Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/17992 |
Resumo: | The effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K) in soil was investigated, anchoring population and cellular level effects, i.e., survival, reproduction (28 days) and oxidative stress markers (0, 2, 4, 6, 10 days). The standard species Folsomia candida was used. Measured markers included catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST), total glutathione (TG), metallothionein (MT) and lipid peroxidation (LPO). Results showed that AgNO3 was more toxic than AgNPs at the population level: reproduction EC20 and EC50 was ca. 2 and 4 times lower, respectively. At the cellular level Correspondence Analysis showed a clear separation between AgNO3 and AgNP throughout time. Results showed differences in the mechanisms, indicating a combined effect of released Ag+ (MT and GST) and of AgNPs (CAT, GR, TG, LPO). Hence, clear advantages from mechanistic approaches are shown, but also that time is of importance when measuring such responses. |
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Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola)antioxidant defensesmechanisms of responsesoil invertebratesThe effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K) in soil was investigated, anchoring population and cellular level effects, i.e., survival, reproduction (28 days) and oxidative stress markers (0, 2, 4, 6, 10 days). The standard species Folsomia candida was used. Measured markers included catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST), total glutathione (TG), metallothionein (MT) and lipid peroxidation (LPO). Results showed that AgNO3 was more toxic than AgNPs at the population level: reproduction EC20 and EC50 was ca. 2 and 4 times lower, respectively. At the cellular level Correspondence Analysis showed a clear separation between AgNO3 and AgNP throughout time. Results showed differences in the mechanisms, indicating a combined effect of released Ag+ (MT and GST) and of AgNPs (CAT, GR, TG, LPO). Hence, clear advantages from mechanistic approaches are shown, but also that time is of importance when measuring such responses.MDPI2017-06-29T10:14:47Z2015-01-01T00:00:00Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/17992eng1660-460110.3390/ijerph121012530Mendes, Luís AndréMaria, Vera L.Scott-Fordsmand, Janeck J.Amorim, Mónica J. B.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:31:19Zoai:ria.ua.pt:10773/17992Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:51:49.097412Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola) |
title |
Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola) |
spellingShingle |
Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola) Mendes, Luís André antioxidant defenses mechanisms of response soil invertebrates |
title_short |
Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola) |
title_full |
Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola) |
title_fullStr |
Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola) |
title_full_unstemmed |
Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola) |
title_sort |
Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola) |
author |
Mendes, Luís André |
author_facet |
Mendes, Luís André Maria, Vera L. Scott-Fordsmand, Janeck J. Amorim, Mónica J. B. |
author_role |
author |
author2 |
Maria, Vera L. Scott-Fordsmand, Janeck J. Amorim, Mónica J. B. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mendes, Luís André Maria, Vera L. Scott-Fordsmand, Janeck J. Amorim, Mónica J. B. |
dc.subject.por.fl_str_mv |
antioxidant defenses mechanisms of response soil invertebrates |
topic |
antioxidant defenses mechanisms of response soil invertebrates |
description |
The effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K) in soil was investigated, anchoring population and cellular level effects, i.e., survival, reproduction (28 days) and oxidative stress markers (0, 2, 4, 6, 10 days). The standard species Folsomia candida was used. Measured markers included catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST), total glutathione (TG), metallothionein (MT) and lipid peroxidation (LPO). Results showed that AgNO3 was more toxic than AgNPs at the population level: reproduction EC20 and EC50 was ca. 2 and 4 times lower, respectively. At the cellular level Correspondence Analysis showed a clear separation between AgNO3 and AgNP throughout time. Results showed differences in the mechanisms, indicating a combined effect of released Ag+ (MT and GST) and of AgNPs (CAT, GR, TG, LPO). Hence, clear advantages from mechanistic approaches are shown, but also that time is of importance when measuring such responses. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01T00:00:00Z 2015 2017-06-29T10:14:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/17992 |
url |
http://hdl.handle.net/10773/17992 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1660-4601 10.3390/ijerph121012530 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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