Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/66347 |
Resumo: | We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) â a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion. |
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Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44BioactivityCD44Hyaluronic acidScience & TechnologyWe report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) â a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion.The authors acknowledge the financial support from the European Commission’s H2020 programme, under grant agreements H2020- WIDESPREAD-2014-668983-FORECAST and H2020-WIDESPREAD01-2016-2017-739572-THE DISCOVERIES CTR. S. A. acknowledges the Portuguese Foundation for Science and Technology (FCT) for her PhD grant (SFRH/BD/112075/2015)Royal Society of ChemistryUniversidade do MinhoAmorim, SaraPashkuleva, I.Reis, Celso A.Reis, R. L.Pires, R. A.2020-032020-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66347engAmorim S., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44, Journal Of Materials Chemistry B, Vol. 17, doi:10.1039/d0tb00407c, 20202050-750X2050-751810.1039/d0tb00407c32222753https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb00407c/unauth#!divAbstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:22Zoai:repositorium.sdum.uminho.pt:1822/66347Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:50.822173Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 |
title |
Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 |
spellingShingle |
Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 Amorim, Sara Bioactivity CD44 Hyaluronic acid Science & Technology |
title_short |
Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 |
title_full |
Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 |
title_fullStr |
Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 |
title_full_unstemmed |
Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 |
title_sort |
Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 |
author |
Amorim, Sara |
author_facet |
Amorim, Sara Pashkuleva, I. Reis, Celso A. Reis, R. L. Pires, R. A. |
author_role |
author |
author2 |
Pashkuleva, I. Reis, Celso A. Reis, R. L. Pires, R. A. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Amorim, Sara Pashkuleva, I. Reis, Celso A. Reis, R. L. Pires, R. A. |
dc.subject.por.fl_str_mv |
Bioactivity CD44 Hyaluronic acid Science & Technology |
topic |
Bioactivity CD44 Hyaluronic acid Science & Technology |
description |
We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) â a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03 2020-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/66347 |
url |
http://hdl.handle.net/1822/66347 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Amorim S., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44, Journal Of Materials Chemistry B, Vol. 17, doi:10.1039/d0tb00407c, 2020 2050-750X 2050-7518 10.1039/d0tb00407c 32222753 https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb00407c/unauth#!divAbstract |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132186104823808 |