Phenolic compounds characterization by LC-DAD- ESI/MSn and bioactive properties of Thymus algeriensis Boiss. & Reut. and Ephedra alata Decne

• Autor(a) principal: Ziani, Borhane E.C.
• Data de Publicação: 2018
• Outros Autores: Heleno, Sandrina A., Bachari, Khaldoun, Dias, Maria Inês, Alves, Maria José, Barros, Lillian, Ferreira, Isabel C.F.R.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10198/18569
• topic: Ephedra alata; Infusion/decoction/hydroethanolic extract; Phenolic profile/bioactivities; Thymus algeriensis

Scientific research has been focused on finding natural occurring molecules from plant origin. Herein, infusion, decoction and hydroethanolic extracts of Thymus algeriensis Boiss. & Reut. and Ephedra alata Decne. from Algeria were phytochemically characterized by LC-DAD-ESI-MSn, and evaluated regarding bioactive properties (antioxidant and antibacterial). Flavonol and flavone glycoside derivatives and phenolic acids, specially rosmarinic acid and kaempferol-O-glucuronide were the major compounds in T. algeriensis extracts. Otherwise, E. alata presented isoflavones and flavonol derivatives as main compounds, being hydroxypuerarin isomer 1 the major molecule. Aqueous extracts had significantly higher antioxidant activity, being this activity correlated with the amount of phenolic compounds. Antimicrobial activity of the extracts was tested against multi-resistant bacteria strains from clinical isolates. The obtained MIC values indicate that the hydroethanolic extracts revealed the highest effect, especially the one of T. algeriensis against Gram-positive bacteria. Hence, these plant extracts could be used in the development of bioactive ingredients.