Differential response to isoproturon by two strains of Saccharomyces cerevisiae.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/4034 |
Resumo: | S. cerevisiae UE-ME3 grown in MB medium in the absence and presence of IPU show a similar growth profile and a stabilization of the biomass, non-protein thiols content, cell viability (cfu) and cell redox status estimated by GSH/GSSG ratio. This response seems depend on GR and G6PD activities increase, which probably activates the glutathione cycle, signs of adaptive response to phenylurea. However, S. cerevisiae IGC-4072 strain showed signs of cell death in the presence of IPU, exhibiting a more slowly growth profile, a decrease of dry weight and cell viability, as well as a significant increase of free radicals scavenger (DPPH) in presence of phenylurea, without significant changes of the GSH/GSSG ratio. As the value of the GSH/GSSG ratio is significant lower in IGC-4072 cells and GR activity undergoes a marked decrease in the presence of the IPU it is suspected that the worst IPU adaptive capacity displayed by this strain depends on a poor antioxidant power mediated by the glutathione. |
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Differential response to isoproturon by two strains of Saccharomyces cerevisiae.UE-ME3IGC-4072antioxidant capacityS. cerevisiae UE-ME3 grown in MB medium in the absence and presence of IPU show a similar growth profile and a stabilization of the biomass, non-protein thiols content, cell viability (cfu) and cell redox status estimated by GSH/GSSG ratio. This response seems depend on GR and G6PD activities increase, which probably activates the glutathione cycle, signs of adaptive response to phenylurea. However, S. cerevisiae IGC-4072 strain showed signs of cell death in the presence of IPU, exhibiting a more slowly growth profile, a decrease of dry weight and cell viability, as well as a significant increase of free radicals scavenger (DPPH) in presence of phenylurea, without significant changes of the GSH/GSSG ratio. As the value of the GSH/GSSG ratio is significant lower in IGC-4072 cells and GR activity undergoes a marked decrease in the presence of the IPU it is suspected that the worst IPU adaptive capacity displayed by this strain depends on a poor antioxidant power mediated by the glutathione.Formatex2012-01-23T15:22:39Z2012-01-232011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/4034http://hdl.handle.net/10174/4034engCandeias M, Alves-Pereira I, Ferreira R (2011) Differential response to isoproturon by two strains of Saccharomyces cerevisiae. IV International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2011), Torremolinos-Malaga (Spain)http://www.formatex.info/biomicroworld2011/acceptedabstracts.phpICAAMndiap@uevora.ptraf@uevora.pt365Candeias, MAlves-Pereira, IFerreira, Rinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:41:32Zoai:dspace.uevora.pt:10174/4034Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:59:19.404259Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differential response to isoproturon by two strains of Saccharomyces cerevisiae. |
title |
Differential response to isoproturon by two strains of Saccharomyces cerevisiae. |
spellingShingle |
Differential response to isoproturon by two strains of Saccharomyces cerevisiae. Candeias, M UE-ME3 IGC-4072 antioxidant capacity |
title_short |
Differential response to isoproturon by two strains of Saccharomyces cerevisiae. |
title_full |
Differential response to isoproturon by two strains of Saccharomyces cerevisiae. |
title_fullStr |
Differential response to isoproturon by two strains of Saccharomyces cerevisiae. |
title_full_unstemmed |
Differential response to isoproturon by two strains of Saccharomyces cerevisiae. |
title_sort |
Differential response to isoproturon by two strains of Saccharomyces cerevisiae. |
author |
Candeias, M |
author_facet |
Candeias, M Alves-Pereira, I Ferreira, R |
author_role |
author |
author2 |
Alves-Pereira, I Ferreira, R |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Candeias, M Alves-Pereira, I Ferreira, R |
dc.subject.por.fl_str_mv |
UE-ME3 IGC-4072 antioxidant capacity |
topic |
UE-ME3 IGC-4072 antioxidant capacity |
description |
S. cerevisiae UE-ME3 grown in MB medium in the absence and presence of IPU show a similar growth profile and a stabilization of the biomass, non-protein thiols content, cell viability (cfu) and cell redox status estimated by GSH/GSSG ratio. This response seems depend on GR and G6PD activities increase, which probably activates the glutathione cycle, signs of adaptive response to phenylurea. However, S. cerevisiae IGC-4072 strain showed signs of cell death in the presence of IPU, exhibiting a more slowly growth profile, a decrease of dry weight and cell viability, as well as a significant increase of free radicals scavenger (DPPH) in presence of phenylurea, without significant changes of the GSH/GSSG ratio. As the value of the GSH/GSSG ratio is significant lower in IGC-4072 cells and GR activity undergoes a marked decrease in the presence of the IPU it is suspected that the worst IPU adaptive capacity displayed by this strain depends on a poor antioxidant power mediated by the glutathione. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01T00:00:00Z 2012-01-23T15:22:39Z 2012-01-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/4034 http://hdl.handle.net/10174/4034 |
url |
http://hdl.handle.net/10174/4034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Candeias M, Alves-Pereira I, Ferreira R (2011) Differential response to isoproturon by two strains of Saccharomyces cerevisiae. IV International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2011), Torremolinos-Malaga (Spain) http://www.formatex.info/biomicroworld2011/acceptedabstracts.php ICAAM nd iap@uevora.pt raf@uevora.pt 365 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Formatex |
publisher.none.fl_str_mv |
Formatex |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136476062023680 |