Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/18566 |
Resumo: | Here is presented a highly sensitive biomimetic sensor for the detection of an Alzheimer’s Disease (AD) biomarker, interleukin-6 (IL6), for point-of-care (PoC) analysis. The imprinted polymeric film was prepared by the co-electropolymerization of pyrrole (Py) and carboxylated pyrrole in the presence of IL6, on a carbon-screen printed electrode . The biomarker molecule was then removed by oxalic acid , creating the recognition vacant sites. Similarly, a control was also prepared in absense of the IL6 biomarker . The different steps of the sensor fabrication were characterized by Raman Spectroscopy, Electrochemical Impedance Spectroscopy (EIS), and Cyclic Voltammetry. Biomarker recognition and capture capacity of the sensing material was measured by EIS. The biomimetic sensor showed a limit of detection for IL6 of 0.02 pg/mL in spiked serum samples. Overall, the biosensing device showed good sensitivity, reproducibility, accuracy, and rapid response time, which contibute for the development of early diagnostics Poc tools for neurological diseases. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE)Carbon-screen printed electrode (C-SPE)Cyclic voltammetry (CV)Electrochemical impedance spectroscopy (EIS)Interleukin-6 (IL-6)Point-of care (PoC) biosensorpyrrole (Py) molecularly imprinted polymer (MIP)Here is presented a highly sensitive biomimetic sensor for the detection of an Alzheimer’s Disease (AD) biomarker, interleukin-6 (IL6), for point-of-care (PoC) analysis. The imprinted polymeric film was prepared by the co-electropolymerization of pyrrole (Py) and carboxylated pyrrole in the presence of IL6, on a carbon-screen printed electrode . The biomarker molecule was then removed by oxalic acid , creating the recognition vacant sites. Similarly, a control was also prepared in absense of the IL6 biomarker . The different steps of the sensor fabrication were characterized by Raman Spectroscopy, Electrochemical Impedance Spectroscopy (EIS), and Cyclic Voltammetry. Biomarker recognition and capture capacity of the sensing material was measured by EIS. The biomimetic sensor showed a limit of detection for IL6 of 0.02 pg/mL in spiked serum samples. Overall, the biosensing device showed good sensitivity, reproducibility, accuracy, and rapid response time, which contibute for the development of early diagnostics Poc tools for neurological diseases.POCTEP/INTERREG is acknowledged through the project 2QBioneuro, Impulso de una red de I + i en química biológica para diagnóstico y tratamiento de enfermedades neurológicas. ERA-Net, FCT/JPND cofunded Oligomer-Focused Screening and Individualized Therapeutics to target Neurodegenerative Disorders.Taylor and FrancisRepositório Científico do Instituto Politécnico do PortoGonçalves, M. de LurdesTruta, LilianaSales, Maria Goreti FerreiraMoreira, Felismina2022-02-01T01:30:26Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18566eng0003-271910.1080/00032719.2021.1879108info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:09:54Zoai:recipp.ipp.pt:10400.22/18566Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:37:54.922379Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE) |
title |
Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE) |
spellingShingle |
Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE) Gonçalves, M. de Lurdes Carbon-screen printed electrode (C-SPE) Cyclic voltammetry (CV) Electrochemical impedance spectroscopy (EIS) Interleukin-6 (IL-6) Point-of care (PoC) biosensor pyrrole (Py) molecularly imprinted polymer (MIP) |
title_short |
Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE) |
title_full |
Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE) |
title_fullStr |
Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE) |
title_full_unstemmed |
Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE) |
title_sort |
Electrochemical Point-of Care (PoC) Determination of Interleukin-6 (IL-6) Using a Pyrrole (Py) Molecularly Imprinted Polymer (MIP) on a Carbon-Screen Printed Electrode (C-SPE) |
author |
Gonçalves, M. de Lurdes |
author_facet |
Gonçalves, M. de Lurdes Truta, Liliana Sales, Maria Goreti Ferreira Moreira, Felismina |
author_role |
author |
author2 |
Truta, Liliana Sales, Maria Goreti Ferreira Moreira, Felismina |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Gonçalves, M. de Lurdes Truta, Liliana Sales, Maria Goreti Ferreira Moreira, Felismina |
dc.subject.por.fl_str_mv |
Carbon-screen printed electrode (C-SPE) Cyclic voltammetry (CV) Electrochemical impedance spectroscopy (EIS) Interleukin-6 (IL-6) Point-of care (PoC) biosensor pyrrole (Py) molecularly imprinted polymer (MIP) |
topic |
Carbon-screen printed electrode (C-SPE) Cyclic voltammetry (CV) Electrochemical impedance spectroscopy (EIS) Interleukin-6 (IL-6) Point-of care (PoC) biosensor pyrrole (Py) molecularly imprinted polymer (MIP) |
description |
Here is presented a highly sensitive biomimetic sensor for the detection of an Alzheimer’s Disease (AD) biomarker, interleukin-6 (IL6), for point-of-care (PoC) analysis. The imprinted polymeric film was prepared by the co-electropolymerization of pyrrole (Py) and carboxylated pyrrole in the presence of IL6, on a carbon-screen printed electrode . The biomarker molecule was then removed by oxalic acid , creating the recognition vacant sites. Similarly, a control was also prepared in absense of the IL6 biomarker . The different steps of the sensor fabrication were characterized by Raman Spectroscopy, Electrochemical Impedance Spectroscopy (EIS), and Cyclic Voltammetry. Biomarker recognition and capture capacity of the sensing material was measured by EIS. The biomimetic sensor showed a limit of detection for IL6 of 0.02 pg/mL in spiked serum samples. Overall, the biosensing device showed good sensitivity, reproducibility, accuracy, and rapid response time, which contibute for the development of early diagnostics Poc tools for neurological diseases. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-02-01T01:30:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/18566 |
url |
http://hdl.handle.net/10400.22/18566 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0003-2719 10.1080/00032719.2021.1879108 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor and Francis |
publisher.none.fl_str_mv |
Taylor and Francis |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131469035077632 |