Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation

Detalhes bibliográficos
Autor(a) principal: Moura, Carla
Data de Publicação: 2020
Outros Autores: Silva, João Carlos, Faria, Sofia, Fernandes, Paulo Rui, Silva, Cláudia Lobato da, Cabral, Joaquim Manuel Sampaio, Linhardt, Robert, Bártolo, Paulo Jorge, Ferreira, Frederico Castelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/8414
Resumo: This work was supported by FCT - Portuguese Foundation for Science and Technology through the projects UID/BIO/04565/2020, UID/Multi/04044/2019, UIDB/500022/2020, PTDC/BBB-BMC/5655/ 2014, and PRECISE e Accelerating progress toward the new era of precision medicine (PAC-PRECISE-LISBOA-01-0145- FEDER e 016394) and Stimuli2BioScaffold (FCT grant PTDC/EMESIS/ 32554/2017). Funding received from Programa Operacional Regional de Lisboa 2020 (Project No. 007317), MIT Portugal Program and through project POCI 01-0145-FEDER-016800 is also acknowledged. Carla Sofia Moura and João Carlos Silva are also thankful to FCT for funding received through the scholarships SFRH/BD/73970/2010 and SFRH/BD/105771/2014, respectively.
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spelling Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementationArticular cartilage tissue engineeringBone marrow mesenchymal stem/stromal cellsChondroitin sulfateFused deposition modelingHypoxiaPoly (ε-caprolactone) scaffoldsThis work was supported by FCT - Portuguese Foundation for Science and Technology through the projects UID/BIO/04565/2020, UID/Multi/04044/2019, UIDB/500022/2020, PTDC/BBB-BMC/5655/ 2014, and PRECISE e Accelerating progress toward the new era of precision medicine (PAC-PRECISE-LISBOA-01-0145- FEDER e 016394) and Stimuli2BioScaffold (FCT grant PTDC/EMESIS/ 32554/2017). Funding received from Programa Operacional Regional de Lisboa 2020 (Project No. 007317), MIT Portugal Program and through project POCI 01-0145-FEDER-016800 is also acknowledged. Carla Sofia Moura and João Carlos Silva are also thankful to FCT for funding received through the scholarships SFRH/BD/73970/2010 and SFRH/BD/105771/2014, respectively.Cartilage defects resultant from trauma or degenerative diseases (e.g., osteoarthritis) can potentially be repaired using tissue engineering (TE) strategies combining progenitor cells, biomaterial scaffolds and bio physical/chemical cues. This work examines promoting chondrogenic differentiation of human bone marrow mesenchymal stem/stromal cells (BMMSCs) by combining the effects of modified poly (ε-caprolactone) (PCL) scaffolds hydrophilicity and chondroitin sulfate (CS) supplementation in a hypoxic 5% oxygen atmosphere. 3D extruded PCL scaffolds, characterized by mCT, featured a 21 mmL1 surface area to volume ratio, 390 mm pore size and approximately 100% pore interconnectivity. Scaffold immersion in sodium hydroxide solutions for different periods of time had major effects in scaffold surface morphology, wettability and mechanical properties, but without improvements on cell adhesion. In-situ chondrogenic differentiation of BM-MSC seeded in 3D-extruded PCL scaffolds resulted in higher cell populations and ECM deposition along all scaffold structure, when chondrogenesis was preceded by an expansion phase. Additionally, CS supplementation during BM-MSC expansion was crucial to enhance aggrecan gene expression, known as a hallmark of chondrogenesis. Overall, this study presents an approach to tailor the wettability and mechanical properties of PCL scaffolds and supports the use of CSsupplementation as a biochemical cue in integrated TE strategies for cartilage regeneration.ElsevierIC-OnlineMoura, CarlaSilva, João CarlosFaria, SofiaFernandes, Paulo RuiSilva, Cláudia Lobato daCabral, Joaquim Manuel SampaioLinhardt, RobertBártolo, Paulo JorgeFerreira, Frederico Castelo2023-04-19T10:55:26Z2020-062020-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8414engMoura, C. S., Silva, J. C., Faria, S., Fernandes, P. R., da Silva, C. L., Cabral, J. M. S., Linhardt, R., Bártolo, P. J., & Ferreira, F. C. (2020). Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation. Journal of Bioscience and Bioengineering, 129(6), 756–764. https://doi.org/10.1016/j.jbiosc.2020.01.0041389-172310.1016/j.jbiosc.2020.01.004metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:57:16Zoai:iconline.ipleiria.pt:10400.8/8414Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:51:08.520657Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation
title Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation
spellingShingle Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation
Moura, Carla
Articular cartilage tissue engineering
Bone marrow mesenchymal stem/stromal cells
Chondroitin sulfate
Fused deposition modeling
Hypoxia
Poly (ε-caprolactone) scaffolds
title_short Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation
title_full Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation
title_fullStr Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation
title_full_unstemmed Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation
title_sort Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation
author Moura, Carla
author_facet Moura, Carla
Silva, João Carlos
Faria, Sofia
Fernandes, Paulo Rui
Silva, Cláudia Lobato da
Cabral, Joaquim Manuel Sampaio
Linhardt, Robert
Bártolo, Paulo Jorge
Ferreira, Frederico Castelo
author_role author
author2 Silva, João Carlos
Faria, Sofia
Fernandes, Paulo Rui
Silva, Cláudia Lobato da
Cabral, Joaquim Manuel Sampaio
Linhardt, Robert
Bártolo, Paulo Jorge
Ferreira, Frederico Castelo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Moura, Carla
Silva, João Carlos
Faria, Sofia
Fernandes, Paulo Rui
Silva, Cláudia Lobato da
Cabral, Joaquim Manuel Sampaio
Linhardt, Robert
Bártolo, Paulo Jorge
Ferreira, Frederico Castelo
dc.subject.por.fl_str_mv Articular cartilage tissue engineering
Bone marrow mesenchymal stem/stromal cells
Chondroitin sulfate
Fused deposition modeling
Hypoxia
Poly (ε-caprolactone) scaffolds
topic Articular cartilage tissue engineering
Bone marrow mesenchymal stem/stromal cells
Chondroitin sulfate
Fused deposition modeling
Hypoxia
Poly (ε-caprolactone) scaffolds
description This work was supported by FCT - Portuguese Foundation for Science and Technology through the projects UID/BIO/04565/2020, UID/Multi/04044/2019, UIDB/500022/2020, PTDC/BBB-BMC/5655/ 2014, and PRECISE e Accelerating progress toward the new era of precision medicine (PAC-PRECISE-LISBOA-01-0145- FEDER e 016394) and Stimuli2BioScaffold (FCT grant PTDC/EMESIS/ 32554/2017). Funding received from Programa Operacional Regional de Lisboa 2020 (Project No. 007317), MIT Portugal Program and through project POCI 01-0145-FEDER-016800 is also acknowledged. Carla Sofia Moura and João Carlos Silva are also thankful to FCT for funding received through the scholarships SFRH/BD/73970/2010 and SFRH/BD/105771/2014, respectively.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
2020-06-01T00:00:00Z
2023-04-19T10:55:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/8414
url http://hdl.handle.net/10400.8/8414
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Moura, C. S., Silva, J. C., Faria, S., Fernandes, P. R., da Silva, C. L., Cabral, J. M. S., Linhardt, R., Bártolo, P. J., & Ferreira, F. C. (2020). Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation. Journal of Bioscience and Bioengineering, 129(6), 756–764. https://doi.org/10.1016/j.jbiosc.2020.01.004
1389-1723
10.1016/j.jbiosc.2020.01.004
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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