Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering

Detalhes bibliográficos
Autor(a) principal: Manjua, Ana C.
Data de Publicação: 2021
Outros Autores: Cabral, Joaquim M. S., Portugal, Carla A. M., Ferreira, Frederico Castelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/145958
Resumo: The authors acknowledge the financial support from Fundação para a Ciência e a Tecnologia (FCT-MEC), Portugal, through the dedicated project [PTDC/EDM-EDM/30828/2017] (BeLive) and PhD grant [SFRH/BD/114043/2015] and  through the project [EXPL/CTM-POL/1117/1135/2012] Moreover, the authors thanks POR Lisboa 2020 for the research project [PRECISE, Project N. 16394]. We acknowledge Dr. Marta Teixeira and the IPATIMUP facilities for the development of the ex vivo CAM experiments. The authors acknowledge Prof. Reyes Mallada (University of Zaragoza, Spain) for the use of the vibrating sample magnetometer (VSM) equipment and Dr. Pavel Strichovanec (University of Zaragoza, Spain) for the technical assistance provided during the experiments. We also acknowledge the Instituto de Medicina Molecular (IMM, Lisboa) for the services provided concerning the use of the Confocal Scanning Microscopy (Zeiss LSM 710). Publisher Copyright: © 2021 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis Group.
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spelling Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering211 Scaffold / Tissue engineering/Drug delivery212 Surface and interfaces30 Bio-inspired and biomedical materialsangiogenesisMagnetic fieldMSCsscaffoldsVEGFMaterials Science(all)The authors acknowledge the financial support from Fundação para a Ciência e a Tecnologia (FCT-MEC), Portugal, through the dedicated project [PTDC/EDM-EDM/30828/2017] (BeLive) and PhD grant [SFRH/BD/114043/2015] and  through the project [EXPL/CTM-POL/1117/1135/2012] Moreover, the authors thanks POR Lisboa 2020 for the research project [PRECISE, Project N. 16394]. We acknowledge Dr. Marta Teixeira and the IPATIMUP facilities for the development of the ex vivo CAM experiments. The authors acknowledge Prof. Reyes Mallada (University of Zaragoza, Spain) for the use of the vibrating sample magnetometer (VSM) equipment and Dr. Pavel Strichovanec (University of Zaragoza, Spain) for the technical assistance provided during the experiments. We also acknowledge the Instituto de Medicina Molecular (IMM, Lisboa) for the services provided concerning the use of the Confocal Scanning Microscopy (Zeiss LSM 710). Publisher Copyright: © 2021 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis Group.The growing prevalence of vascular diseases worldwide has emphasized the need for novel tissue-engineered options concerning the development of vascularized 3D constructs. This study reports, for the first time, the use of external magnetic fields to stimulate mesenchymal stromal cells (MSCs) to increase the production of vascular endothelial growth factor-A (VEGF-A). Polyvinylalcohol and gelatin-based scaffolds, containing iron oxide nanoparticles, were designed for optimal cell magnetic stimulation. While the application of static magnetic fields over 24 h did not impact on MSCs proliferation, viability and phenotypic identity, it significantly increased the production of VEGF-A and guided MSCs morphology and alignment. The ability to enhance MSCs angiogenic potential was demonstrated by the increase in the number of new vessels formed in the presence of MSCs conditioned media through in vitro and in vivo models. Ultimately, this study uncovers the potential to manipulate cellular processes through short-term magnetic stimulation.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNManjua, Ana C.Cabral, Joaquim M. S.Portugal, Carla A. M.Ferreira, Frederico Castelo2022-12-02T22:14:30Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://hdl.handle.net/10362/145958eng1468-6996PURE: 45610372https://doi.org/10.1080/14686996.2021.1927834info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:26:49Zoai:run.unl.pt:10362/145958Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:22.164383Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering
title Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering
spellingShingle Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering
Manjua, Ana C.
211 Scaffold / Tissue engineering/Drug delivery
212 Surface and interfaces
30 Bio-inspired and biomedical materials
angiogenesis
Magnetic field
MSCs
scaffolds
VEGF
Materials Science(all)
title_short Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering
title_full Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering
title_fullStr Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering
title_full_unstemmed Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering
title_sort Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering
author Manjua, Ana C.
author_facet Manjua, Ana C.
Cabral, Joaquim M. S.
Portugal, Carla A. M.
Ferreira, Frederico Castelo
author_role author
author2 Cabral, Joaquim M. S.
Portugal, Carla A. M.
Ferreira, Frederico Castelo
author2_role author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Manjua, Ana C.
Cabral, Joaquim M. S.
Portugal, Carla A. M.
Ferreira, Frederico Castelo
dc.subject.por.fl_str_mv 211 Scaffold / Tissue engineering/Drug delivery
212 Surface and interfaces
30 Bio-inspired and biomedical materials
angiogenesis
Magnetic field
MSCs
scaffolds
VEGF
Materials Science(all)
topic 211 Scaffold / Tissue engineering/Drug delivery
212 Surface and interfaces
30 Bio-inspired and biomedical materials
angiogenesis
Magnetic field
MSCs
scaffolds
VEGF
Materials Science(all)
description The authors acknowledge the financial support from Fundação para a Ciência e a Tecnologia (FCT-MEC), Portugal, through the dedicated project [PTDC/EDM-EDM/30828/2017] (BeLive) and PhD grant [SFRH/BD/114043/2015] and  through the project [EXPL/CTM-POL/1117/1135/2012] Moreover, the authors thanks POR Lisboa 2020 for the research project [PRECISE, Project N. 16394]. We acknowledge Dr. Marta Teixeira and the IPATIMUP facilities for the development of the ex vivo CAM experiments. The authors acknowledge Prof. Reyes Mallada (University of Zaragoza, Spain) for the use of the vibrating sample magnetometer (VSM) equipment and Dr. Pavel Strichovanec (University of Zaragoza, Spain) for the technical assistance provided during the experiments. We also acknowledge the Instituto de Medicina Molecular (IMM, Lisboa) for the services provided concerning the use of the Confocal Scanning Microscopy (Zeiss LSM 710). Publisher Copyright: © 2021 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis Group.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2022-12-02T22:14:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/145958
url http://hdl.handle.net/10362/145958
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1468-6996
PURE: 45610372
https://doi.org/10.1080/14686996.2021.1927834
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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