HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase

Detalhes bibliográficos
Autor(a) principal: Margalha, Leonor
Data de Publicação: 2023
Outros Autores: Elias, Alexandre, Belda-Palazón, Borja, Peixoto, Bruno, Confraria, Ana, Baena-González, Elena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158665
Resumo: Funding Information: We thank Vera Nunes from the IGC Model Organism Unit/Plant Facility for excellent plant management and Filip Rolland for kindly providing the and lines. This work was supported by FCT ‐ Fundação para a Ciência e a Tecnologia, I.P., through GREEN‐IT ‐ Bioresources for Sustainability R&D Unit (UIDB/04551/2020, UIDP/04551/2020), by the LS4FUTURE Associated Laboratory (LA/P/0087/2020, PTDC/BIA‐FBT/4942/2020, LISBOA‐01‐0145‐FEDER‐028128, PTDC/BIA‐BID/32347/2017, EXPL/ASP‐AGR/1329/2021, 2022.08339.PTDC, SFRH/BPD/116116/2016 [LM], PD/BD/114361/2016 [BP], and 2020.03177.CEECIND [EBG]), and by the European Union Horizon 2020 research and innovation program (Grant Agreement number: 867426 — ABA GrowthBalance — H2020‐WF‐2018‐2020/H2020‐WF‐01‐2018, awarded to BBP). NLS‐SnRK1α1 βMYR‐SnRK1α1 Publisher Copyright: © 2023 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
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spelling HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinaseArabidopsis thalianaHOS1low-energy stressnuclear pore complexNUP160SnRK1GeneticsPlant ScienceCell BiologyFunding Information: We thank Vera Nunes from the IGC Model Organism Unit/Plant Facility for excellent plant management and Filip Rolland for kindly providing the and lines. This work was supported by FCT ‐ Fundação para a Ciência e a Tecnologia, I.P., through GREEN‐IT ‐ Bioresources for Sustainability R&D Unit (UIDB/04551/2020, UIDP/04551/2020), by the LS4FUTURE Associated Laboratory (LA/P/0087/2020, PTDC/BIA‐FBT/4942/2020, LISBOA‐01‐0145‐FEDER‐028128, PTDC/BIA‐BID/32347/2017, EXPL/ASP‐AGR/1329/2021, 2022.08339.PTDC, SFRH/BPD/116116/2016 [LM], PD/BD/114361/2016 [BP], and 2020.03177.CEECIND [EBG]), and by the European Union Horizon 2020 research and innovation program (Grant Agreement number: 867426 — ABA GrowthBalance — H2020‐WF‐2018‐2020/H2020‐WF‐01‐2018, awarded to BBP). NLS‐SnRK1α1 βMYR‐SnRK1α1 Publisher Copyright: © 2023 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.Plants need to integrate internal and environmental signals to mount adequate stress responses. The NUCLEAR PORE COMPLEX (NPC) component HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1 (HOS1) is emerging as such an integrator, affecting responses to cold, heat, light, and salinity. Stress conditions often converge in a low-energy signal that activates SUCROSE NON-FERMENTING 1-RELATED KINASE 1 (SnRK1) to promote stress tolerance and survival. Here, we explored the role of HOS1 in the SnRK1-dependent response to low-energy stress in Arabidopsis thaliana, using darkness as a treatment and a combination of genetic, biochemical, and phenotypic assays. We show that the induction of starvation genes and plant tolerance to prolonged darkness are defective in the hos1 mutant. HOS1 interacts physically with the SnRK1α1 catalytic subunit in yeast two-hybrid assays and in planta, and the nuclear accumulation of SnRK1α1 is reduced in the hos1 mutant. Likewise, another NPC mutant, nup160, exhibits lower activation of starvation genes and decreased tolerance to prolonged darkness. Importantly, defects in low-energy responses in the hos1 background are rescued by fusing SnRK1α1 to a potent nuclear localization signal or by sugar supplementation during the dark treatment. Altogether, this work demonstrates the importance of HOS1 for the nuclear accumulation of SnRK1α1, which is key for plant tolerance to low-energy conditions.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNMargalha, LeonorElias, AlexandreBelda-Palazón, BorjaPeixoto, BrunoConfraria, AnaBaena-González, Elena2023-10-03T22:20:01Z2023-082023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/158665eng0960-7412PURE: 72982744https://doi.org/10.1111/tpj.16250info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:13Zoai:run.unl.pt:10362/158665Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:15.888749Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase
title HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase
spellingShingle HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase
Margalha, Leonor
Arabidopsis thaliana
HOS1
low-energy stress
nuclear pore complex
NUP160
SnRK1
Genetics
Plant Science
Cell Biology
title_short HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase
title_full HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase
title_fullStr HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase
title_full_unstemmed HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase
title_sort HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase
author Margalha, Leonor
author_facet Margalha, Leonor
Elias, Alexandre
Belda-Palazón, Borja
Peixoto, Bruno
Confraria, Ana
Baena-González, Elena
author_role author
author2 Elias, Alexandre
Belda-Palazón, Borja
Peixoto, Bruno
Confraria, Ana
Baena-González, Elena
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Margalha, Leonor
Elias, Alexandre
Belda-Palazón, Borja
Peixoto, Bruno
Confraria, Ana
Baena-González, Elena
dc.subject.por.fl_str_mv Arabidopsis thaliana
HOS1
low-energy stress
nuclear pore complex
NUP160
SnRK1
Genetics
Plant Science
Cell Biology
topic Arabidopsis thaliana
HOS1
low-energy stress
nuclear pore complex
NUP160
SnRK1
Genetics
Plant Science
Cell Biology
description Funding Information: We thank Vera Nunes from the IGC Model Organism Unit/Plant Facility for excellent plant management and Filip Rolland for kindly providing the and lines. This work was supported by FCT ‐ Fundação para a Ciência e a Tecnologia, I.P., through GREEN‐IT ‐ Bioresources for Sustainability R&D Unit (UIDB/04551/2020, UIDP/04551/2020), by the LS4FUTURE Associated Laboratory (LA/P/0087/2020, PTDC/BIA‐FBT/4942/2020, LISBOA‐01‐0145‐FEDER‐028128, PTDC/BIA‐BID/32347/2017, EXPL/ASP‐AGR/1329/2021, 2022.08339.PTDC, SFRH/BPD/116116/2016 [LM], PD/BD/114361/2016 [BP], and 2020.03177.CEECIND [EBG]), and by the European Union Horizon 2020 research and innovation program (Grant Agreement number: 867426 — ABA GrowthBalance — H2020‐WF‐2018‐2020/H2020‐WF‐01‐2018, awarded to BBP). NLS‐SnRK1α1 βMYR‐SnRK1α1 Publisher Copyright: © 2023 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
publishDate 2023
dc.date.none.fl_str_mv 2023-10-03T22:20:01Z
2023-08
2023-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158665
url http://hdl.handle.net/10362/158665
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0960-7412
PURE: 72982744
https://doi.org/10.1111/tpj.16250
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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