Ultrasound-assisted biosynthesis of novel methotrexate-conjugates
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/55082 |
Resumo: | New methotrexate-acylglycerols and methotrexate-cyclodextrins (, and -CD) conjugates were obtained via esterification or transesterification reactions. All reactions were catalysed by esterases namely immobilized Lipase from Candida antarctica B and Lipase from Thermomyces lanuginosus. The use of ultrasound to assist the reactions revealed to be a key factor to obtain high conversion yields on both MTX conjugates. Transesterification reactions including long chain triacylglycerols were only successful when ultrasound was applied. In cyclodextrins esterification a higher number of MTX molecules was also linked to cyclodextrins when ultrasound was used. All the conjugates were characterized by MALDI-TOF and NMR spectroscopy. |
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Ultrasound-assisted biosynthesis of novel methotrexate-conjugatesBiosynthesisLipasesUltrasoundMethotrexatetriacylglycerolscyclodextrinsScience & TechnologyNew methotrexate-acylglycerols and methotrexate-cyclodextrins (, and -CD) conjugates were obtained via esterification or transesterification reactions. All reactions were catalysed by esterases namely immobilized Lipase from Candida antarctica B and Lipase from Thermomyces lanuginosus. The use of ultrasound to assist the reactions revealed to be a key factor to obtain high conversion yields on both MTX conjugates. Transesterification reactions including long chain triacylglycerols were only successful when ultrasound was applied. In cyclodextrins esterification a higher number of MTX molecules was also linked to cyclodextrins when ultrasound was used. All the conjugates were characterized by MALDI-TOF and NMR spectroscopy.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Jennifer Noro also thanks to FCT – Fundação para a Ciência e a Tecnologia for funding her scholarship (SFRH/BD/121673/2016). This work has also received funding from the European Union Horizon 2020 research and innovation programme under grant agreement NMP-06-2015-683356 FOLSMART.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoNoro, Jennifer MartinsReis, R. L.Cavaco-Paulo, ArturSilva, Carla2018-112018-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/55082engJennifer Noro; Reis, Rui L.; Cavaco-Paulo, Artur; Silva, Carla, Ultrasound-assisted biosynthesis of novel methotrexate-conjugates. Ultrasonics Sonochemistry, 48, 51-56, 20181350-41771350-417710.1016/j.ultsonch.2018.05.01730080579http://www.journals.elsevier.com/ultrasonics-sonochemistry/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:37Zoai:repositorium.sdum.uminho.pt:1822/55082Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:50:32.982609Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ultrasound-assisted biosynthesis of novel methotrexate-conjugates |
title |
Ultrasound-assisted biosynthesis of novel methotrexate-conjugates |
spellingShingle |
Ultrasound-assisted biosynthesis of novel methotrexate-conjugates Noro, Jennifer Martins Biosynthesis Lipases Ultrasound Methotrexate triacylglycerols cyclodextrins Science & Technology |
title_short |
Ultrasound-assisted biosynthesis of novel methotrexate-conjugates |
title_full |
Ultrasound-assisted biosynthesis of novel methotrexate-conjugates |
title_fullStr |
Ultrasound-assisted biosynthesis of novel methotrexate-conjugates |
title_full_unstemmed |
Ultrasound-assisted biosynthesis of novel methotrexate-conjugates |
title_sort |
Ultrasound-assisted biosynthesis of novel methotrexate-conjugates |
author |
Noro, Jennifer Martins |
author_facet |
Noro, Jennifer Martins Reis, R. L. Cavaco-Paulo, Artur Silva, Carla |
author_role |
author |
author2 |
Reis, R. L. Cavaco-Paulo, Artur Silva, Carla |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Noro, Jennifer Martins Reis, R. L. Cavaco-Paulo, Artur Silva, Carla |
dc.subject.por.fl_str_mv |
Biosynthesis Lipases Ultrasound Methotrexate triacylglycerols cyclodextrins Science & Technology |
topic |
Biosynthesis Lipases Ultrasound Methotrexate triacylglycerols cyclodextrins Science & Technology |
description |
New methotrexate-acylglycerols and methotrexate-cyclodextrins (, and -CD) conjugates were obtained via esterification or transesterification reactions. All reactions were catalysed by esterases namely immobilized Lipase from Candida antarctica B and Lipase from Thermomyces lanuginosus. The use of ultrasound to assist the reactions revealed to be a key factor to obtain high conversion yields on both MTX conjugates. Transesterification reactions including long chain triacylglycerols were only successful when ultrasound was applied. In cyclodextrins esterification a higher number of MTX molecules was also linked to cyclodextrins when ultrasound was used. All the conjugates were characterized by MALDI-TOF and NMR spectroscopy. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11 2018-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/55082 |
url |
http://hdl.handle.net/1822/55082 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Jennifer Noro; Reis, Rui L.; Cavaco-Paulo, Artur; Silva, Carla, Ultrasound-assisted biosynthesis of novel methotrexate-conjugates. Ultrasonics Sonochemistry, 48, 51-56, 2018 1350-4177 1350-4177 10.1016/j.ultsonch.2018.05.017 30080579 http://www.journals.elsevier.com/ultrasonics-sonochemistry/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133090815148032 |