Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/6370 |
Resumo: | The genus Mesorhizobium includes species nodulating several legumes, such as chickpea, which has a high agronomic importance. Chickpea rhizobia were originally described as either Mesorhizobium ciceri or M. mediterraneum. However, rhizobia able to nodulate chickpea have been shown to belong to several different species within the genus Mesorhizobium. The present study used a multilocus sequence analysis approach to infer a high resolution phylogeny of the genus Mesorhizobium and to confirm the existence of a new chickpea nodulating genospecies. The phylogenetic structure of the Mesorhizobium clade was evaluated by sequence analysis of the 16S rRNA gene, ITS region and the five core genes atpD, dnaJ, glnA, gyrB, and recA. Phylogenies obtained with the different genes are in overall good agreement and a well-supported, almost fully resolved, phylogenetic tree was obtained using the combined data. Our phylogenetic analyses of core genes sequences and their comparison with the symbiosis gene nodC, corroborate the existence of one new chickpea Mesorhizobium genospecies and one new symbiovar, M. opportunistum sv. ciceri. Furthermore, our results show that symbiovar ciceri spreads over six species of mesorhizobia. To our knowledge this study shows the most complete Mesorhizobium multilocus phylogeny to date and contributes to the understanding of how a symbiovar may be present in different species. (c) 2012 Elsevier GmbH. All rights reserved. |
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Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium speciesMesorhizobiumPhylogenyMultilocus sequence analysisSymbiovardnaJgyrBThe genus Mesorhizobium includes species nodulating several legumes, such as chickpea, which has a high agronomic importance. Chickpea rhizobia were originally described as either Mesorhizobium ciceri or M. mediterraneum. However, rhizobia able to nodulate chickpea have been shown to belong to several different species within the genus Mesorhizobium. The present study used a multilocus sequence analysis approach to infer a high resolution phylogeny of the genus Mesorhizobium and to confirm the existence of a new chickpea nodulating genospecies. The phylogenetic structure of the Mesorhizobium clade was evaluated by sequence analysis of the 16S rRNA gene, ITS region and the five core genes atpD, dnaJ, glnA, gyrB, and recA. Phylogenies obtained with the different genes are in overall good agreement and a well-supported, almost fully resolved, phylogenetic tree was obtained using the combined data. Our phylogenetic analyses of core genes sequences and their comparison with the symbiosis gene nodC, corroborate the existence of one new chickpea Mesorhizobium genospecies and one new symbiovar, M. opportunistum sv. ciceri. Furthermore, our results show that symbiovar ciceri spreads over six species of mesorhizobia. To our knowledge this study shows the most complete Mesorhizobium multilocus phylogeny to date and contributes to the understanding of how a symbiovar may be present in different species. (c) 2012 Elsevier GmbH. All rights reserved.2012-12-05T00:03:04Z2012-12-052012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/6370http://hdl.handle.net/10174/6370engLaranjo, Marta; Young, J. Peter W.; Oliveira, Solange. Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species, Systematic and Applied Microbiology, 35, 6, 359-367, 2012.0723-2020http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=1&SID=X2DDmHEGNcf5OpOMk@l&page=1&doc=1Publicações -artigos em revistas internacionais com arbitragem cientifica ICAAM e Departamento de Biologiamlaranjo@uevora.ptndismo@uevora.pt371Laranjo, MartaYoung, J. Peter W.Oliveira, Solangeinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:45:26Zoai:dspace.uevora.pt:10174/6370Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:01:00.855224Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species |
title |
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species |
spellingShingle |
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species Laranjo, Marta Mesorhizobium Phylogeny Multilocus sequence analysis Symbiovar dnaJ gyrB |
title_short |
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species |
title_full |
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species |
title_fullStr |
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species |
title_full_unstemmed |
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species |
title_sort |
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species |
author |
Laranjo, Marta |
author_facet |
Laranjo, Marta Young, J. Peter W. Oliveira, Solange |
author_role |
author |
author2 |
Young, J. Peter W. Oliveira, Solange |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Laranjo, Marta Young, J. Peter W. Oliveira, Solange |
dc.subject.por.fl_str_mv |
Mesorhizobium Phylogeny Multilocus sequence analysis Symbiovar dnaJ gyrB |
topic |
Mesorhizobium Phylogeny Multilocus sequence analysis Symbiovar dnaJ gyrB |
description |
The genus Mesorhizobium includes species nodulating several legumes, such as chickpea, which has a high agronomic importance. Chickpea rhizobia were originally described as either Mesorhizobium ciceri or M. mediterraneum. However, rhizobia able to nodulate chickpea have been shown to belong to several different species within the genus Mesorhizobium. The present study used a multilocus sequence analysis approach to infer a high resolution phylogeny of the genus Mesorhizobium and to confirm the existence of a new chickpea nodulating genospecies. The phylogenetic structure of the Mesorhizobium clade was evaluated by sequence analysis of the 16S rRNA gene, ITS region and the five core genes atpD, dnaJ, glnA, gyrB, and recA. Phylogenies obtained with the different genes are in overall good agreement and a well-supported, almost fully resolved, phylogenetic tree was obtained using the combined data. Our phylogenetic analyses of core genes sequences and their comparison with the symbiosis gene nodC, corroborate the existence of one new chickpea Mesorhizobium genospecies and one new symbiovar, M. opportunistum sv. ciceri. Furthermore, our results show that symbiovar ciceri spreads over six species of mesorhizobia. To our knowledge this study shows the most complete Mesorhizobium multilocus phylogeny to date and contributes to the understanding of how a symbiovar may be present in different species. (c) 2012 Elsevier GmbH. All rights reserved. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-05T00:03:04Z 2012-12-05 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/6370 http://hdl.handle.net/10174/6370 |
url |
http://hdl.handle.net/10174/6370 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Laranjo, Marta; Young, J. Peter W.; Oliveira, Solange. Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium species, Systematic and Applied Microbiology, 35, 6, 359-367, 2012. 0723-2020 http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=1&SID=X2DDmHEGNcf5OpOMk@l&page=1&doc=1 Publicações -artigos em revistas internacionais com arbitragem cientifica ICAAM e Departamento de Biologia mlaranjo@uevora.pt nd ismo@uevora.pt 371 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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