Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis

Detalhes bibliográficos
Autor(a) principal: Godinho, Lia M.
Data de Publicação: 2011
Outros Autores: Sá-Nogueira, Isabel de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/6165
Resumo: FEBS journal, Volume 278, Issue 14, pages 2511-2524, July 2011
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spelling Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilisGene regulationAraLBacillus subtilisHAD superfamily (IIA)Sugar phosphataseFEBS journal, Volume 278, Issue 14, pages 2511-2524, July 2011AraL from Bacillus subtilis is a member of the ubiquitous haloalkanoate dehalogenase superfamily. The araL gene has been cloned, over-expressed in Escherichia coli and its product purified to homogeneity. The enzyme displays phosphatase activity, which is optimal at neutral pH (7.0) and 65 C. Substrate screening and kinetic analysis showed AraL to have low specificity and catalytic activity towards several sugar phosphates, which are metabolic intermediates of the glycolytic and pentose phosphate pathways. On the basis of substrate specificity and gene context within the arabinose metabolic operon, a putative physiological role of AraL in the detoxification of accidental accumulation of phosphorylated metabolites has been proposed. The ability of AraL to catabolize several related secondary metabolites requires regulation at the genetic level. In the present study, using site-directed mutagenesis, we show that the production of AraL is regulated by a structure in the translation initiation region of the mRNA, which most probably blocks access to the ribosome-binding site, preventing protein synthesis. Members of haloalkanoate dehalogenase subfamily IIA and IIB are characterized by a broad-range and overlapping specificity anticipating the need for regulation at the genetic level. We provide evidence for the existence of a genetic regulatory mechanism controlling the production of AraL.This work was partially funded by grant no. PPCDT⁄BIA-MIC⁄ 61140 ⁄ 2004 from Fundacao para a Ciencia e Tecnologia, POCI and FEDER to I.S.-N.Wiley-BlackwellRUNGodinho, Lia M.Sá-Nogueira, Isabel de2011-10-21T15:29:27Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/6165engFEBS Journal 278 (2011) 2511–25241742-465810.1111/j.1742-4658.2011.08177.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:37:13Zoai:run.unl.pt:10362/6165Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:16:45.137720Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
title Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
spellingShingle Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
Godinho, Lia M.
Gene regulation
AraL
Bacillus subtilis
HAD superfamily (IIA)
Sugar phosphatase
title_short Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
title_full Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
title_fullStr Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
title_full_unstemmed Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
title_sort Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
author Godinho, Lia M.
author_facet Godinho, Lia M.
Sá-Nogueira, Isabel de
author_role author
author2 Sá-Nogueira, Isabel de
author2_role author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Godinho, Lia M.
Sá-Nogueira, Isabel de
dc.subject.por.fl_str_mv Gene regulation
AraL
Bacillus subtilis
HAD superfamily (IIA)
Sugar phosphatase
topic Gene regulation
AraL
Bacillus subtilis
HAD superfamily (IIA)
Sugar phosphatase
description FEBS journal, Volume 278, Issue 14, pages 2511-2524, July 2011
publishDate 2011
dc.date.none.fl_str_mv 2011-10-21T15:29:27Z
2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/6165
url http://hdl.handle.net/10362/6165
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv FEBS Journal 278 (2011) 2511–2524
1742-4658
10.1111/j.1742-4658.2011.08177.x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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