Separation microfluidic device fabricated by micromilling techniques
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/75818 |
Resumo: | The diagnosis of several diseases can be performed by analyzing the blood plasma of a patient. Despite extensive research work, there is still a need to improve current low-cost fabrication techniques and devices for the separation of plasma from blood cells. Microfluidic biomedical devices have great potential for that process. Hence, a microfluidic device made by micromilling and sealed with an oxygen plasma technique was tested by means of two different blood analogue fluids. The device has four microchannels with similar geometries but different channel depths. A high-speed video microscopy system was used for the visualization and acquisition of the flow of the analogue fluids throughout the microchannels of the device. Then, the separation of particles and plasma was evaluated with the ImageJ software by measuring and comparing the grey values at the entrance and the exit of the channel. The device showed a significant reduction of the amount of cells between the entrance and the exit of the microchannels. The depth of the channels and the size of the particles were not found to exert any major influence on the separation process. However, it was found that the flow rate affected the separation results, as the best results were obtained for a flow rate of 100 μL/min. Though these results are promising, further analyses and optimizations of microfluidic devices, as well as comparisons between devices sealed using different methods such as the solvent bonding technique, will be conducted in future works. |
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Separation microfluidic device fabricated by micromilling techniquesMicromillingMicrofluidic devicesParticle separationBlood analoguesImage analysisThe diagnosis of several diseases can be performed by analyzing the blood plasma of a patient. Despite extensive research work, there is still a need to improve current low-cost fabrication techniques and devices for the separation of plasma from blood cells. Microfluidic biomedical devices have great potential for that process. Hence, a microfluidic device made by micromilling and sealed with an oxygen plasma technique was tested by means of two different blood analogue fluids. The device has four microchannels with similar geometries but different channel depths. A high-speed video microscopy system was used for the visualization and acquisition of the flow of the analogue fluids throughout the microchannels of the device. Then, the separation of particles and plasma was evaluated with the ImageJ software by measuring and comparing the grey values at the entrance and the exit of the channel. The device showed a significant reduction of the amount of cells between the entrance and the exit of the microchannels. The depth of the channels and the size of the particles were not found to exert any major influence on the separation process. However, it was found that the flow rate affected the separation results, as the best results were obtained for a flow rate of 100 μL/min. Though these results are promising, further analyses and optimizations of microfluidic devices, as well as comparisons between devices sealed using different methods such as the solvent bonding technique, will be conducted in future works.MDPIUniversidade do MinhoGonçalves, Inês MaiaMadureira, MiguelMiranda, InêsSchütte, HelmutMoita, AnaMinas, GraçaGassmann, StefanLima, Rui Alberto Madeira Macedo2021-04-162021-04-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75818eng2673-459110.3390/Micromachines2021-09599https://www.mdpi.com/2673-4591/4/1/37info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:05:34Zoai:repositorium.sdum.uminho.pt:1822/75818Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:02.253402Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Separation microfluidic device fabricated by micromilling techniques |
title |
Separation microfluidic device fabricated by micromilling techniques |
spellingShingle |
Separation microfluidic device fabricated by micromilling techniques Gonçalves, Inês Maia Micromilling Microfluidic devices Particle separation Blood analogues Image analysis |
title_short |
Separation microfluidic device fabricated by micromilling techniques |
title_full |
Separation microfluidic device fabricated by micromilling techniques |
title_fullStr |
Separation microfluidic device fabricated by micromilling techniques |
title_full_unstemmed |
Separation microfluidic device fabricated by micromilling techniques |
title_sort |
Separation microfluidic device fabricated by micromilling techniques |
author |
Gonçalves, Inês Maia |
author_facet |
Gonçalves, Inês Maia Madureira, Miguel Miranda, Inês Schütte, Helmut Moita, Ana Minas, Graça Gassmann, Stefan Lima, Rui Alberto Madeira Macedo |
author_role |
author |
author2 |
Madureira, Miguel Miranda, Inês Schütte, Helmut Moita, Ana Minas, Graça Gassmann, Stefan Lima, Rui Alberto Madeira Macedo |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gonçalves, Inês Maia Madureira, Miguel Miranda, Inês Schütte, Helmut Moita, Ana Minas, Graça Gassmann, Stefan Lima, Rui Alberto Madeira Macedo |
dc.subject.por.fl_str_mv |
Micromilling Microfluidic devices Particle separation Blood analogues Image analysis |
topic |
Micromilling Microfluidic devices Particle separation Blood analogues Image analysis |
description |
The diagnosis of several diseases can be performed by analyzing the blood plasma of a patient. Despite extensive research work, there is still a need to improve current low-cost fabrication techniques and devices for the separation of plasma from blood cells. Microfluidic biomedical devices have great potential for that process. Hence, a microfluidic device made by micromilling and sealed with an oxygen plasma technique was tested by means of two different blood analogue fluids. The device has four microchannels with similar geometries but different channel depths. A high-speed video microscopy system was used for the visualization and acquisition of the flow of the analogue fluids throughout the microchannels of the device. Then, the separation of particles and plasma was evaluated with the ImageJ software by measuring and comparing the grey values at the entrance and the exit of the channel. The device showed a significant reduction of the amount of cells between the entrance and the exit of the microchannels. The depth of the channels and the size of the particles were not found to exert any major influence on the separation process. However, it was found that the flow rate affected the separation results, as the best results were obtained for a flow rate of 100 μL/min. Though these results are promising, further analyses and optimizations of microfluidic devices, as well as comparisons between devices sealed using different methods such as the solvent bonding technique, will be conducted in future works. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-16 2021-04-16T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/75818 |
url |
http://hdl.handle.net/1822/75818 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2673-4591 10.3390/Micromachines2021-09599 https://www.mdpi.com/2673-4591/4/1/37 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132347065434112 |