Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity

Detalhes bibliográficos
Autor(a) principal: Borges, Anabela
Data de Publicação: 2014
Outros Autores: Serra, Sofia Cristina, Abreu, Ana Cristina, Saavedra, Maria J., Salgado, A. J., Simões, Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/32980
Resumo: Quorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI.
id RCAP_3a28a7a842def514e650b05cbf72aacd
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/32980
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicityBiofilmsChromobacterium violaceumCitotoxicityIsothiocyanatesPhenolicsPhytochemicalsQuorum sensing inhibitioncytotoxicityScience & TechnologyQuorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI.This work was supported by Operational Programme for Competitiveness Factors - COMPETE, FCT/MEC (PIDDAC) and FEDER through Projects Bioresist - PTDC/EBB-EBI/105085/2008; Phytodisinfectants - PTDC/DTP-SAP/1078/2012 (COMPETE: FCOMP-01-0124-FEDER-028765) and the PhD grants awarded to Ana Abreu (SFRH/BD/84393/2012) and Anabela Borges (SFRH/BD/63398/2009). The authors are also very grateful to Professor Robert McLean (Texas University, USA) for providing the bacterial strains.Taylor and FrancisUniversidade do MinhoBorges, AnabelaSerra, Sofia CristinaAbreu, Ana CristinaSaavedra, Maria J.Salgado, A. J.Simões, Manuel20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32980eng0892-701410.1080/08927014.2013.85254224344870http://dx.doi.org/10.1080/08927014.2013.852542info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:08Zoai:repositorium.sdum.uminho.pt:1822/32980Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:34:30.997511Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
title Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
spellingShingle Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
Borges, Anabela
Biofilms
Chromobacterium violaceum
Citotoxicity
Isothiocyanates
Phenolics
Phytochemicals
Quorum sensing inhibition
cytotoxicity
Science & Technology
title_short Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
title_full Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
title_fullStr Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
title_full_unstemmed Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
title_sort Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
author Borges, Anabela
author_facet Borges, Anabela
Serra, Sofia Cristina
Abreu, Ana Cristina
Saavedra, Maria J.
Salgado, A. J.
Simões, Manuel
author_role author
author2 Serra, Sofia Cristina
Abreu, Ana Cristina
Saavedra, Maria J.
Salgado, A. J.
Simões, Manuel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Borges, Anabela
Serra, Sofia Cristina
Abreu, Ana Cristina
Saavedra, Maria J.
Salgado, A. J.
Simões, Manuel
dc.subject.por.fl_str_mv Biofilms
Chromobacterium violaceum
Citotoxicity
Isothiocyanates
Phenolics
Phytochemicals
Quorum sensing inhibition
cytotoxicity
Science & Technology
topic Biofilms
Chromobacterium violaceum
Citotoxicity
Isothiocyanates
Phenolics
Phytochemicals
Quorum sensing inhibition
cytotoxicity
Science & Technology
description Quorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/32980
url http://hdl.handle.net/1822/32980
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0892-7014
10.1080/08927014.2013.852542
24344870
http://dx.doi.org/10.1080/08927014.2013.852542
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132867712778240

Registros relacionados