Desenvolvimento Científico e Tecnologico (CNPq) and Fundaçao Cearense de Apoio ao Desenvolvimento Científico e Tecnologico (FUNCAP). The authors would like to acknowledge the Central Analytical (Physical Department) of Federal University of Ceara for conducting the SEM analysis and the Centro de Tecnologia Canavieira – CTC/Brazil for the support. E. Gudina and L. Rodrigues acknowledge FCT for the Post-doctoral (CEB-BPD/01/2015/07) and sabbatical (SFRH/BSAB/142991/2019) grants, respectively.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoFilho, Elenilson G. AlvesMaciel, Tatiane C.Gudiña, Eduardo JoséMiguel, Emilio C.Rodrigues, L. R.Rodrigues, Sueli2020-092020-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66084engFilho, Elenilson G. Alves; Maciel, Tatiane C.; Gudiña, Eduardo J.; Miguel, Emilio C.; Rodrigues, Lígia R.; Rodrigues, Sueli, Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield. Biomass & Bioenergy, 140(105653), 20200961-953410.1016/j.biombioe.2020.105653http://www.journals.elsevier.com/biomass-and-bioenergy/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:32Zoai:repositorium.sdum.uminho.pt:1822/66084Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:21.851104Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield |
title |
Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield |
spellingShingle |
Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield Filho, Elenilson G. Alves Cellulase Enzyme hydrolysis Vine pruning Chemometrics Science & Technology |
title_short |
Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield |
title_full |
Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield |
title_fullStr |
Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield |
title_full_unstemmed |
Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield |
title_sort |
Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield |
author |
Filho, Elenilson G. Alves |
author_facet |
Filho, Elenilson G. Alves Maciel, Tatiane C. Gudiña, Eduardo José Miguel, Emilio C. Rodrigues, L. R. Rodrigues, Sueli |
author_role |
author |
author2 |
Maciel, Tatiane C. Gudiña, Eduardo José Miguel, Emilio C. Rodrigues, L. R. Rodrigues, Sueli |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Filho, Elenilson G. Alves Maciel, Tatiane C. Gudiña, Eduardo José Miguel, Emilio C. Rodrigues, L. R. Rodrigues, Sueli |
dc.subject.por.fl_str_mv |
Cellulase Enzyme hydrolysis Vine pruning Chemometrics Science & Technology |
topic |
Cellulase Enzyme hydrolysis Vine pruning Chemometrics Science & Technology |
description |
Lignocellulosic materials require pretreatment to remove lignin enabling the enzyme access to the cellulose. This work used multivariate analysis to investigate the acid and alkali pretreatments of vine pruning followed by enzymatic hydrolysis. The best acid pretreatment conditions were H2SO4 1.5%, 120 °C for 30 min, removing 68.7% of hemicellulose, enabling 95.8% of cellulose recovery. However, this treatment was not enough to allow the enzyme hydrolysis. A second step of treatment with NaOH 3.0% at 120 °C without agitation for 60 min led to a material with 75.0% of cellulose and 25.0% of lignin. However, the lowest glucose yield (80.86% and 32.26 g L?1 of glucose) was obtained after the enzyme hydrolysis of this material. The highest glucose yield (98.72% with 35.06 g L?1) was obtained using a pretreated material containing 68.1% of cellulose and 31.9% of lignin obtained after a milder condition (NaOH 2% at 100 °C), thus showing that not all the lignin need to be removed to obtain a high saccharification yield. A less severe pretreatment with no adverse effect on the glucose yield with the advantage of preserving the non-cellulose biomass fractions was effective for vine prune valorization. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09 2020-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/66084 |
url |
http://hdl.handle.net/1822/66084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Filho, Elenilson G. Alves; Maciel, Tatiane C.; Gudiña, Eduardo J.; Miguel, Emilio C.; Rodrigues, Lígia R.; Rodrigues, Sueli, Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield. Biomass & Bioenergy, 140(105653), 2020 0961-9534 10.1016/j.biombioe.2020.105653 http://www.journals.elsevier.com/biomass-and-bioenergy/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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