Development of a microfluidics droplet generator

• Autor(a) principal: Matos, Francisco Bernardo Gomes Filipe de
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/58116
• topic: Microfluidics; Nano droplet generator; X-Junction; T-Junction; Y-Junction; Dripping; Domínio/Área Científica::Engenharia e Tecnologia::Engenharia dos Materiais

The need for mass scale testing in areas such as microbiology and chemistry requires faster processing times, multiplexing capability, and reduced reagent requirements. To achieve this, the volumes processed must be reduced. This work intends to produce a microfluidic chip capable of producing increasingly smaller droplets that serve as testing vessels, by taking advantage of the dynamics of two immiscible fluids. The purpose of the present chip is to be used in the future in a digital Polymerised Chain Reaction (dPCR) for DNA amplification and detection. The microfluidic device was first simulated using COMSOL Multiphysics to understand the different behaviours of the droplet generator junctions. Glass sealed devices were produced using soft-lithography, composed of two different parts, a glass substrate and a top PDMS slab fabricated by photolithography of a SU-8 mould on a Si wafer that was used to mould the PDMS. Devices were tested with two immiscible fluids, which were injected at a constant flow rate into two inlets that lead to the junction were the droplets were formed. We were able to obtain droplets as small as 1 nL in devices with a channel size of 50 μm. We concluded that reducing the entry section to the main channel until the junction point, will decrease droplet size keeping the same size of the channels after the junction. Faster droplet generation rate was also obtained, using side channels width smaller (50 μm) than the main channel (100 μm).