CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis

Detalhes bibliográficos
Autor(a) principal: Fabre-Mersseman, Véronique
Data de Publicação: 2011
Outros Autores: Dutrieux, Jacques, Louise, Anne, Rozlan, Sandra, Lamine, Aurélia, Parker, Raphaëlle, Rancez, Magali, Nunes-Cabaço, Helena, Sousa, Ana E., Lambotte, Olivier, Cheynier, Rémi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/20112
Resumo: © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins
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spelling CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesisHIVHIV pathogenesisHIV reservoirInterleukin 7Naive CD4+ T cellsThymus© 2011 Wolters Kluwer Health | Lippincott Williams & WilkinsOBJECTIVE: The contribution of naive CD4+ T cells to the pool of HIV-infected cells remains poorly described. This study aimed at evaluating HIV infection in naive T-cell subsets in viremic and HAART-treated patients, together with various parameters implicated in naive T-cell homeostasis, in order to better understand infection in these subsets. DESIGN AND METHODS: HIV provirus was quantified in various FACS-sorted CD4/CD8 T-cell subsets [recent thymic emigrants (RTEs), non-RTE naives and memory T cells] purified from peripheral blood cells of untreated viremic and HAART-treated aviremic HIV-infected patients. HIV proviral DNA was quantified using a highly sensitive real-time PCR assay allowing detection of one HIV copy in 10⁵ cells. Intrathymic precursor T-cell proliferation and circulating T-cell cycling were, respectively, evaluated through measurement of the sj/βTREC ratio (signal joint T-Cell Receptor Excision Circle frequency divided by DβJβTREC frequency) and Ki-67 expression. Plasma interleukin (IL)-7 concentrations were measured by ELISA. RESULTS: RTEs and non-RTEs were equally HIV infected. Altogether, naive CD4+ T cells represented 0.24%-60% of the infected cells. In contrast, HIV DNA was undetectable in naive CD8+ T cells. RTE infection rate directly correlated with IL-7 plasma levels (r = 0.607, P = 0.0035) but was independent from plasma viral load, peripheral T-cell cycling and intrathymic precursor T-cell proliferation. CONCLUSION: We demonstrated that RTEs are effectively HIV infected. The similar infection rate observed in RTEs and other naive T cells, its relationship with plasma IL-7 levels, together with the lack of correlation between RTE infection and either thymic or peripheral proliferation, strongly suggests that RTE infection occurs either late during thymopoiesis or early on during their extrathymic maturation.Lippincott, Williams & Wilkins / Wolters Kluwer HealthRepositório da Universidade de LisboaFabre-Mersseman, VéroniqueDutrieux, JacquesLouise, AnneRozlan, SandraLamine, AuréliaParker, RaphaëlleRancez, MagaliNunes-Cabaço, HelenaSousa, Ana E.Lambotte, OlivierCheynier, Rémi2015-09-22T10:43:52Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/20112engAIDS 2011, 25:1153–11620269-9370http://dx.doi.org/10.1097/QAD.0b013e3283471e89metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:05:12Zoai:repositorio.ul.pt:10451/20112Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:38:12.525844Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis
title CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis
spellingShingle CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis
Fabre-Mersseman, Véronique
HIV
HIV pathogenesis
HIV reservoir
Interleukin 7
Naive CD4+ T cells
Thymus
title_short CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis
title_full CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis
title_fullStr CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis
title_full_unstemmed CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis
title_sort CD4+ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis
author Fabre-Mersseman, Véronique
author_facet Fabre-Mersseman, Véronique
Dutrieux, Jacques
Louise, Anne
Rozlan, Sandra
Lamine, Aurélia
Parker, Raphaëlle
Rancez, Magali
Nunes-Cabaço, Helena
Sousa, Ana E.
Lambotte, Olivier
Cheynier, Rémi
author_role author
author2 Dutrieux, Jacques
Louise, Anne
Rozlan, Sandra
Lamine, Aurélia
Parker, Raphaëlle
Rancez, Magali
Nunes-Cabaço, Helena
Sousa, Ana E.
Lambotte, Olivier
Cheynier, Rémi
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Fabre-Mersseman, Véronique
Dutrieux, Jacques
Louise, Anne
Rozlan, Sandra
Lamine, Aurélia
Parker, Raphaëlle
Rancez, Magali
Nunes-Cabaço, Helena
Sousa, Ana E.
Lambotte, Olivier
Cheynier, Rémi
dc.subject.por.fl_str_mv HIV
HIV pathogenesis
HIV reservoir
Interleukin 7
Naive CD4+ T cells
Thymus
topic HIV
HIV pathogenesis
HIV reservoir
Interleukin 7
Naive CD4+ T cells
Thymus
description © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2015-09-22T10:43:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/20112
url http://hdl.handle.net/10451/20112
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv AIDS 2011, 25:1153–1162
0269-9370
http://dx.doi.org/10.1097/QAD.0b013e3283471e89
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Lippincott, Williams & Wilkins / Wolters Kluwer Health
publisher.none.fl_str_mv Lippincott, Williams & Wilkins / Wolters Kluwer Health
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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