Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11319 |
Resumo: | Here we present the results of a multiple organizational level analysis conceived to identify acclimative/adaptive strategies exhibited by the seagrass Posidonia oceanica to the daily fluctuations in the light environment, at contrasting depths. We assessed changes in photophysiological parameters, leaf respiration, pigments, and protein and mRNA expression levels. The results show that the diel oscillations of P. oceanica photophysiological and respiratory responses were related to transcripts and proteins expression of the genes involved in those processes and that there was a response asynchrony between shallow and deep plants probably caused by the strong differences in the light environment. The photochemical pathway of energy use was more effective in shallow plants due to higher light availability, but these plants needed more investment in photoprotection and photorepair, requiring higher translation and protein synthesis than deep plants. The genetic differentiation between deep and shallow stands suggests the existence of locally adapted genotypes to contrasting light environments. The depth-specific diel rhythms of photosynthetic and respiratory processes, from molecular to physiological levels, must be considered in the management and conservation of these key coastal ecosystems. |
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Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanicaEvolving enhancer protein-2Circadian-rhythmsPhotosystem-IiThalassia-TestudinumIn-SituPhotosynthetic characteristicsChlorophyll fluorescenceHalophila-StipulaceaArabidopsis-ThalianaConstant conditionsHere we present the results of a multiple organizational level analysis conceived to identify acclimative/adaptive strategies exhibited by the seagrass Posidonia oceanica to the daily fluctuations in the light environment, at contrasting depths. We assessed changes in photophysiological parameters, leaf respiration, pigments, and protein and mRNA expression levels. The results show that the diel oscillations of P. oceanica photophysiological and respiratory responses were related to transcripts and proteins expression of the genes involved in those processes and that there was a response asynchrony between shallow and deep plants probably caused by the strong differences in the light environment. The photochemical pathway of energy use was more effective in shallow plants due to higher light availability, but these plants needed more investment in photoprotection and photorepair, requiring higher translation and protein synthesis than deep plants. The genetic differentiation between deep and shallow stands suggests the existence of locally adapted genotypes to contrasting light environments. The depth-specific diel rhythms of photosynthetic and respiratory processes, from molecular to physiological levels, must be considered in the management and conservation of these key coastal ecosystems.Portuguese funds from FCT - Foundation for Science and Technology [UID/Multi/04326/2013]; SZN PhD fellowship via the Open University; ESF COST Action Seagrass Productivity: From Genes to Ecosystem Management [ES0906]Nature Publishing GroupSapientiaProcaccini, GabrieleRuocco, MiriamMarin-Guirao, LazaroDattolo, EmanuelaBrunet, ChristopheD'Esposito, DanielaLauritano, ChiaraMazzuca, SilviaSerra, Ilia AnnaBernardo, LetiziaPiro, AmaliaBeer, SvenBjork, MatsGullström, MartinBuapet, PimchanokRasmusson, Lina M.Felisberto, PauloGobert, SylvieRuncie, John W.Silva, JoãoOlive, IreneCosta, Monya M.Barrote, IsabelSantos, Rui2018-12-07T14:53:02Z2017-022017-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11319eng2045-232210.1038/srep42890info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:06Zoai:sapientia.ualg.pt:10400.1/11319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:02:50.526418Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica |
title |
Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica |
spellingShingle |
Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica Procaccini, Gabriele Evolving enhancer protein-2 Circadian-rhythms Photosystem-Ii Thalassia-Testudinum In-Situ Photosynthetic characteristics Chlorophyll fluorescence Halophila-Stipulacea Arabidopsis-Thaliana Constant conditions |
title_short |
Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica |
title_full |
Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica |
title_fullStr |
Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica |
title_full_unstemmed |
Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica |
title_sort |
Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica |
author |
Procaccini, Gabriele |
author_facet |
Procaccini, Gabriele Ruocco, Miriam Marin-Guirao, Lazaro Dattolo, Emanuela Brunet, Christophe D'Esposito, Daniela Lauritano, Chiara Mazzuca, Silvia Serra, Ilia Anna Bernardo, Letizia Piro, Amalia Beer, Sven Bjork, Mats Gullström, Martin Buapet, Pimchanok Rasmusson, Lina M. Felisberto, Paulo Gobert, Sylvie Runcie, John W. Silva, João Olive, Irene Costa, Monya M. Barrote, Isabel Santos, Rui |
author_role |
author |
author2 |
Ruocco, Miriam Marin-Guirao, Lazaro Dattolo, Emanuela Brunet, Christophe D'Esposito, Daniela Lauritano, Chiara Mazzuca, Silvia Serra, Ilia Anna Bernardo, Letizia Piro, Amalia Beer, Sven Bjork, Mats Gullström, Martin Buapet, Pimchanok Rasmusson, Lina M. Felisberto, Paulo Gobert, Sylvie Runcie, John W. Silva, João Olive, Irene Costa, Monya M. Barrote, Isabel Santos, Rui |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Procaccini, Gabriele Ruocco, Miriam Marin-Guirao, Lazaro Dattolo, Emanuela Brunet, Christophe D'Esposito, Daniela Lauritano, Chiara Mazzuca, Silvia Serra, Ilia Anna Bernardo, Letizia Piro, Amalia Beer, Sven Bjork, Mats Gullström, Martin Buapet, Pimchanok Rasmusson, Lina M. Felisberto, Paulo Gobert, Sylvie Runcie, John W. Silva, João Olive, Irene Costa, Monya M. Barrote, Isabel Santos, Rui |
dc.subject.por.fl_str_mv |
Evolving enhancer protein-2 Circadian-rhythms Photosystem-Ii Thalassia-Testudinum In-Situ Photosynthetic characteristics Chlorophyll fluorescence Halophila-Stipulacea Arabidopsis-Thaliana Constant conditions |
topic |
Evolving enhancer protein-2 Circadian-rhythms Photosystem-Ii Thalassia-Testudinum In-Situ Photosynthetic characteristics Chlorophyll fluorescence Halophila-Stipulacea Arabidopsis-Thaliana Constant conditions |
description |
Here we present the results of a multiple organizational level analysis conceived to identify acclimative/adaptive strategies exhibited by the seagrass Posidonia oceanica to the daily fluctuations in the light environment, at contrasting depths. We assessed changes in photophysiological parameters, leaf respiration, pigments, and protein and mRNA expression levels. The results show that the diel oscillations of P. oceanica photophysiological and respiratory responses were related to transcripts and proteins expression of the genes involved in those processes and that there was a response asynchrony between shallow and deep plants probably caused by the strong differences in the light environment. The photochemical pathway of energy use was more effective in shallow plants due to higher light availability, but these plants needed more investment in photoprotection and photorepair, requiring higher translation and protein synthesis than deep plants. The genetic differentiation between deep and shallow stands suggests the existence of locally adapted genotypes to contrasting light environments. The depth-specific diel rhythms of photosynthetic and respiratory processes, from molecular to physiological levels, must be considered in the management and conservation of these key coastal ecosystems. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02 2017-02-01T00:00:00Z 2018-12-07T14:53:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11319 |
url |
http://hdl.handle.net/10400.1/11319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-2322 10.1038/srep42890 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133262528905216 |