Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/133518 |
Resumo: | This research was supported by NIH grant DK-31450 (E.I.S.) and National funds by FCT (Fundacao para a Ciencia e a Tecnologia) under the projects PEst-C/EQB/LA0006/2013 and PTDC/QUI-BIQ/116481/2010 (I.M.). |
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Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductaseCU-Z CLUSTERACHROMOBACTER-CYCLOCLASTESPSEUDOMONAS-NAUTICACATALYTIC CENTERN2O REDUCTIONPURIFICATIONENZYMERESONANCEMECHANISMINSIGHTThis research was supported by NIH grant DK-31450 (E.I.S.) and National funds by FCT (Fundacao para a Ciencia e a Tecnologia) under the projects PEst-C/EQB/LA0006/2013 and PTDC/QUI-BIQ/116481/2010 (I.M.).N2OR has been found to have two structural forms of its tetranuclear copper active site, the 4CuS Cu-Z* form and the 4Cu2S Cu-Z form. EPR, resonance Raman, and MCD spectroscopies have been used to determine the redox states of these sites under different reductant conditions, showing that the Cu-Z* site accesses the 1-hole and fully reduced redox states, while the Cu-Z site accesses the 2-hole and 1-hole redox states. Single-turnover reactions of N2OR for Cu-Z and Cu-Z* poised in these redox states and steady-state turnover assays with different proportions of Cu-Z and Cu-Z* show that only fully reduced Cu-Z* is catalytically competent in rapid turnover with N2O.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNJohnston, Esther M.Dell'Acqua, SimoneRamos, Susana Jorge GuerreiroPauleta, Sofia RochaMoura, Isabel Maria Andrade Martins Galhardas deSolomon, Edward I.2022-02-24T23:11:19Z2014-01-152014-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/133518eng0002-7863PURE: 164591https://doi.org/10.1021/ja411500pinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:12:15Zoai:run.unl.pt:10362/133518Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:47:50.129515Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase |
title |
Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase |
spellingShingle |
Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase Johnston, Esther M. CU-Z CLUSTER ACHROMOBACTER-CYCLOCLASTES PSEUDOMONAS-NAUTICA CATALYTIC CENTER N2O REDUCTION PURIFICATION ENZYME RESONANCE MECHANISM INSIGHT |
title_short |
Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase |
title_full |
Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase |
title_fullStr |
Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase |
title_full_unstemmed |
Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase |
title_sort |
Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase |
author |
Johnston, Esther M. |
author_facet |
Johnston, Esther M. Dell'Acqua, Simone Ramos, Susana Jorge Guerreiro Pauleta, Sofia Rocha Moura, Isabel Maria Andrade Martins Galhardas de Solomon, Edward I. |
author_role |
author |
author2 |
Dell'Acqua, Simone Ramos, Susana Jorge Guerreiro Pauleta, Sofia Rocha Moura, Isabel Maria Andrade Martins Galhardas de Solomon, Edward I. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
DQ - Departamento de Química CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE) RUN |
dc.contributor.author.fl_str_mv |
Johnston, Esther M. Dell'Acqua, Simone Ramos, Susana Jorge Guerreiro Pauleta, Sofia Rocha Moura, Isabel Maria Andrade Martins Galhardas de Solomon, Edward I. |
dc.subject.por.fl_str_mv |
CU-Z CLUSTER ACHROMOBACTER-CYCLOCLASTES PSEUDOMONAS-NAUTICA CATALYTIC CENTER N2O REDUCTION PURIFICATION ENZYME RESONANCE MECHANISM INSIGHT |
topic |
CU-Z CLUSTER ACHROMOBACTER-CYCLOCLASTES PSEUDOMONAS-NAUTICA CATALYTIC CENTER N2O REDUCTION PURIFICATION ENZYME RESONANCE MECHANISM INSIGHT |
description |
This research was supported by NIH grant DK-31450 (E.I.S.) and National funds by FCT (Fundacao para a Ciencia e a Tecnologia) under the projects PEst-C/EQB/LA0006/2013 and PTDC/QUI-BIQ/116481/2010 (I.M.). |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-15 2014-01-15T00:00:00Z 2022-02-24T23:11:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/133518 |
url |
http://hdl.handle.net/10362/133518 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0002-7863 PURE: 164591 https://doi.org/10.1021/ja411500p |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138080814268416 |