Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/5861 |
Resumo: | A brewer’s yeast strain was grown with maltose as sole carbon source under strictly anaerobic conditions with and without ergosterol and /or unsaturated fatty acid (Tween 80) supplements. Under all these conditions the MALx1 genes for maltose transporters were strongly expressed during growth. The fatty acid unsaturation indices of growing and stationary phase yeast were increased from about 20% to 56–69% by supplementation with Tween 80. Ergosterol contents were increased up to at least 4- fold by supplementation with ergosterol and Tween 80. Maltose transport activity measured at 20°C was not increased by supplementation with Tween 80 alone, but was increased 2-fold and 3-fold, respectively, in growing and stationary phase yeast by supplementation with ergosterol together with Tween 80. The stimulation of maltose transport by ergosterol was greater when the transport was measured at temperatures (10°C and 0°C) lower than 20°C. The results show that proper function of maltose transporters requires adequate amounts of ergosterol in the yeast. This effect may partly explain the low maltose (and maltotriose) uptake rates both in the second half of brewery fermentations, when the sterol content of yeast has fallen, and when fresh wort is pitched with sterol-deficient cropped yeast. |
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Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeastCropped yeastErgosterolLipid compositionMaltose transportTemperature effectsUnsaturated fatty acidsA brewer’s yeast strain was grown with maltose as sole carbon source under strictly anaerobic conditions with and without ergosterol and /or unsaturated fatty acid (Tween 80) supplements. Under all these conditions the MALx1 genes for maltose transporters were strongly expressed during growth. The fatty acid unsaturation indices of growing and stationary phase yeast were increased from about 20% to 56–69% by supplementation with Tween 80. Ergosterol contents were increased up to at least 4- fold by supplementation with ergosterol and Tween 80. Maltose transport activity measured at 20°C was not increased by supplementation with Tween 80 alone, but was increased 2-fold and 3-fold, respectively, in growing and stationary phase yeast by supplementation with ergosterol together with Tween 80. The stimulation of maltose transport by ergosterol was greater when the transport was measured at temperatures (10°C and 0°C) lower than 20°C. The results show that proper function of maltose transporters requires adequate amounts of ergosterol in the yeast. This effect may partly explain the low maltose (and maltotriose) uptake rates both in the second half of brewery fermentations, when the sterol content of yeast has fallen, and when fresh wort is pitched with sterol-deficient cropped yeast.Finnish brewing and malting industry (PBL) ; Leonardo da Vinci programme ; Fundação para a Ciência e a Tecnologia (FCT)Institute of Brewing & DistillingUniversidade do MinhoGuimarães, Pedro M. R.Virtanen, HanneleLondesborough, John20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/5861eng"Journal of the Institute of Brewing". ISSN 0046-9750. 112:3 (2006) 203-209.0046-975010.1002/j.2050-0416.2006.tb00714.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:26:45Zoai:repositorium.sdum.uminho.pt:1822/5861Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:21:14.455657Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast |
title |
Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast |
spellingShingle |
Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast Guimarães, Pedro M. R. Cropped yeast Ergosterol Lipid composition Maltose transport Temperature effects Unsaturated fatty acids |
title_short |
Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast |
title_full |
Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast |
title_fullStr |
Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast |
title_full_unstemmed |
Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast |
title_sort |
Direct evidence that maltose transport activity Is affected by the lipid composition of brewer’s yeast |
author |
Guimarães, Pedro M. R. |
author_facet |
Guimarães, Pedro M. R. Virtanen, Hannele Londesborough, John |
author_role |
author |
author2 |
Virtanen, Hannele Londesborough, John |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Guimarães, Pedro M. R. Virtanen, Hannele Londesborough, John |
dc.subject.por.fl_str_mv |
Cropped yeast Ergosterol Lipid composition Maltose transport Temperature effects Unsaturated fatty acids |
topic |
Cropped yeast Ergosterol Lipid composition Maltose transport Temperature effects Unsaturated fatty acids |
description |
A brewer’s yeast strain was grown with maltose as sole carbon source under strictly anaerobic conditions with and without ergosterol and /or unsaturated fatty acid (Tween 80) supplements. Under all these conditions the MALx1 genes for maltose transporters were strongly expressed during growth. The fatty acid unsaturation indices of growing and stationary phase yeast were increased from about 20% to 56–69% by supplementation with Tween 80. Ergosterol contents were increased up to at least 4- fold by supplementation with ergosterol and Tween 80. Maltose transport activity measured at 20°C was not increased by supplementation with Tween 80 alone, but was increased 2-fold and 3-fold, respectively, in growing and stationary phase yeast by supplementation with ergosterol together with Tween 80. The stimulation of maltose transport by ergosterol was greater when the transport was measured at temperatures (10°C and 0°C) lower than 20°C. The results show that proper function of maltose transporters requires adequate amounts of ergosterol in the yeast. This effect may partly explain the low maltose (and maltotriose) uptake rates both in the second half of brewery fermentations, when the sterol content of yeast has fallen, and when fresh wort is pitched with sterol-deficient cropped yeast. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2006-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/5861 |
url |
http://hdl.handle.net/1822/5861 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of the Institute of Brewing". ISSN 0046-9750. 112:3 (2006) 203-209. 0046-9750 10.1002/j.2050-0416.2006.tb00714.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Brewing & Distilling |
publisher.none.fl_str_mv |
Institute of Brewing & Distilling |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132678629359616 |