Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31998 |
Resumo: | High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clostridium pasteurianum DSM 525 is studied. Also, the effect of acetate and butyrate supplementation to the culture medium and the culture medium composition are evaluated. The results showed a marked effect of crude glycerol concentration on the product yield. The competitive nature of butanol and 1,3-PDO pathways has been evident, and a shift to the butanol pathway once using higher substrate concentrations (up to 35 g l 1) was clearly observed. Butyrate supplementation to the culture medium resulted in a 45% higher butanol titre, a lower production of 1,3-PDO and it decreased the fermentation time. Acetate supplementation also increased the butanol titre but the fermentation was longer. Even though glycerol consumption could not be increased over 32 g l 1, when the concentrations of NH4Cl and FeCl2 were simultaneously increased, the results obtained were similar to those observed when butyrate was supplemented to the culture medium; a 35% higher butanol yield at the expense of 1,3-PDO and a shorter fermentation. The results herein gathered suggest that there are other factors besides butanol inhibition and nutrient limitation that affect the glycerol consumption. |
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Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanolButanolClostridium pasteurianumCrude glycerolButyrate1,3-PropanediolScience & TechnologyHigh production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clostridium pasteurianum DSM 525 is studied. Also, the effect of acetate and butyrate supplementation to the culture medium and the culture medium composition are evaluated. The results showed a marked effect of crude glycerol concentration on the product yield. The competitive nature of butanol and 1,3-PDO pathways has been evident, and a shift to the butanol pathway once using higher substrate concentrations (up to 35 g l 1) was clearly observed. Butyrate supplementation to the culture medium resulted in a 45% higher butanol titre, a lower production of 1,3-PDO and it decreased the fermentation time. Acetate supplementation also increased the butanol titre but the fermentation was longer. Even though glycerol consumption could not be increased over 32 g l 1, when the concentrations of NH4Cl and FeCl2 were simultaneously increased, the results obtained were similar to those observed when butyrate was supplemented to the culture medium; a 35% higher butanol yield at the expense of 1,3-PDO and a shorter fermentation. The results herein gathered suggest that there are other factors besides butanol inhibition and nutrient limitation that affect the glycerol consumption.The authors acknowledge the financial support from the Strategic Project PEst-OE/EQB/LA0023/2013; the project ref. RECI/BBB-EBI/0179/2012 (project number FCOMP-01-0124-FEDER-027462); and the PhD grant given to R. Gallardo (ref SFRH/BD/42900/2008) funded by Fundacao para a Ciencia e a Tecnologia. The authors thank the MIT-Portugal Program for the support given to R. Gallardo.Pergamon Press Ltd.Universidade do MinhoGallardo, R.Alves, M. M.Rodrigues, L. R.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31998eng0961-953410.1016/j.biombioe.2014.10.015info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:44Zoai:repositorium.sdum.uminho.pt:1822/31998Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:11.861024Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol |
title |
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol |
spellingShingle |
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol Gallardo, R. Butanol Clostridium pasteurianum Crude glycerol Butyrate 1,3-Propanediol Science & Technology |
title_short |
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol |
title_full |
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol |
title_fullStr |
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol |
title_full_unstemmed |
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol |
title_sort |
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol |
author |
Gallardo, R. |
author_facet |
Gallardo, R. Alves, M. M. Rodrigues, L. R. |
author_role |
author |
author2 |
Alves, M. M. Rodrigues, L. R. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gallardo, R. Alves, M. M. Rodrigues, L. R. |
dc.subject.por.fl_str_mv |
Butanol Clostridium pasteurianum Crude glycerol Butyrate 1,3-Propanediol Science & Technology |
topic |
Butanol Clostridium pasteurianum Crude glycerol Butyrate 1,3-Propanediol Science & Technology |
description |
High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clostridium pasteurianum DSM 525 is studied. Also, the effect of acetate and butyrate supplementation to the culture medium and the culture medium composition are evaluated. The results showed a marked effect of crude glycerol concentration on the product yield. The competitive nature of butanol and 1,3-PDO pathways has been evident, and a shift to the butanol pathway once using higher substrate concentrations (up to 35 g l 1) was clearly observed. Butyrate supplementation to the culture medium resulted in a 45% higher butanol titre, a lower production of 1,3-PDO and it decreased the fermentation time. Acetate supplementation also increased the butanol titre but the fermentation was longer. Even though glycerol consumption could not be increased over 32 g l 1, when the concentrations of NH4Cl and FeCl2 were simultaneously increased, the results obtained were similar to those observed when butyrate was supplemented to the culture medium; a 35% higher butanol yield at the expense of 1,3-PDO and a shorter fermentation. The results herein gathered suggest that there are other factors besides butanol inhibition and nutrient limitation that affect the glycerol consumption. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31998 |
url |
http://hdl.handle.net/1822/31998 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0961-9534 10.1016/j.biombioe.2014.10.015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon Press Ltd. |
publisher.none.fl_str_mv |
Pergamon Press Ltd. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133127005700096 |