Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase

Detalhes bibliográficos
Autor(a) principal: Johnston, Esther M.
Data de Publicação: 2014
Outros Autores: Dell'Acqua, Simone, Ramos, Susana Jorge Guerreiro, Pauleta, Sofia Rocha, Moura, Isabel Maria Andrade Martins Galhardas de, Solomon, Edward I.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/133518
Resumo: This research was supported by NIH grant DK-31450 (E.I.S.) and National funds by FCT (Fundacao para a Ciencia e a Tecnologia) under the projects PEst-C/EQB/LA0006/2013 and PTDC/QUI-BIQ/116481/2010 (I.M.).
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spelling Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductaseCU-Z CLUSTERACHROMOBACTER-CYCLOCLASTESPSEUDOMONAS-NAUTICACATALYTIC CENTERN2O REDUCTIONPURIFICATIONENZYMERESONANCEMECHANISMINSIGHTThis research was supported by NIH grant DK-31450 (E.I.S.) and National funds by FCT (Fundacao para a Ciencia e a Tecnologia) under the projects PEst-C/EQB/LA0006/2013 and PTDC/QUI-BIQ/116481/2010 (I.M.).N2OR has been found to have two structural forms of its tetranuclear copper active site, the 4CuS Cu-Z* form and the 4Cu2S Cu-Z form. EPR, resonance Raman, and MCD spectroscopies have been used to determine the redox states of these sites under different reductant conditions, showing that the Cu-Z* site accesses the 1-hole and fully reduced redox states, while the Cu-Z site accesses the 2-hole and 1-hole redox states. Single-turnover reactions of N2OR for Cu-Z and Cu-Z* poised in these redox states and steady-state turnover assays with different proportions of Cu-Z and Cu-Z* show that only fully reduced Cu-Z* is catalytically competent in rapid turnover with N2O.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNJohnston, Esther M.Dell'Acqua, SimoneRamos, Susana Jorge GuerreiroPauleta, Sofia RochaMoura, Isabel Maria Andrade Martins Galhardas deSolomon, Edward I.2022-02-24T23:11:19Z2014-01-152014-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/133518eng0002-7863PURE: 164591https://doi.org/10.1021/ja411500pinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:12:15Zoai:run.unl.pt:10362/133518Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:47:50.129515Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
title Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
spellingShingle Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
Johnston, Esther M.
CU-Z CLUSTER
ACHROMOBACTER-CYCLOCLASTES
PSEUDOMONAS-NAUTICA
CATALYTIC CENTER
N2O REDUCTION
PURIFICATION
ENZYME
RESONANCE
MECHANISM
INSIGHT
title_short Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
title_full Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
title_fullStr Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
title_full_unstemmed Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
title_sort Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
author Johnston, Esther M.
author_facet Johnston, Esther M.
Dell'Acqua, Simone
Ramos, Susana Jorge Guerreiro
Pauleta, Sofia Rocha
Moura, Isabel Maria Andrade Martins Galhardas de
Solomon, Edward I.
author_role author
author2 Dell'Acqua, Simone
Ramos, Susana Jorge Guerreiro
Pauleta, Sofia Rocha
Moura, Isabel Maria Andrade Martins Galhardas de
Solomon, Edward I.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
RUN
dc.contributor.author.fl_str_mv Johnston, Esther M.
Dell'Acqua, Simone
Ramos, Susana Jorge Guerreiro
Pauleta, Sofia Rocha
Moura, Isabel Maria Andrade Martins Galhardas de
Solomon, Edward I.
dc.subject.por.fl_str_mv CU-Z CLUSTER
ACHROMOBACTER-CYCLOCLASTES
PSEUDOMONAS-NAUTICA
CATALYTIC CENTER
N2O REDUCTION
PURIFICATION
ENZYME
RESONANCE
MECHANISM
INSIGHT
topic CU-Z CLUSTER
ACHROMOBACTER-CYCLOCLASTES
PSEUDOMONAS-NAUTICA
CATALYTIC CENTER
N2O REDUCTION
PURIFICATION
ENZYME
RESONANCE
MECHANISM
INSIGHT
description This research was supported by NIH grant DK-31450 (E.I.S.) and National funds by FCT (Fundacao para a Ciencia e a Tecnologia) under the projects PEst-C/EQB/LA0006/2013 and PTDC/QUI-BIQ/116481/2010 (I.M.).
publishDate 2014
dc.date.none.fl_str_mv 2014-01-15
2014-01-15T00:00:00Z
2022-02-24T23:11:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/133518
url http://hdl.handle.net/10362/133518
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0002-7863
PURE: 164591
https://doi.org/10.1021/ja411500p
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138080814268416

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