Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/7700 |
Resumo: | Siboglinids are tube-dwelling annelids that are important members of deep-sea chemosynthetic communities, which include hydrothermal vents, cold seeps, whale-falls and reduced sediments. As adults, they lack a functional digestive system and rely on microbial endosymbionts for their energetic needs. Currently most researchers recognize four main lineages within Siboglinidae, Frenulata, Vestimentifera, Sclerolinum and Osedax, of which Frenulata is the most understudied. The discovery of chemosynthetic-based communities dominated by frenulates in several mud volcanoes in the Gulf of Cadiz, and the possibility to explore these habitats through several research programmes has been an excellent opportunity to extend our knowledge on these fascinating tubeworms. As other chemosymbiotic animals, frenulates have depleted values of δ13C that result from the metabolic pathways of their associated bacteria. In this study, stable isotope signatures were used to determine (1) the energy source (methane or sulphur) of the endosymbionts of different species of frenulates, (2) the occurrence of resource partitioning between species inhabiting the same site and (3) differences in the energy sources of the same species inhabiting different sites. Additionally, the effect of different sample preservation methodologies (frozen, ethanol, and formaldehyde) in the isotopic signatures was also compared. The δ13C values of both species from Porto mud volcano indicate that these species may host methanotrophic symbionts and that they are able to exploit different microhabitats within the same seep site. The carbon isotopic signatures were more (Spirobrachia tripeira and Lamellisabella denticulata) or less (Siboglinum cf. poseidoni) depleted than expected suggesting that dual symbiosis may be more prevalent than previously though in frenulates. It is hypothesized that dual symbioses allow frenulates to survive in the spatially and temporally variable chemical environment of mud volcanoes. The effects of the preservation method on δ13C and δ15N values is in agreement with previous studies and suggest that frenulate samples preserved chemically can be used in trophic ecology studies. However results obtained with samples including the chitinous tube of the animals should be interpreted with caution. |
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Isotopic signatures of Frenulata from cold seeps in the Gulf of CadizBiologia marinhaAnelídeosMacrofauna bentónicaVulcões de lama - Golfo de CádizProcessos hidrotermaisIsótopos estáveisSiboglinids are tube-dwelling annelids that are important members of deep-sea chemosynthetic communities, which include hydrothermal vents, cold seeps, whale-falls and reduced sediments. As adults, they lack a functional digestive system and rely on microbial endosymbionts for their energetic needs. Currently most researchers recognize four main lineages within Siboglinidae, Frenulata, Vestimentifera, Sclerolinum and Osedax, of which Frenulata is the most understudied. The discovery of chemosynthetic-based communities dominated by frenulates in several mud volcanoes in the Gulf of Cadiz, and the possibility to explore these habitats through several research programmes has been an excellent opportunity to extend our knowledge on these fascinating tubeworms. As other chemosymbiotic animals, frenulates have depleted values of δ13C that result from the metabolic pathways of their associated bacteria. In this study, stable isotope signatures were used to determine (1) the energy source (methane or sulphur) of the endosymbionts of different species of frenulates, (2) the occurrence of resource partitioning between species inhabiting the same site and (3) differences in the energy sources of the same species inhabiting different sites. Additionally, the effect of different sample preservation methodologies (frozen, ethanol, and formaldehyde) in the isotopic signatures was also compared. The δ13C values of both species from Porto mud volcano indicate that these species may host methanotrophic symbionts and that they are able to exploit different microhabitats within the same seep site. The carbon isotopic signatures were more (Spirobrachia tripeira and Lamellisabella denticulata) or less (Siboglinum cf. poseidoni) depleted than expected suggesting that dual symbiosis may be more prevalent than previously though in frenulates. It is hypothesized that dual symbioses allow frenulates to survive in the spatially and temporally variable chemical environment of mud volcanoes. The effects of the preservation method on δ13C and δ15N values is in agreement with previous studies and suggest that frenulate samples preserved chemically can be used in trophic ecology studies. However results obtained with samples including the chitinous tube of the animals should be interpreted with caution.Os Siboglinideos são anelídeos tubícolares, componentes importantes de comunidades quimiossintéticas profundas como fontes hidrotermais, fontes frias, carcaças de baleias e sedimentos reduzidos. Em adultos, estes poliqueta não possuem um sistema digestivo funcional, sendo dependentes de endossimbiontes microbianos para as suas necessidades energéticas. Atualmente são reconhecidas quatro linhagens principais dentro da familia Siboglinidae: Frenulata, Vestimentifera, Sclerolinum e Osedax, dos quais os Frenulata são os menos estudados. A descoberta de comunidades quimiossintéticas dominadas por Frenulata em vários vulcões de lama no Golfo de Cádis e a possibilidade de explorar estes ecossistemas através de diversos programas de pesquisa tem sido uma boa oportunidade para expandir o conhecimentos neste fascinate grupo de animais. Como outros animais quimiossintéticos, os Frenulata tem valores de δ13C empobrecidos resultantes das vias metabólicas das suas bactérias