Amination of polymeric braid structures to improve tendon healing: An experimental comparison
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/87912 |
Resumo: | Several polymers are researched for tendon repair as polyethylene terephthalate (PET) and polylactic acid (PLA). These are biocompatible and useful in scaffolding repair though with minimal success due to long-term failure. There is a need to improve such scaffolds’ design and physical–chemical nature. This work concerns surface functionalization of polymeric braids (PET and PLA) that fulfill the high mechanical demands of tissues such as tendons. The functionalization aims to incorporate amine groups in the braids’ surface, improve cell adhesion, and consequently, the poor healing rate of these tissues and the biointegration of the braids. Two approaches are compared: the direct application of NH3 plasma and the surface grafting of EDA after O2 plasma activation. X-ray photoelectron spectroscopy (XPS) shows that amine groups are effectively introduced onto the samples’ surfaces. Besides, the plasma parameters chosen do not compromise the topography and tensile behavior of the braids. Resazurin assay and scanning electron microscopy show that the NH3 treatment improves cell–biomaterial interaction as improved cell adhesion and proliferation are observed. Both approaches are safe for biomedical applications. The NH3 plasma approach is more environmentally friendly, faster, and easier to scale-up, showing potential for application in the final hybrid medical device. |
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Amination of polymeric braid structures to improve tendon healing: An experimental comparisonEthylenediamineHybrid medical device (PET/PLA)NH3 plasmaSurface functionalizationTissue repairhybrid medical device (PETPLA)NH plasma 3Science & TechnologySeveral polymers are researched for tendon repair as polyethylene terephthalate (PET) and polylactic acid (PLA). These are biocompatible and useful in scaffolding repair though with minimal success due to long-term failure. There is a need to improve such scaffolds’ design and physical–chemical nature. This work concerns surface functionalization of polymeric braids (PET and PLA) that fulfill the high mechanical demands of tissues such as tendons. The functionalization aims to incorporate amine groups in the braids’ surface, improve cell adhesion, and consequently, the poor healing rate of these tissues and the biointegration of the braids. Two approaches are compared: the direct application of NH3 plasma and the surface grafting of EDA after O2 plasma activation. X-ray photoelectron spectroscopy (XPS) shows that amine groups are effectively introduced onto the samples’ surfaces. Besides, the plasma parameters chosen do not compromise the topography and tensile behavior of the braids. Resazurin assay and scanning electron microscopy show that the NH3 treatment improves cell–biomaterial interaction as improved cell adhesion and proliferation are observed. Both approaches are safe for biomedical applications. The NH3 plasma approach is more environmentally friendly, faster, and easier to scale-up, showing potential for application in the final hybrid medical device.T.P. and D.S. contributed equally to this work. Financial support from PT national funds (FCT/MCTES, Fundacao para a Ciencia e Tecnologia and Ministerio da Ciencia, Tecnologia e Ensino Superior) through the project UIDB/50006/2020 is acknowledged by REQUIMTE-LAQV authors. IPC authors acknowledge "National Funds through FCT-Portuguese Foundation for Science and Technology," References UIDB/05256/2020 and UIDP/05256/2020. T.P. acknowledges the financial support from FCT and ESF (European Social Fund) through North Portugal Regional Operational Program, through the PhD Grant PD/BD/143035/2018.WileyUniversidade do MinhoPeixoto, TâniaSilva, DanielRodrigues, MiguelNeto, MiguelSilva, RuiPaiva, Maria C.Grenho, LilianaFernandes, Maria HelenaLopes, Maria A.20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/87912engPeixoto, T., Silva, D., Rodrigues, M., Neto, M., Silva, R., Paiva, M. C., … Lopes, M. A. (2022, October 26). Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison. Macromolecular Materials and Engineering. Wiley. http://doi.org/10.1002/mame.2022004261438-749210.1002/mame.202200426https://onlinelibrary.wiley.com/doi/10.1002/mame.202200426info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-06T01:27:18Zoai:repositorium.sdum.uminho.pt:1822/87912Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:30:18.906566Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Amination of polymeric braid structures to improve tendon healing: An experimental comparison |
title |
Amination of polymeric braid structures to improve tendon healing: An experimental comparison |
spellingShingle |
Amination of polymeric braid structures to improve tendon healing: An experimental comparison Peixoto, Tânia Ethylenediamine Hybrid medical device (PET/PLA) NH3 plasma Surface functionalization Tissue repair hybrid medical device (PET PLA) NH plasma 3 Science & Technology |
title_short |
Amination of polymeric braid structures to improve tendon healing: An experimental comparison |
title_full |
Amination of polymeric braid structures to improve tendon healing: An experimental comparison |
title_fullStr |
Amination of polymeric braid structures to improve tendon healing: An experimental comparison |
title_full_unstemmed |
Amination of polymeric braid structures to improve tendon healing: An experimental comparison |
title_sort |
Amination of polymeric braid structures to improve tendon healing: An experimental comparison |
author |
Peixoto, Tânia |
author_facet |
Peixoto, Tânia Silva, Daniel Rodrigues, Miguel Neto, Miguel Silva, Rui Paiva, Maria C. Grenho, Liliana Fernandes, Maria Helena Lopes, Maria A. |
author_role |
author |
author2 |
Silva, Daniel Rodrigues, Miguel Neto, Miguel Silva, Rui Paiva, Maria C. Grenho, Liliana Fernandes, Maria Helena Lopes, Maria A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Peixoto, Tânia Silva, Daniel Rodrigues, Miguel Neto, Miguel Silva, Rui Paiva, Maria C. Grenho, Liliana Fernandes, Maria Helena Lopes, Maria A. |
dc.subject.por.fl_str_mv |
Ethylenediamine Hybrid medical device (PET/PLA) NH3 plasma Surface functionalization Tissue repair hybrid medical device (PET PLA) NH plasma 3 Science & Technology |
topic |
Ethylenediamine Hybrid medical device (PET/PLA) NH3 plasma Surface functionalization Tissue repair hybrid medical device (PET PLA) NH plasma 3 Science & Technology |
description |
Several polymers are researched for tendon repair as polyethylene terephthalate (PET) and polylactic acid (PLA). These are biocompatible and useful in scaffolding repair though with minimal success due to long-term failure. There is a need to improve such scaffolds’ design and physical–chemical nature. This work concerns surface functionalization of polymeric braids (PET and PLA) that fulfill the high mechanical demands of tissues such as tendons. The functionalization aims to incorporate amine groups in the braids’ surface, improve cell adhesion, and consequently, the poor healing rate of these tissues and the biointegration of the braids. Two approaches are compared: the direct application of NH3 plasma and the surface grafting of EDA after O2 plasma activation. X-ray photoelectron spectroscopy (XPS) shows that amine groups are effectively introduced onto the samples’ surfaces. Besides, the plasma parameters chosen do not compromise the topography and tensile behavior of the braids. Resazurin assay and scanning electron microscopy show that the NH3 treatment improves cell–biomaterial interaction as improved cell adhesion and proliferation are observed. Both approaches are safe for biomedical applications. The NH3 plasma approach is more environmentally friendly, faster, and easier to scale-up, showing potential for application in the final hybrid medical device. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/87912 |
url |
https://hdl.handle.net/1822/87912 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Peixoto, T., Silva, D., Rodrigues, M., Neto, M., Silva, R., Paiva, M. C., … Lopes, M. A. (2022, October 26). Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison. Macromolecular Materials and Engineering. Wiley. http://doi.org/10.1002/mame.202200426 1438-7492 10.1002/mame.202200426 https://onlinelibrary.wiley.com/doi/10.1002/mame.202200426 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136784803692544 |