Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres

Detalhes bibliográficos
Autor(a) principal: Cotas, João
Data de Publicação: 2020
Outros Autores: Marques, Vanda, Afonso, Marta B., Rodrigues, Cecília M. P., Pereira, Leonel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/106352
https://doi.org/10.3390/md18010050
Resumo: Gigartina pistillata is a red seaweed common in Figueira da Foz, Portugal. Here, the antitumour potential of G. pistillata carrageenan, with a known variable of the life cycle, the female gametophyte (FG) and tetrasporophyte (T) was evaluated against colorectal cancer stem cell (CSC) -enriched tumourspheres. FTIR-ATR analysis of G. pistillata carrageenan extracts indicated differences between life cycle phases, being FG a κ/ι hybrid carrageenan and T a ʎ/ξ hybrid. Both carrageenan extracts presented IC50 values inferior to 1 μg/mL in HT29-derived CSC-enriched tumourspheres, as well as reduced tumoursphere area. The two extracts were also effective at reducing cellular viability in SW620- and SW480-derived tumourspheres. These results indicate that carrageenans extracted from two G. pistillata life cycle phases have antitumour potential against colorectal cancer stem-like cells, specially the T carrageenan.
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spelling Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheresantitumour activitycarrageenancolorectal cancercancer stem cellsAntineoplastic AgentsBiological ProductsCarrageenanCell Line, TumorCell SurvivalColorectal NeoplasmsHumansInhibitory Concentration 50Neoplastic Stem CellsRhodophytaSeaweedGigartina pistillata is a red seaweed common in Figueira da Foz, Portugal. Here, the antitumour potential of G. pistillata carrageenan, with a known variable of the life cycle, the female gametophyte (FG) and tetrasporophyte (T) was evaluated against colorectal cancer stem cell (CSC) -enriched tumourspheres. FTIR-ATR analysis of G. pistillata carrageenan extracts indicated differences between life cycle phases, being FG a κ/ι hybrid carrageenan and T a ʎ/ξ hybrid. Both carrageenan extracts presented IC50 values inferior to 1 μg/mL in HT29-derived CSC-enriched tumourspheres, as well as reduced tumoursphere area. The two extracts were also effective at reducing cellular viability in SW620- and SW480-derived tumourspheres. These results indicate that carrageenans extracted from two G. pistillata life cycle phases have antitumour potential against colorectal cancer stem-like cells, specially the T carrageenan.MDPI2020-01-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/106352http://hdl.handle.net/10316/106352https://doi.org/10.3390/md18010050eng1660-3397Cotas, JoãoMarques, VandaAfonso, Marta B.Rodrigues, Cecília M. P.Pereira, Leonelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-31T20:35:59Zoai:estudogeral.uc.pt:10316/106352Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:22:49.313994Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
title Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
spellingShingle Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
Cotas, João
antitumour activity
carrageenan
colorectal cancer
cancer stem cells
Antineoplastic Agents
Biological Products
Carrageenan
Cell Line, Tumor
Cell Survival
Colorectal Neoplasms
Humans
Inhibitory Concentration 50
Neoplastic Stem Cells
Rhodophyta
Seaweed
title_short Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
title_full Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
title_fullStr Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
title_full_unstemmed Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
title_sort Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
author Cotas, João
author_facet Cotas, João
Marques, Vanda
Afonso, Marta B.
Rodrigues, Cecília M. P.
Pereira, Leonel
author_role author
author2 Marques, Vanda
Afonso, Marta B.
Rodrigues, Cecília M. P.
Pereira, Leonel
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cotas, João
Marques, Vanda
Afonso, Marta B.
Rodrigues, Cecília M. P.
Pereira, Leonel
dc.subject.por.fl_str_mv antitumour activity
carrageenan
colorectal cancer
cancer stem cells
Antineoplastic Agents
Biological Products
Carrageenan
Cell Line, Tumor
Cell Survival
Colorectal Neoplasms
Humans
Inhibitory Concentration 50
Neoplastic Stem Cells
Rhodophyta
Seaweed
topic antitumour activity
carrageenan
colorectal cancer
cancer stem cells
Antineoplastic Agents
Biological Products
Carrageenan
Cell Line, Tumor
Cell Survival
Colorectal Neoplasms
Humans
Inhibitory Concentration 50
Neoplastic Stem Cells
Rhodophyta
Seaweed
description Gigartina pistillata is a red seaweed common in Figueira da Foz, Portugal. Here, the antitumour potential of G. pistillata carrageenan, with a known variable of the life cycle, the female gametophyte (FG) and tetrasporophyte (T) was evaluated against colorectal cancer stem cell (CSC) -enriched tumourspheres. FTIR-ATR analysis of G. pistillata carrageenan extracts indicated differences between life cycle phases, being FG a κ/ι hybrid carrageenan and T a ʎ/ξ hybrid. Both carrageenan extracts presented IC50 values inferior to 1 μg/mL in HT29-derived CSC-enriched tumourspheres, as well as reduced tumoursphere area. The two extracts were also effective at reducing cellular viability in SW620- and SW480-derived tumourspheres. These results indicate that carrageenans extracted from two G. pistillata life cycle phases have antitumour potential against colorectal cancer stem-like cells, specially the T carrageenan.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-12
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/106352
http://hdl.handle.net/10316/106352
https://doi.org/10.3390/md18010050
url http://hdl.handle.net/10316/106352
https://doi.org/10.3390/md18010050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1660-3397
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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