associadas. Neste estudo as assinaturas de isótopos estáveis foram utilizadas para determinar (1) a fonte de energia (metano ou enxofre) das bactérias endossimbiontes das diferentes espécies de Frenulata, (2) a ocorrência de partição de recursos entre espécies que habitam o mesmo local e (3) diferenças nas fontes de energia da mesma espécie proveniente de diferentes locais. Adicionalmente os efeitos de diferentes métodos de conservação (congelação, álcool e formaldeído) nas assinaturas isotópicas foram comparados. O valor de δ13C de ambas as espécies colhidas no vulcão de lama Porto sugere que as mesmas podem hospedar bactérias metanotróficas, e que são capazes de explorar microhabitats diferentes num mesmo vulcão de lama. As assinaturas isotópicas de carbono foram mais (Spirobrachia tripeira e Lamelisabella denticulata) ou menos (Siboglinum cf. poseidoni) baixas do que o esperado sugerindo a ocorrência de uma simbiose dupla, sugerindo que este tipo de simbiose pode ser mais frequente do que se pensava em Frenulata. Hipoteticamente a simbiose dupla pode permitir aos Frenulata sobreviver em ambientes químicos temporal e espacialmente variáveis como é o caso dos vulcões de lama. Os efeitos dos métodos de preservação nos valores de δ13C e δ15N estão de acordo com estudos anteriores, sugerindo que amostras de Frenulata preservadas quimicamente podem ser utilizadas para estudos de ecologia trófica. Porém, os resultados obtidos para amostras que incluem o tubo de quitina característico destes animais devem ser interpretados com cautela.Universidade de Aveiro2018-07-20T14:00:36Z2011-12-14T00:00:00Z2011-12-142013-12-07T17:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/7700engCarramaschi, Guilherme Leiteinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:13:25Zoai:ria.ua.pt:10773/7700Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:45:19.780275Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz |
title |
Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz |
spellingShingle |
Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz Carramaschi, Guilherme Leite Biologia marinha Anelídeos Macrofauna bentónica Vulcões de lama - Golfo de Cádiz Processos hidrotermais Isótopos estáveis |
title_short |
Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz |
title_full |
Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz |
title_fullStr |
Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz |
title_full_unstemmed |
Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz |
title_sort |
Isotopic signatures of Frenulata from cold seeps in the Gulf of Cadiz |
author |
Carramaschi, Guilherme Leite |
author_facet |
Carramaschi, Guilherme Leite |
author_role |
author |
dc.contributor.author.fl_str_mv |
Carramaschi, Guilherme Leite |
dc.subject.por.fl_str_mv |
Biologia marinha Anelídeos Macrofauna bentónica Vulcões de lama - Golfo de Cádiz Processos hidrotermais Isótopos estáveis |
topic |
Biologia marinha Anelídeos Macrofauna bentónica Vulcões de lama - Golfo de Cádiz Processos hidrotermais Isótopos estáveis |
description |
Siboglinids are tube-dwelling annelids that are important members of deep-sea chemosynthetic communities, which include hydrothermal vents, cold seeps, whale-falls and reduced sediments. As adults, they lack a functional digestive system and rely on microbial endosymbionts for their energetic needs. Currently most researchers recognize four main lineages within Siboglinidae, Frenulata, Vestimentifera, Sclerolinum and Osedax, of which Frenulata is the most understudied. The discovery of chemosynthetic-based communities dominated by frenulates in several mud volcanoes in the Gulf of Cadiz, and the possibility to explore these habitats through several research programmes has been an excellent opportunity to extend our knowledge on these fascinating tubeworms. As other chemosymbiotic animals, frenulates have depleted values of δ13C that result from the metabolic pathways of their associated bacteria. In this study, stable isotope signatures were used to determine (1) the energy source (methane or sulphur) of the endosymbionts of different species of frenulates, (2) the occurrence of resource partitioning between species inhabiting the same site and (3) differences in the energy sources of the same species inhabiting different sites. Additionally, the effect of different sample preservation methodologies (frozen, ethanol, and formaldehyde) in the isotopic signatures was also compared. The δ13C values of both species from Porto mud volcano indicate that these species may host methanotrophic symbionts and that they are able to exploit different microhabitats within the same seep site. The carbon isotopic signatures were more (Spirobrachia tripeira and Lamellisabella denticulata) or less (Siboglinum cf. poseidoni) depleted than expected suggesting that dual symbiosis may be more prevalent than previously though in frenulates. It is hypothesized that dual symbioses allow frenulates to survive in the spatially and temporally variable chemical environment of mud volcanoes. The effects of the preservation method on δ13C and δ15N values is in agreement with previous studies and suggest that frenulate samples preserved chemically can be used in trophic ecology studies. However results obtained with samples including the chitinous tube of the animals should be interpreted with caution. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-14T00:00:00Z 2011-12-14 2013-12-07T17:00:00Z 2018-07-20T14:00:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/7700 |
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http://hdl.handle.net/10773/7700 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de Aveiro |
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Universidade de Aveiro |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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