Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions

Detalhes bibliográficos
Autor(a) principal: Moço, Mariana Isabel Barata
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/151750
Resumo: Human induced pluripotent stem cells (hiPSCs) have the unique potential of self-renewal and the functional capacity to give rise to any specialized cell type in the human body, including all types of renal cells. By virtue of these outstanding features, hiPSCs constitute a powerful research tool and present an immensurable clinical potential. Kidney organoids derived from hiPSCs constitute attractive three-dimensional (3D) models that may enable kidney regeneration, disease modelling and drug screening, amongst others. The following experimental work focused on developing kidney organoids from hiPSCs under 3D conditions resorting to commercially available high-throughput microwells. For that purpose, two relevant protocols, which combine monolayer and 3D culture, were applied and adapted to a full 3D culture configuration. The conducted differentiation experiments under 3D conditions revealed temporal discrepancy of the obtained cellular stages in relation to the referred protocols. In the end, it was possible to generate immature kidney organoids.
id RCAP_34e6eef1405c212a9949907d62d13a34
oai_identifier_str oai:run.unl.pt:10362/151750
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditionsHuman induced pluripotent stem cellsRenal differentiationKidney organoids3D cultureDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasHuman induced pluripotent stem cells (hiPSCs) have the unique potential of self-renewal and the functional capacity to give rise to any specialized cell type in the human body, including all types of renal cells. By virtue of these outstanding features, hiPSCs constitute a powerful research tool and present an immensurable clinical potential. Kidney organoids derived from hiPSCs constitute attractive three-dimensional (3D) models that may enable kidney regeneration, disease modelling and drug screening, amongst others. The following experimental work focused on developing kidney organoids from hiPSCs under 3D conditions resorting to commercially available high-throughput microwells. For that purpose, two relevant protocols, which combine monolayer and 3D culture, were applied and adapted to a full 3D culture configuration. The conducted differentiation experiments under 3D conditions revealed temporal discrepancy of the obtained cellular stages in relation to the referred protocols. In the end, it was possible to generate immature kidney organoids.As células estaminais pluripotentes induzidas humanas (hiPSCs) têm o potencial único de autorrenovação e a capacidade funcional de originar qualquer tipo de célula especializada no corpo humano, incluindo todos os tipos de células renais. Em virtude destas características excecionais, as hiPSCs são um poderoso instrumento de pesquisa e apresentam um potencial clínico imensurável. Os organóides de rim derivados de hiPSCs constituem modelos tridimensionais (3D) atrativos que podem permitir a regeneração do rim, modelação de doenças e triagem de fármacos, entre outros. O seguinte trabalho experimental focou-se no desenvolvimento de organóides de rim a partir de hiPSCs sob condições 3D recorrendo a uma metodologia high-throughput através do uso de micropoços. Com esse propósito, dois protocolos relevantes, que combinam cultura em condições aderentes e cultura 3D, foram aplicados e adaptados a uma configuração de cultura totalmente em condições 3D. As experiências de diferenciação conduzidas sob condições 3D revelaram uma discrepância temporal dos estágios celulares obtidos em relação aos protocolos referidos. No final, foi possível gerar organóides de rim imaturos.Simões, CláudiaRUNMoço, Mariana Isabel Barata2023-04-12T11:48:24Z2022-022022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/151750enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:34:07Zoai:run.unl.pt:10362/151750Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:41.069728Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions
title Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions
spellingShingle Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions
Moço, Mariana Isabel Barata
Human induced pluripotent stem cells
Renal differentiation
Kidney organoids
3D culture
Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias
title_short Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions
title_full Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions
title_fullStr Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions
title_full_unstemmed Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions
title_sort Derivation of kidney organoids from human induced pluripotent stem cells under 3D conditions
author Moço, Mariana Isabel Barata
author_facet Moço, Mariana Isabel Barata
author_role author
dc.contributor.none.fl_str_mv Simões, Cláudia
RUN
dc.contributor.author.fl_str_mv Moço, Mariana Isabel Barata
dc.subject.por.fl_str_mv Human induced pluripotent stem cells
Renal differentiation
Kidney organoids
3D culture
Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias
topic Human induced pluripotent stem cells
Renal differentiation
Kidney organoids
3D culture
Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias
description Human induced pluripotent stem cells (hiPSCs) have the unique potential of self-renewal and the functional capacity to give rise to any specialized cell type in the human body, including all types of renal cells. By virtue of these outstanding features, hiPSCs constitute a powerful research tool and present an immensurable clinical potential. Kidney organoids derived from hiPSCs constitute attractive three-dimensional (3D) models that may enable kidney regeneration, disease modelling and drug screening, amongst others. The following experimental work focused on developing kidney organoids from hiPSCs under 3D conditions resorting to commercially available high-throughput microwells. For that purpose, two relevant protocols, which combine monolayer and 3D culture, were applied and adapted to a full 3D culture configuration. The conducted differentiation experiments under 3D conditions revealed temporal discrepancy of the obtained cellular stages in relation to the referred protocols. In the end, it was possible to generate immature kidney organoids.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
2022-02-01T00:00:00Z
2023-04-12T11:48:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/151750
url http://hdl.handle.net/10362/151750
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138135328686080

Registros relacionados