Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/315 |
Resumo: | The actin-Capping Protein heterodimer, composed of the α and β subunits, is a master F-actin regulator. In addition to its role in many cellular processes, Capping Protein acts as a main tumor suppressor module in Drosophila and in humans, in part, by restricting the activity of Yorkie/YAP/TAZ oncogenes. We aimed in this report to understand how both subunits regulate each other in vivo. We show that the levels and capping activities of both subunits must be tightly regulated to control F-actin levels and consequently growth of the Drosophila wing. Overexpressing capping protein α and β decreases both F-actin levels and tissue growth, while expressing forms of Capping Protein that have dominant negative effects on F-actin promote tissue growth. Both subunits regulate each other's protein levels. In addition, overexpressing one of the subunit in tissues knocked-down for the other increases the mRNA and protein levels of the subunit knocked-down and compensates for its loss. We propose that the ability of the α and β subunits to control each other's levels assures that a pool of functional heterodimer is produced in sufficient quantities to restrict the development of tumor but not in excess to sustain normal tissue growth. |
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Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levelsProtein ExtractionDrosophilaRNAThe actin-Capping Protein heterodimer, composed of the α and β subunits, is a master F-actin regulator. In addition to its role in many cellular processes, Capping Protein acts as a main tumor suppressor module in Drosophila and in humans, in part, by restricting the activity of Yorkie/YAP/TAZ oncogenes. We aimed in this report to understand how both subunits regulate each other in vivo. We show that the levels and capping activities of both subunits must be tightly regulated to control F-actin levels and consequently growth of the Drosophila wing. Overexpressing capping protein α and β decreases both F-actin levels and tissue growth, while expressing forms of Capping Protein that have dominant negative effects on F-actin promote tissue growth. Both subunits regulate each other's protein levels. In addition, overexpressing one of the subunit in tissues knocked-down for the other increases the mRNA and protein levels of the subunit knocked-down and compensates for its loss. We propose that the ability of the α and β subunits to control each other's levels assures that a pool of functional heterodimer is produced in sufficient quantities to restrict the development of tumor but not in excess to sustain normal tissue growth.FCT- SFRH/BD/47261/2008PLOSARCAAmândio, Ana RitaGaspar, PedroWhited, Jessica LJanody, Florence2015-09-23T15:04:21Z2014-05-022014-05-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/315engAmaˆndio AR, Gaspar P, Whited JL, Janody F (2014) Subunits of the Drosophila Actin-Capping Protein Heterodimer Regulate Each Other at Multiple Levels. PLoS ONE 9(5): e96326. doi:10.1371/journal.pone.009632610.1371/journal.pone.0096326info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:43Zoai:arca.igc.gulbenkian.pt:10400.7/315Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:38.042879Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels |
title |
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels |
spellingShingle |
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels Amândio, Ana Rita Protein Extraction Drosophila RNA |
title_short |
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels |
title_full |
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels |
title_fullStr |
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels |
title_full_unstemmed |
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels |
title_sort |
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels |
author |
Amândio, Ana Rita |
author_facet |
Amândio, Ana Rita Gaspar, Pedro Whited, Jessica L Janody, Florence |
author_role |
author |
author2 |
Gaspar, Pedro Whited, Jessica L Janody, Florence |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Amândio, Ana Rita Gaspar, Pedro Whited, Jessica L Janody, Florence |
dc.subject.por.fl_str_mv |
Protein Extraction Drosophila RNA |
topic |
Protein Extraction Drosophila RNA |
description |
The actin-Capping Protein heterodimer, composed of the α and β subunits, is a master F-actin regulator. In addition to its role in many cellular processes, Capping Protein acts as a main tumor suppressor module in Drosophila and in humans, in part, by restricting the activity of Yorkie/YAP/TAZ oncogenes. We aimed in this report to understand how both subunits regulate each other in vivo. We show that the levels and capping activities of both subunits must be tightly regulated to control F-actin levels and consequently growth of the Drosophila wing. Overexpressing capping protein α and β decreases both F-actin levels and tissue growth, while expressing forms of Capping Protein that have dominant negative effects on F-actin promote tissue growth. Both subunits regulate each other's protein levels. In addition, overexpressing one of the subunit in tissues knocked-down for the other increases the mRNA and protein levels of the subunit knocked-down and compensates for its loss. We propose that the ability of the α and β subunits to control each other's levels assures that a pool of functional heterodimer is produced in sufficient quantities to restrict the development of tumor but not in excess to sustain normal tissue growth. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-02 2014-05-02T00:00:00Z 2015-09-23T15:04:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/315 |
url |
http://hdl.handle.net/10400.7/315 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Amaˆndio AR, Gaspar P, Whited JL, Janody F (2014) Subunits of the Drosophila Actin-Capping Protein Heterodimer Regulate Each Other at Multiple Levels. PLoS ONE 9(5): e96326. doi:10.1371/journal.pone.0096326 10.1371/journal.pone.0096326 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLOS |
publisher.none.fl_str_mv |
PLOS |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130572120915968 |