Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers

Detalhes bibliográficos
Autor(a) principal: Lourenço, António
Data de Publicação: 2011
Outros Autores: Machado, Henrique, Brito, Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/4455
Resumo: Available at B-on
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spelling Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizersbiofilmsCalgary biofilm deviceListeria monocytogeneslow temperaturesanitizersAvailable at B-onThe biofilm-forming ability of 21 Listeria monocytogenes isolates, previously pulsotyped and corresponding to 16 strains, from different origins was evaluated using the Calgary Biofilm DeviceR , at 37 ◦C. Biofilms of 4 selected strains were also produced either on pure cultures or on co-cultures with Pseudomonas aeruginosa (PAO1), at 12 ◦C and at 37 ◦C. For these biofilms, the minimum biofilm eradication concentrations (MBECs) of 4 commercial dairy sanitizers (1 alkyl amine acetate based—T99, 2 chlorine based—T66 and DD, and 1 phosphoric acid based—BP) were determined. Listeria monocytogenes biofilms grown, either at 37 ◦C or 12 ◦C, were able to achieve similar cell densities by using different incubation periods (24 h and 7 d, respectively). In co-culture biofilms, P. aeruginosa was the dominant species, either at 37 ◦C or at 12 ◦C, representing 99% of a total biofilm population of 6 to 7 log CFU/peg. Co-culture biofilms were generally less susceptible than L. monocytogenes pure cultures. More interestingly, the biofilms produced at 12 ◦C were usually less susceptible to the sanitizers than when produced at 37 ◦C. Single or co-culture biofilms of L. monocytogenes and PAO1, particularly produced at 12 ◦C, retrieved MBEC values for agents T99 and BP that were, at times, above the maximum in-use recommended concentrations for these agents. The results presented here reinforce the importance of the temperature used for biofilm formation, when susceptibility to sanitizers is being assessedWiley-BlackwellRepositório da Universidade de LisboaLourenço, AntónioMachado, HenriqueBrito, Luísa2012-06-21T10:46:56Z2011-032011-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/4455eng"Journal of Food Science". ISSN 1750-3841. 76(2) (2011) M143-M1481750-3841info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:35:26Zoai:www.repository.utl.pt:10400.5/4455Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:52:06.591553Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
title Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
spellingShingle Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
Lourenço, António
biofilms
Calgary biofilm device
Listeria monocytogenes
low temperature
sanitizers
title_short Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
title_full Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
title_fullStr Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
title_full_unstemmed Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
title_sort Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
author Lourenço, António
author_facet Lourenço, António
Machado, Henrique
Brito, Luísa
author_role author
author2 Machado, Henrique
Brito, Luísa
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Lourenço, António
Machado, Henrique
Brito, Luísa
dc.subject.por.fl_str_mv biofilms
Calgary biofilm device
Listeria monocytogenes
low temperature
sanitizers
topic biofilms
Calgary biofilm device
Listeria monocytogenes
low temperature
sanitizers
description Available at B-on
publishDate 2011
dc.date.none.fl_str_mv 2011-03
2011-03-01T00:00:00Z
2012-06-21T10:46:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/4455
url http://hdl.handle.net/10400.5/4455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Food Science". ISSN 1750-3841. 76(2) (2011) M143-M148
1750-3841
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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