Blood flow visualization and measurements in microfluidic devices fabricated by a micromilling technique

• Autor(a) principal: Singhal, Jaron
• Data de Publicação: 2015
• Outros Autores: Pinho, Diana, Lopes, Raquel, Sousa, Patricia C., Garcia, Valdemar, Schütte, Helmut, Lima, Rui Alberto Madeira Macedo, Gassmann, Stefan
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/51097
• topic: Biomedical applications; Blood cells; Cell-free layer; Cells separation; Micromilling; Microfluidic devices

The most common and used technique to produce microfluidic devices for biomedical applications is the soft-lithography. However, this is a high cost and time-consuming technique. Recently, manufacturers were able to produce milling tools smaller than 100 m and consequently have promoted the ability of the micromilling machines to fabricate microfluidic devices capable of performing cell separation. In this work, we show the ability of a micromilling machine to manufacture microchannels down to 30 m and also the ability of a microfluidic device to perform partial separation of red blood cells from plasma. Flow visualization and measurements were performed by using a high-speed video microscopy system. Advantages and limitations of the micromilling fabrication process are also presented.