Acacia longifolia: A Host of Many Guests Even after Fire
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/45523 |
Resumo: | Acacia longifolia is a worldwide invader that cause damage in ecosystems, expanding largely after wildfires, which promote germination of a massive seed bank. As a legume, symbiosis is determinant for adaptation. Our study aims to isolate a wider consortium of bacteria harboured in nodules, including both nitrogen and non-nitrogen fixers. Furthermore, we aim to evaluate the e ects of fire in nodulation and bacterial diversity on young acacias growing in unburnt and burnt zones, one year after the fire. For this, we used molecular approaches, M13 fingerprinting and 16S rRNA partial sequencing, to identify species/genera involved and 15N isotopic composition in leaves and plant nodules. Nitrogen isotopic analyses in leaves suggest that in unburnt zones, nitrogen fixation contributes more to plant nitrogen content. Overall, A. longifolia seems to be promiscuous and despite Bradyrhizobium spp. dominance, Paraburkholderia spp. followed by Pseudomonas spp. was also found. Several species not previously reported as nitrogen-fixers were identified, proposing other functions besides ammonia acquisition. Our study shows that bacterial communities are di erent in nodules after fire. Fire seems to potentiate nodulation and drives symbiosis towards nitrogen-fixers. Taken together, a multifunctional community inside nodules is pointed out which potentiate A. longifolia invasiveness and adaptation. |
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Acacia longifolia: A Host of Many Guests Even after Fireclimate changeinvasive speciesnodulationsymbiosisBradyrhizobium spp.Acacia longifolia is a worldwide invader that cause damage in ecosystems, expanding largely after wildfires, which promote germination of a massive seed bank. As a legume, symbiosis is determinant for adaptation. Our study aims to isolate a wider consortium of bacteria harboured in nodules, including both nitrogen and non-nitrogen fixers. Furthermore, we aim to evaluate the e ects of fire in nodulation and bacterial diversity on young acacias growing in unburnt and burnt zones, one year after the fire. For this, we used molecular approaches, M13 fingerprinting and 16S rRNA partial sequencing, to identify species/genera involved and 15N isotopic composition in leaves and plant nodules. Nitrogen isotopic analyses in leaves suggest that in unburnt zones, nitrogen fixation contributes more to plant nitrogen content. Overall, A. longifolia seems to be promiscuous and despite Bradyrhizobium spp. dominance, Paraburkholderia spp. followed by Pseudomonas spp. was also found. Several species not previously reported as nitrogen-fixers were identified, proposing other functions besides ammonia acquisition. Our study shows that bacterial communities are di erent in nodules after fire. Fire seems to potentiate nodulation and drives symbiosis towards nitrogen-fixers. Taken together, a multifunctional community inside nodules is pointed out which potentiate A. longifolia invasiveness and adaptation.MDPI; ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLANDRepositório da Universidade de LisboaJesus, JoanaTenreiro, R.Máguas, C.Trindade, Helena2020-12-21T21:29:27Z2020-06-092020-06-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/45523engJesus, J.G.; Tenreiro, R.; Máguas, C.; Trindade, H. Acacia longifolia: A Host of Many Guests Even after Fire. Diversity 2020, 12, 250.10.3390/d12060250info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:47:24Zoai:repositorio.ul.pt:10451/45523Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:57:55.968906Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Acacia longifolia: A Host of Many Guests Even after Fire |
title |
Acacia longifolia: A Host of Many Guests Even after Fire |
spellingShingle |
Acacia longifolia: A Host of Many Guests Even after Fire Jesus, Joana climate change invasive species nodulation symbiosis Bradyrhizobium spp. |
title_short |
Acacia longifolia: A Host of Many Guests Even after Fire |
title_full |
Acacia longifolia: A Host of Many Guests Even after Fire |
title_fullStr |
Acacia longifolia: A Host of Many Guests Even after Fire |
title_full_unstemmed |
Acacia longifolia: A Host of Many Guests Even after Fire |
title_sort |
Acacia longifolia: A Host of Many Guests Even after Fire |
author |
Jesus, Joana |
author_facet |
Jesus, Joana Tenreiro, R. Máguas, C. Trindade, Helena |
author_role |
author |
author2 |
Tenreiro, R. Máguas, C. Trindade, Helena |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Jesus, Joana Tenreiro, R. Máguas, C. Trindade, Helena |
dc.subject.por.fl_str_mv |
climate change invasive species nodulation symbiosis Bradyrhizobium spp. |
topic |
climate change invasive species nodulation symbiosis Bradyrhizobium spp. |
description |
Acacia longifolia is a worldwide invader that cause damage in ecosystems, expanding largely after wildfires, which promote germination of a massive seed bank. As a legume, symbiosis is determinant for adaptation. Our study aims to isolate a wider consortium of bacteria harboured in nodules, including both nitrogen and non-nitrogen fixers. Furthermore, we aim to evaluate the e ects of fire in nodulation and bacterial diversity on young acacias growing in unburnt and burnt zones, one year after the fire. For this, we used molecular approaches, M13 fingerprinting and 16S rRNA partial sequencing, to identify species/genera involved and 15N isotopic composition in leaves and plant nodules. Nitrogen isotopic analyses in leaves suggest that in unburnt zones, nitrogen fixation contributes more to plant nitrogen content. Overall, A. longifolia seems to be promiscuous and despite Bradyrhizobium spp. dominance, Paraburkholderia spp. followed by Pseudomonas spp. was also found. Several species not previously reported as nitrogen-fixers were identified, proposing other functions besides ammonia acquisition. Our study shows that bacterial communities are di erent in nodules after fire. Fire seems to potentiate nodulation and drives symbiosis towards nitrogen-fixers. Taken together, a multifunctional community inside nodules is pointed out which potentiate A. longifolia invasiveness and adaptation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-21T21:29:27Z 2020-06-09 2020-06-09T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/45523 |
url |
http://hdl.handle.net/10451/45523 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Jesus, J.G.; Tenreiro, R.; Máguas, C.; Trindade, H. Acacia longifolia: A Host of Many Guests Even after Fire. Diversity 2020, 12, 250. 10.3390/d12060250 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI; ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND |
publisher.none.fl_str_mv |
MDPI; ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134524545695744 |