In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts

Detalhes bibliográficos
Autor(a) principal: Ferreira, Joana
Data de Publicação: 2020
Outros Autores: Santos, Sara, Pereira, Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/20341
Resumo: Research Article
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spelling In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extractscorkcorkback extractsantioxidant activityacetylcholinesteraseResearch ArticlePurpose. Acetylcholinesterase (AChE) inhibitors are used to treat Alzheimer’s patients because they enhance cholinergic neurotransmission. It is urgent to find new and efficient inhibitors from natural sources, highly bioavailable with low or no toxicity. +e plant kingdom is extremely rich in a variety of compounds that are potent AChE inhibitors: flavonoids and other phenolic compounds have been recognized as promising Alzheimer’s treatment agents. In this study, in vitro acetylcholinesterase inhibition, antioxidant activities, and total flavonoid and phenolic contents of ethanol-water extracts from Quercus suber cork and corkback were evaluated. Methods. +e acetylcholinesterase activity was determined by a colorimetric assay based on Ellman’s methodology. +e Folin–Ciocalteu colorimetric method was used for total phenolic content determination and the aluminium chloride method for the determination of total flavonoid content. Antioxidant activity assays were performed using the DPPH and FRAP assays. Results. +e acetylcholinesterase inhibitory activity from Q. suber cork and corkback ethanol-water extracts was as follows: 62% inhibition with corkback extracts over 0.5 mg/mL and around 49% inhibition in cork extracts over 1.0 mg/mL extracts’ concentration. Regarding the DPPH radical scavenging activity, the concentrations of cork and corkback ethanol-water extracts required for 50% DPPH inhibition (IC50) were 3.2 μg/mL and 4.0–5.2 μg/mL. Corkback extracts are less effective than Trolox standard (3.2 μg/mL) but cork extracts showed the same free radical scavenging activity compared to Trolox. Cork and corkback extracts have antioxidant power of 750.9–775.4 mg TEAC/g extract and 1051.2–2052.4 mg TEAC/g extract, respectively, which are significantly higher than the ones obtained with Trolox: 19.6–21.0 mg TEAC/g extract (cork assays) and 57.4–66.3 mg TEAC/g extract (corkback assays). +e amounts of total phenolic (TPC) and flavonoid (TFC) compounds were 8.7–32.3 mg GAE/ g and 4.8–10.7 mg CE/g dry mass for cork and 5.4–5.7 mg GAE/g and 42.5 mg CE/g dry mass for corkback extracts, respectively, using catechin (CE) and GAE (gallic acid) as standards. Conclusion. +ese findings demonstrate the remarkable potential of these extracts as valuable source of antioxidants with interesting acetylcholinesterase inhibitory activityHindawiRepositório da Universidade de LisboaFerreira, JoanaSantos, SaraPereira, Helena2020-09-17T13:54:08Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/20341engEvidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 3825629, 8 pageshttps://doi.org/10.1155/2020/3825629info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:49:46Zoai:www.repository.utl.pt:10400.5/20341Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:05:06.635353Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
title In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
spellingShingle In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
Ferreira, Joana
cork
corkback extracts
antioxidant activity
acetylcholinesterase
title_short In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
title_full In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
title_fullStr In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
title_full_unstemmed In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
title_sort In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
author Ferreira, Joana
author_facet Ferreira, Joana
Santos, Sara
Pereira, Helena
author_role author
author2 Santos, Sara
Pereira, Helena
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Ferreira, Joana
Santos, Sara
Pereira, Helena
dc.subject.por.fl_str_mv cork
corkback extracts
antioxidant activity
acetylcholinesterase
topic cork
corkback extracts
antioxidant activity
acetylcholinesterase
description Research Article
publishDate 2020
dc.date.none.fl_str_mv 2020-09-17T13:54:08Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/20341
url http://hdl.handle.net/10400.5/20341
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 3825629, 8 pages
https://doi.org/10.1155/2020/3825629
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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