A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://doi.org/10.1038/s41598-019-39209-y |
Resumo: | This work was supported by the Applied Molecular Biosciences Unit-UCIBIO which is financed by national funds from FCT/MCTES (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). C.M.S. and T.M. thank the financial support from Fundacao para a Ciencia e Tecnologia (Fellowships SFRH/BPD/79566/2011 and PD/BD/109687/2015). |
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oai:run.unl.pt:10362/76534 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanideGeneralSDG 3 - Good Health and Well-beingThis work was supported by the Applied Molecular Biosciences Unit-UCIBIO which is financed by national funds from FCT/MCTES (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). C.M.S. and T.M. thank the financial support from Fundacao para a Ciencia e Tecnologia (Fellowships SFRH/BPD/79566/2011 and PD/BD/109687/2015).The ubiquitous nitrite is a major analyte in the management of human health and environmental risks. The current analytical methods are complex techniques that do not fulfil the need for simple, robust and low-cost tools for on-site monitoring. Electrochemical reductase-based biosensors are presented as a powerful alternative, due to their good analytical performance and miniaturization potential. However, their real-world application is limited by the need of anoxic working conditions, and the standard oxygen removal strategies are incompatible with point-of-care measurements. Instead, a bienzymatic oxygen scavenger system comprising glucose oxidase and catalase can be used to promote anoxic conditions in aired environments. Herein, carbon screen-printed electrodes were modified with cytochrome c nitrite reductase together with glucose oxidase and catalase, so that nitrite cathodic detection could be performed by cyclic voltammetry under ambient air. The resulting biosensor displayed good linear response to the analyte (2–200 µM, sensitivity of 326 ± 5 mA M −1 cm −2 at −0.8 V; 0.8–150 µM, sensitivity of 511 ± 11 mA M −1 cm −2 at −0.5 V), while being free from oxygen interference and stable up to 1 month. Furthermore, the biosensor’s catalytic response was unaffected by the presence of cyanide, a well-known inhibitor of heme-enzymes.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaMolecular, Structural and Cellular Microbiology (MOSTMICRO)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNMonteiro, TiagoGomes, SaraJubete, ElenaAñorga, LarraitzSilveira, Célia M.Almeida, Maria Gabriela2019-07-25T22:34:47Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41598-019-39209-yeng2045-2322PURE: 13324878http://www.scopus.com/inward/record.url?scp=85062010407&partnerID=8YFLogxKhttps://doi.org/10.1038/s41598-019-39209-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:34:51Zoai:run.unl.pt:10362/76534Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:38.473016Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide |
| title |
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide |
| spellingShingle |
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide Monteiro, Tiago General SDG 3 - Good Health and Well-being |
| title_short |
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide |
| title_full |
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide |
| title_fullStr |
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide |
| title_full_unstemmed |
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide |
| title_sort |
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide |
| author |
Monteiro, Tiago |
| author_facet |
Monteiro, Tiago Gomes, Sara Jubete, Elena Añorga, Larraitz Silveira, Célia M. Almeida, Maria Gabriela |
| author_role |
author |
| author2 |
Gomes, Sara Jubete, Elena Añorga, Larraitz Silveira, Célia M. Almeida, Maria Gabriela |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida Molecular, Structural and Cellular Microbiology (MOSTMICRO) Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
| dc.contributor.author.fl_str_mv |
Monteiro, Tiago Gomes, Sara Jubete, Elena Añorga, Larraitz Silveira, Célia M. Almeida, Maria Gabriela |
| dc.subject.por.fl_str_mv |
General SDG 3 - Good Health and Well-being |
| topic |
General SDG 3 - Good Health and Well-being |
| description |
This work was supported by the Applied Molecular Biosciences Unit-UCIBIO which is financed by national funds from FCT/MCTES (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). C.M.S. and T.M. thank the financial support from Fundacao para a Ciencia e Tecnologia (Fellowships SFRH/BPD/79566/2011 and PD/BD/109687/2015). |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-07-25T22:34:47Z 2019-12-01 2019-12-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1038/s41598-019-39209-y |
| url |
https://doi.org/10.1038/s41598-019-39209-y |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2045-2322 PURE: 13324878 http://www.scopus.com/inward/record.url?scp=85062010407&partnerID=8YFLogxK https://doi.org/10.1038/s41598-019-39209-y |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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