Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/62451 |
Resumo: | Background: The pathogenesis of lung cancer is triggered by a combination of genetic and environmental factors, being the tobacco smoke the most important risk factor. Nevertheless, the incidence of lung cancer in non-smokers is gradually increasing, which demands the search for different other etiological factors such as occupational exposure, previous lung disease, diet among others. In the early 80's a theory linked specific types of human papillomavirus (HPV) to lung cancer due to morphological similarities of a subset of bronchial squamous cell carcinomas with other HPV-induced cancers. Since then, several studies revealed variable rates of HPV DNA detection. The current study aimed to provide accurate information on the prevalence of HPV DNA in lung cancer. Methods: Biopsies were collected from 77 newly diagnosed non-small cell lung cancer (NSCLC) patients treated at the Thoracic Oncology Department at Barretos Cancer Hospital. The samples were formalin fixed and paraffin embedded (FFPE), histologic analysis was performed by an experienced pathologist. DNA was extracted from FFPE material using a commercial extraction kit and HPV DNA detection was evaluated by multiplex PCR and HPV16 specific real-time PCR. Results: HPV was not identified in any of the samples analysed (69). Conclusions: Our data demonstrated a lack of HPV DNA in a series of NSCL cancers. |
id |
RCAP_356255437097ec46b8ff2f4542fae52d |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/62451 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional studyNon-small cell lung cancerLung neoplasmsPapillomaviridaePapillomavirus DNAScience & TechnologyBackground: The pathogenesis of lung cancer is triggered by a combination of genetic and environmental factors, being the tobacco smoke the most important risk factor. Nevertheless, the incidence of lung cancer in non-smokers is gradually increasing, which demands the search for different other etiological factors such as occupational exposure, previous lung disease, diet among others. In the early 80's a theory linked specific types of human papillomavirus (HPV) to lung cancer due to morphological similarities of a subset of bronchial squamous cell carcinomas with other HPV-induced cancers. Since then, several studies revealed variable rates of HPV DNA detection. The current study aimed to provide accurate information on the prevalence of HPV DNA in lung cancer. Methods: Biopsies were collected from 77 newly diagnosed non-small cell lung cancer (NSCLC) patients treated at the Thoracic Oncology Department at Barretos Cancer Hospital. The samples were formalin fixed and paraffin embedded (FFPE), histologic analysis was performed by an experienced pathologist. DNA was extracted from FFPE material using a commercial extraction kit and HPV DNA detection was evaluated by multiplex PCR and HPV16 specific real-time PCR. Results: HPV was not identified in any of the samples analysed (69). Conclusions: Our data demonstrated a lack of HPV DNA in a series of NSCL cancers.This work was funded by Conselho Nacional de Desenvolvimento Científico e Tencnológico (CNPq) [Grants number 401775/2012–7 to ALF; 573799/ 2008–3 to LLV]; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) [Grants numbers 2014/23414–8 to EMS; 08/57889–1 to LLV].BioMed Central (BMC)Universidade do MinhoSilva, Estela MariaMariano, Vânia SammartinoPastrez, Paula Roberta AguiarPinto, Miguel CordobaNunes, Emily MontosaSichero, LauraVilla, Luisa LinaScapulatempo-Neto, CristovamSyrjanen, Kari JuhaniLongatto, Adhemar20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62451eng1750-937810.1186/s13027-019-0235-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:34Zoai:repositorium.sdum.uminho.pt:1822/62451Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:35.986655Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study |
title |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study |
spellingShingle |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study Silva, Estela Maria Non-small cell lung cancer Lung neoplasms Papillomaviridae Papillomavirus DNA Science & Technology |
title_short |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study |
title_full |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study |
title_fullStr |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study |
title_full_unstemmed |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study |
title_sort |
Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study |
author |
Silva, Estela Maria |
author_facet |
Silva, Estela Maria Mariano, Vânia Sammartino Pastrez, Paula Roberta Aguiar Pinto, Miguel Cordoba Nunes, Emily Montosa Sichero, Laura Villa, Luisa Lina Scapulatempo-Neto, Cristovam Syrjanen, Kari Juhani Longatto, Adhemar |
author_role |
author |
author2 |
Mariano, Vânia Sammartino Pastrez, Paula Roberta Aguiar Pinto, Miguel Cordoba Nunes, Emily Montosa Sichero, Laura Villa, Luisa Lina Scapulatempo-Neto, Cristovam Syrjanen, Kari Juhani Longatto, Adhemar |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Estela Maria Mariano, Vânia Sammartino Pastrez, Paula Roberta Aguiar Pinto, Miguel Cordoba Nunes, Emily Montosa Sichero, Laura Villa, Luisa Lina Scapulatempo-Neto, Cristovam Syrjanen, Kari Juhani Longatto, Adhemar |
dc.subject.por.fl_str_mv |
Non-small cell lung cancer Lung neoplasms Papillomaviridae Papillomavirus DNA Science & Technology |
topic |
Non-small cell lung cancer Lung neoplasms Papillomaviridae Papillomavirus DNA Science & Technology |
description |
Background: The pathogenesis of lung cancer is triggered by a combination of genetic and environmental factors, being the tobacco smoke the most important risk factor. Nevertheless, the incidence of lung cancer in non-smokers is gradually increasing, which demands the search for different other etiological factors such as occupational exposure, previous lung disease, diet among others. In the early 80's a theory linked specific types of human papillomavirus (HPV) to lung cancer due to morphological similarities of a subset of bronchial squamous cell carcinomas with other HPV-induced cancers. Since then, several studies revealed variable rates of HPV DNA detection. The current study aimed to provide accurate information on the prevalence of HPV DNA in lung cancer. Methods: Biopsies were collected from 77 newly diagnosed non-small cell lung cancer (NSCLC) patients treated at the Thoracic Oncology Department at Barretos Cancer Hospital. The samples were formalin fixed and paraffin embedded (FFPE), histologic analysis was performed by an experienced pathologist. DNA was extracted from FFPE material using a commercial extraction kit and HPV DNA detection was evaluated by multiplex PCR and HPV16 specific real-time PCR. Results: HPV was not identified in any of the samples analysed (69). Conclusions: Our data demonstrated a lack of HPV DNA in a series of NSCL cancers. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/62451 |
url |
http://hdl.handle.net/1822/62451 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1750-9378 10.1186/s13027-019-0235-8 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central (BMC) |
publisher.none.fl_str_mv |
BioMed Central (BMC) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132641444757504 |